Serum-free culture of Pasteurella haemolytica optimized for leukotoxin production

American Journal of Veterinary Research

Volumn 59, Issue 7, 1998, Pages 851-855

  Sun, Yude ^a   Clinkenbeard, Kenneth D ^a  

^a OKLAHOMA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL TOXIN; EXOTOXIN; FERRIC CHLORIDE; FERRIC ION; LEUKOTOXIN; MAGNESIUM SULFATE;

ANIMAL; ARTICLE; BIOSYNTHESIS; CATTLE; CULTURE MEDIUM; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; LUNG; MANNHEIMIA HAEMOLYTICA; METABOLISM; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY;

ANIMALS; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CATTLE; CULTURE MEDIA, SERUM-FREE; EXOTOXINS; FERRIC COMPOUNDS; LUNG; MAGNESIUM SULFATE; MANNHEIMIA HAEMOLYTICA;

EID: 0032111936     PISSN: 00029645     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Carter GR, Chengappa MM, Roberts AW. Essentials of veterinary microbiology. Baltimore: The Williams & Wilkins Co, 1995; 171-179.
2. Confer AW, Panciera RJ, Mosier DA. Bovine pneumonic pasteurellosis: immunity to Pasteurella haemolytica. J Am Vet Med Assoc 1988;193:1308-1316.
3. Shewen PE, Wilkie BN. Cytotoxin of Pasteurella haemolytica acting on bovine leukocytes. Infect Immun 1982;35:91-94.
4. Shewen PE, Wilkie BN. Evidence for the Pasteurella haemolytica cytotoxin as a product of actively growing bacteria. Am J Vet Res 1985;46:1212-1214.
5. Highlander SK. Growth of Pasteurella haemolytica and production of its leukotoxin in semi-defined media. Am J Vet Res 1997; 58:749-754.
6. Gatewood DM, Fenwick BW, Chengappa MM. Growth-condition dependent expression of Pasteurella haemolytica A1 outer membrane proteins, capsule, and leukotoxin. Vet Microbiol 1994;41: 221-233.
7. Benson ML, Thomson RG, Valli VEO. The bovine alveolar macrophage II. In vitro studies with Pasteurella haemolytica. Can J Comp Med 1978;42:368-369.
8. Confer AW, Durham JA. Sequential development of antigens and toxins of Pasteurella haemolytica serotype 1 grown in cell culture medium. Am J Vet Res 1992;53:646-652.
9. Gentry MJ, Confer AW, Weinberg ED, et al. Cytotoxin (leukotoxin) production by Pasteurella haemolytica: requirement for an iron-containing compound. Am J Vet Res 1986;47:1919-1923.
10. Waurzyniak BJ, Clinkenbeard KD, Confer AW, et al. Enhancement of Pasteurella haemolytica leukotoxic activity by bovine serum albumin. Am J Vet Res 1994;55:1267-1274.
11. Gentry MJ, Srikumaran S. Neutralizing monoclonal antibodies to Pasteurella haemolytica leukotoxin affinity-purify the toxin from crude culture supernatants. Microb Pathog 1994;10:411-417.
12. Rose AH. Chemical microbiology, an introduction to microbial physiology. New York: Plenum Press, 1976;5-20.
13. Hughes MN, Poole RK. Metals and micro-organisms. New York: Chapman & Hall Ltd, 1989;3-38.
14. Utkilen HC. Magnesium-limited growth of cyanobacterium Anacytis nidulans. J Gen Microbiol 1982;128:1849-1862.
15. Vrdoljak M, Maria V, Johnnides V. Copper requirement and its influence on the growth of the obligate methylotrophic bacterial strain 4025. Biotech Lett 1984;6:501-506.
16. Wilson DO, Reisenauer HM. Effect of manganese and zinc ions on the growth of Rhizobium. J Bacteriol 1970;102:729-732.
17. Stetter KO, Kandler O. Manganese requirement of the transcription processes in Lactobacillus curvatus. FEBS Lett 1973;36:5-8.
18. Clinkenbeard KD, Mosier DA, Confer AW. Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasteuretta haemolytica leukotoxin. Infect Immun 1989;57:420-425.
19. Murphy GK, Whitworth LC, Clinkenbeard KD, et al. Hemolytic activity of Pasteurella haemolytica leukotoxin. Infect Immun 1995;63:3209-3212.
20. Clinkenbeard KD, Clarke CR, Hague C, et al. Pasteurella haemolytica leukotoxin induces leukotriene B4 synthesis by isolated bovine neutrophils. J Leukoc Biol 1994;56:644-649.
21. Wessman GE. Cultivation of Pasteurella haemolytica in a casein hydrolysate medium. Appl Microbiol 1965;13:426-431.