Differentiated human podocytes endogenously express an inhibitory isoform of vascular endothelial growth factor (VEGF165b) mRNA and protein

American Journal of Physiology - Renal Physiology

Volumn 286, Issue 4 55-4, 2004, Pages

  Cui, Tai Gen ^a,c   Foster, Rebecca R ^a   Saleem, Moin ^a   Mathieson, Peter W ^a   Gillatt, David A ^b   Bates, David O ^a   Harper, Steven J ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b SOUTHMEAD HOSPITAL   (United Kingdom)

^c PEKING UNIVERSITY FIRST HOSPITAL   (China)

Author keywords

Angiogenesis; Small interference RNA; Splicing

Indexed keywords

MESSENGER RNA; PROTEIN; VASCULOTROPIN;

ALTERNATIVE RNA SPLICING; ANGIOGENESIS; ARTICLE; CONTROLLED STUDY; GLOMERULUS; HUMAN; HUMAN CELL; PHENOTYPE; PODOCYTE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; STATISTICAL SIGNIFICANCE;

EID: 1642280409     PISSN: 1931857X     EISSN: 15221466     Source Type: Journal    
DOI: 10.1152/ajprenal.00337.2003     Document Type: Article

References (27)

1. Bailey E, Bottomley MJ, Westwell S, Pringle JH, Furness PN, Feehally J, Brenchley PE, and Harper SJ. Vascular endothelial growth factor mRNA expression in minimal change, membranous, and diabetic nephropathy demonstrated by non-isotopic in situ hybridisation. J Clin Pathol 52: 735-738, 1999.
2. 165b, an inhibitory splice variant of vascular endothelial growth factor, is down-regulated in renal cell carcinoma. Cancer Res 62: 4123-4131, 2002.
3. 2+-dependent pathway. Am J Physiol Heart Circ Physiol 273: H687-H694, 1997.
4. Bates DO, Heald RI, Curry FE, and Williams B. Vascular endothelial growth factor increases Rana vascular permeability and compliance by different signalling pathways. J Physiol 533: 263-272, 2001.
5. Brown LF, Berse B, Tognazzi K, Manseau EJ, Van de Water L, Senger DR, Dvorak HF, and Rosen S. Vascular permeability factor mRNA and protein expression in human kidney. Kidney Int 42: 1457-1461, 1992.
6. Chomczynscki P and Sacchi N. Single-step method of RNA isolation by acid quanidinium thiocyanate phenol chloroform extraction. Anal Biochem 162: 156-159, 1987.
7. De Vriese AS, Tilton RG, Elger M, Stephan CC, Kriz W, and Lameire NH. Antibodies against vascular endothelial growth factor improve early renal dysfunction in experimental diabetes. J Am Soc Nephrol 12: 993-1000, 2001.
8. Dvorak HF. Vascular permeability factor/vascular endothelial growth factor: a critical cytokine in tumor angiogenesis and a potential target for diagnosis and therapy. J Clin Oncol 20: 4368-4380, 2002.
9. Eremina V, Sood M, Haigh J, Nagy A, Lajoie G, Ferrara N, Gerber HP, Kikkawa Y, Miner JH, and Quaggin SE. Glomerular-specific alterations of VEGF-A expression lead to distinct congenital and acquired renal diseases. J Clin Invest 111: 707-716, 2003.
10. Feng D, Nagy JA, Brekken RA, Pettersson A, Manseau EJ, Pyne K, Mulligan R, Thorpe PE, Dvorak HF, and Dvorak AM. Ultrastructural localization of the vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) receptor-2 (FLK-1, KDR) in normal mouse kidney and in the hyperpermeable vessels induced by VPF/VEGF-expressing tumors and adenoviral vectors. J Histochem Cytochem 48: 545-556, 2000.
11. Ferrara N. Role of vascular endothelial growth factor in physiologic and pathologic angiogenesis: therapeutic implications. Semin Oncol 29: 10-14, 2002.
12. Ferrara N and Davis-Smyth T. The biology of vascular endothelial growth factor. Endocr Rev 18: 4-25, 1997.
13. Foster RR, Hole R, Anderson K, Satchell SC, Coward RJ, Mathieson PW, Gillatt DA, Saleem MA, Bates DO, and Harper SJ. Functional evidence that vascular endothelial growth factor may act as an autocrine factor on human podocytes. Am J Physiol Renal Physiol 284: F1263-F1273, 2003.
14. Fuh G, Garcia KC, and de Vos AM. The interaction of neuropilin-1 with vascular endothelial growth factor and its receptor flt-1. J Biol Chem 275: 26690-26695, 2000.
15. Harper SJ, Bailey E, McKeen CM, Stewart AS, Pringle JH, Feehally J, and Brown T. A comparative study of digoxigenin, 2,4-dinitrophenyl, and alkaline phosphatase as deoxyoligonucleotide labels in non-radioisotopic in situ hybridisation. J Clin Pathol 50: 686-690, 1997.
16. Harper SJ, Xing CY, Whittle C, Parry R, Gillatt D, Peat D, and Mathieson PW. Expression of neuropilin-1 by human glomerular epithelial cells in vitro and in vivo. Clin Sci (Lond) 101: 439-446, 2001.
17. Nicol D, Hii SI, Walsh M, Teh B, Thompson L, Kennett C, and Gotley D. Vascular endothelial growth factor expression is increased in renal cell carcinoma. J Urol 157: 1482-1486, 1997.
18. 165 mediates glomerular endothelial repair. J Clin Invest 104: 913-923, 1999.
19. Paddison PJ and Hannon GJ. RNA interference: the new somatic cell genetics? Cancer Cell 2: 17-23, 2002.
20. Parry RG, Gillespie KM, and Mathieson PW. Effects of type 2 cytokines on glomerular epithelial cells. Exp Nephrol 9: 275-283, 2001.
21. Saleem MA, O'Hare MJ, Reiser J, Coward RJ, Inward CD, Farren T, Xing CY, Ni L, Mathieson PW, and Mundel P. A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol 13: 630-638, 2002.
22. Satchell SC, Harper SJ, Tooke JE, Kerjaschki D, Saleem MA, and Mathieson PW. Human podocytes express angiopoietin 1, a potential regulator of glomerular vascular endothelial growth factor. J Am Soc Nephrol 13: 544-550, 2002.
23. Siddiqui FA, Desai H, Siddiqui TF, and Francis JL. Hemoglobin induces the expression and secretion of vascular endothelial growth factor from human malignant cells. Hematol J 3: 264-270, 2002.
24. Sugimoto H, Hamano Y, Charytan D, Cosgrove D, Kieran M, Sudhakar A, and Kalluri R. Neutralization of circulating vascular endothelial growth factor (VEGF) by anti-VEGF antibodies and soluble VEGF receptor 1 (sFlt-1) induces proteinuria. J Biol Chem 278: 12605-12608, 2003.
25. Sugiono M, Perrin RM, Harper SJ, Oxley JD, Gillatt DA, and Bates DO. Vascular endothelial growth factor (VEGF) 165b, a novel and inhibitory VEGF isoform is down-regulated in prostate carcinoma (Abstract). Kuala Lumpur, Malaysia: Asian Congress of Urological Cancer, Kuala Lumpur, 2002.
26. Tricarico C, Salvadori B, Villari D, Nicita G, Della Melina A, Pinzani P, Ziche M, and Pazzagli M. Quantitative RT-PCR assay for VEGF mRNA in human tumors of the kidney. Int J Biol Markers 14: 247-250, 1999.
27. Whittle C, Gillespie K, Harrison R, Mathieson PW, and Harper SJ. Heterogeneous vascular endothelial growth factor (VEGF) isoform mRNA and receptor mRNA expression in human glomeruli, and the identification of VEGF148 mRNA, a novel truncated splice variant. Clin Sci (Lond) 97: 303-312, 1999.