Purification of a big defensin from Ruditapes philippinesis and its antibacterial activity

Acta Biochimica et Biophysica Sinica

Volumn 35, Issue 12, 2003, Pages 1145-1148

  Wei, Yu Xi ^a   Guo, Dao Sen ^a   Li, Rong Gui ^a   Chen, Hao Wen ^b   Chen, Pei Xun ^a  

^a QINGDAO UNIVERSITY   (China)

^b FIRST INSTITUTE OF OCEANOGRAPHY   (China)

Author keywords

Amino acid sequence; Antibacterial activity; Purification; Ruditapes philippinesis defensin

Indexed keywords

AMMONIUM SULFATE; ANTIINFECTIVE AGENT; DEFENSIN;

AMINO ACID SEQUENCE; ANTIBACTERIAL ACTIVITY; ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; DRUG ISOLATION; DRUG PURIFICATION; ESCHERICHIA COLI; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; ION EXCHANGE CHROMATOGRAPHY; MICROCOCCUS; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; SEQUENCE HOMOLOGY; STAPHYLOCOCCUS AUREUS; VIBRIO ANGUILLARUM; VIBRIO PARAHEMOLYTICUS;

AMINO ACID SEQUENCE; ANIMALS; ANTI-BACTERIAL AGENTS; BIVALVIA; BLOOD PROTEINS; CHROMATOGRAPHY; DEFENSINS; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; MICROBIAL SENSITIVITY TESTS; SEQUENCE ANALYSIS, PROTEIN;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LISTONELLA ANGUILLARUM; MICROCOCCUS; NEGIBACTERIA; POSIBACTERIA; RUDITAPES; STAPHYLOCOCCUS; STAPHYLOCOCCUS AUREUS; VIBRIO; VIBRIO PARAHAEMOLYTICUS;

EID: 1642320397     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Destoumieux D, Bulet P, Loew D, Loew D, van Dorsselaer A, Rodriguez J, Bachère E. Penaeidins, a new family of antimicrobial peptides isolated from the shrimp Penaeus vannamei (Decapoda). J Biol Chem, 1997, 272 (45): 28398-28406
2. Roch P. Defense mechanisms and disease prevention in farmed marine invertbrates. Aquaculture, 1999, 172: 125-145
3. Hubert F, Noël T, Roch P. A member of the arthropod defensin family from edible Mediterranean mussels (Mytilus galloprovincialis). Eur J Biochem, 1996, 240(1): 302-306
4. Schnapp D, Kemp GD, Smith VJ. Purification and characterization of a proline-rich antibacterial peptide, with sequence similarity to bactenecin-7, from the haemocytes of the shore crab, Carcinus maenas. Eur J Biochem, 1996, 240(3): 532-539
5. Mitta G, Vandenbulcke F, Hubert F, Salzet M, Roch P. Involvement of mytilins in mussel antimicrobial defense. J Biol Chem, 2000, 275 (17): 12954-12962
6. Charlet M, Chernysh S, Philippe H, Hetru C, Hoffmann JA, Bulet P. Isolation of several cysteine-rich antimicrobial peptides from the blood of a mollusk Mytilis edulis. J Biol Chem, 1996, 271 (36): 21808-21813
7. Mitta G, Vandenbulcke F, Hubert F, Roch P. Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. J Cell Sci, 1999, 112: 4233-4242
8. Yang YS, Mitta G, Chavanieu A, Calas B, Sanchez JF, Roch P, Aumelas A. Solution structure and activity of the synthetic four-disulfide bond Mediterranean mussel defensin (MGD-1). Biochemistry, 2000, 39(47): 14436-14447
9. Mitta G, Hubert F, Noül T, Roch P. Myticin, a novel cysteine-rich antimicrobial peptide isolated from haemocytes and plasma of the mussel Mytilus galloprovincialis. Eur J Biochem, 1999, 265(1): 71 -78
10. Shi JH, Zhao YT, Zhang YQ, Yan Z, Han W, Wang JL. Study on analyzing methods of low molecular peptides with SDS-poryacrylamide gel electrophoresis. Prog Biotech, 2001, 21(1): 38-41
11. Wei YX, Guo DS, Li L, Chen HW. Factors affecting antimicrobial activity of antimicrobial substances in hemolymph from Ruditapes philippinarum. Chin J Appl Environ Biol, 2003, 9(3): 271-272
12. Bulet P, Cociancich S, Dimarcq JL, Lambert J, Reichhart JM, Hoffmann D, Hetru C, Hoffmann JA. Isolation from a coleopteran insect of a novel inducible antibacterial peptide and of new members of the insect defensin family. J Biol Chem, 1991, 266(36): 24520-24525