The Biliverdin Chromophore Binds Covalently to a Conserved Cysteine Residue in the N-Terminus of Agrobacterium Phytochrome Agp1

Biochemistry

Volumn 43, Issue 12, 2004, Pages 3659-3669

  Lamparter, Tilman ^a   Carrascal, Montserrat ^b   Michael, Norbert ^a   Martinez, Enriqueta ^a   Rottwinkel, Gregor ^a   Abian, Joaquin ^b  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b INSTITUT D INVESTIGACIONS BIOMÈDIQUES AUGUST PI I SUNYER IDIBAPS   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; MASS SPECTROMETRY; MUTAGENESIS; PLANTS (BOTANY); PURIFICATION;

AUTOCATALYTIC LYASE REACTION; COVALENT ATTACHMENT;

PROTEINS;

BILIVERDIN; CYSTEINE; LYASE; PHYTOCHROME; PROTEIN AGP1; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; AMINO ACID ANALYSIS; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CATALYSIS; CHROMATOPHORE; COVALENT BOND; DEINOCOCCACEAE; EXPRESSION VECTOR; MASS SPECTROMETRY; NONHUMAN; PEPTIDE ANALYSIS; PHOTORECEPTOR; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN ENGINEERING; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; REACTION ANALYSIS; RHIZOBIUM; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID TRANSPORT SYSTEMS, NEUTRAL; BACTERIAL PROTEINS; BILIVERDINE; BINDING SITES; CONSERVED SEQUENCE; CYSTEINE; HISTIDINE; HYDROLYSIS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PEPTIDE FRAGMENTS; PHYTOCHROME; PROTEIN BINDING; RHIZOBIUM; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN;

AGROBACTERIUM; BACTERIA (MICROORGANISMS); CYANOBACTERIA; DEINOCOCCACEAE; DEINOCOCCUS; NEGIBACTERIA; VECTORS;

EID: 1642276701     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035693l     Document Type: Article

References (32)

1. Bhoo, S. H., Davis, S. J., Walker, J., Karniol, B., and Vierstra, R. D. (2001) Bacteriophytochromes are photochromic histidine kinases using a biliverdin chromophore, Nature 414, 776-779.
2. Lamparter, T. and Hughes, J. (in press) Phytochromes and phytochrome-like proteins in cyanobacteria, in Photoreceptors and Light Signalling (Batschauer, A., Ed.) RSC, Cambridge, U.K.
3. Starostzik, C., and Marwan, W. (1995) A photoreceptor with characteristics of phytochrome triggers sporulation in the true slime mould Physarum polycephalum, FEBS Lett. 370, 146-148.
4. Rüdiger, W., and Thümmler, F. (1994) The phytochrome chromophore, in Photomorphogenesis in Plants (Kendrick, R. E., and Kronenberg, G. H. M., Eds.) 2nd ed., pp 51-69, Kluwer Academic Publishers, Dordrecht, The Netherlands.
5. Kidd, D. G., and Lagarias, J. C. (1990) Phytochrome from the green alga Mesotaenium caldariorum. Purification and preliminary characterization, J. Biol. Chem. 12, 7029-7035.
6. Hübschmann, T., Börner, T., Hartmann, E., and Lamparter, T. (2001) Characterisation of the Cph1 holo-phytochrome from Synechocystis sp. PCC 6803, Eur. J. Biochem. 268, 2055-2063.
7. Wahleithner, J. A., Li, L. M., and Lagarias, J. C. (1991) Expression and assembly of spectrally active recombinant holophytochrome, Proc. Natl. Acad. Sci. U.S.A. 88, 10387-10391.
8. Lamparter, T., Mittmann, F., Gärtner, W., Börner, T., Hartmann, E., and Hughes, J. (1997) Characterization of recombinant phytochrome from the cyanobacterium Synechocystis, Proc. Natl. Acad. Sci. U.S.A. 94, 11792-11797.
9. Jorissen, H. J., Quest, B., Remberg, A., Coursin, T., Braslavsky, S. E., Schaffner, K., Tandeau de Marsac, N., and Gärtner, W. (2002) Two independent, light-sensing two-component systems in a filamentous cyanobacterium, Eur. J. Biochem. 269, 2662-2671.
10. Davis, S. J., Vener, A. V., and Vierstra, R. D. (1999) Bacteriophytochromes: Phytochrome-like photoreceptors from nonphotosynthetic eubacteria, Science 286, 2517-2520.
11. Lamparter, T., Michael, N., Mittmann, F., and Esteban, B. (2002) Phytochrome from Agrobacterium tumefaciens has unusual spectral properties and reveals an N-terminal chromophore attachment site, Proc. Natl. Acad. Sci. U.S.A. 99, 11628-11633.
12. Giraud, E., Fardoux, J., Fourrier, N., Hannibal, L., Genty, B., Bouyer, P., Dreyfus, B., and Vermeglio, A. (2002) Bacteriophytochrome controls photosystem synthesis in anoxygenic bacteria, Nature 417, 202-205.
13. Lamparter, T., Michael, N., Caspani, O., Miyata, T., Shirai, K., and Inomata, K. (2003) Biliverdin binds covalently to Agrobacterium phytochrome Agp1 via its ring A vinyl side chain, J. Biol. Chem. 278, 33786-33792.
14. Aravind, L., and Ponting, C. P. (1997) The GAF domain: an evolutionary link between diverse phototransducing proteins, Trends. Biochem. Sci. 22, 458-459.
15. Hughes, J., Lamparter, T., Mittmann, F., Hartmann, E., Gärtner, W., Wilde, A., and Börner, T. (1997) A prokaryotic phytochrome, Nature 386, 663.
16. Lamparter, T., Esteban, B., and Hughes, J. (2001) Phytochrome Cph1 from the cyanobacterium Synechocystis PCC6803: purification, assembly, and quaternary structure, Eur. J. Biochem. 268, 4720-4730.
17. Berkelman, T. R., and Lagarias, J. C. (1986) Visualization of bilin-linked peptides and proteins in polyacrylamide gels, Anal. Biochem. 156, 194-201.
18. Griffiths, W. J. (2000) Nanospray mass spectrometry in protein and peptide chemistry, EXS 88, 69-79.
19. Roepstorff, P. (2000) MALDI-TOF mass spectrometry in protein chemistry, EXS 88, 81-97.
20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F., and Higgins, D. G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucleic Acids Res. 24, 4876-4882.
21. Wilde, A., Churin, Y., Schubert, H., and Börner, T. (1997) Disruption of a Synechocystis sp. PCC 6803 gene with partial similarity to phytochrome genes alters growth under changing light qualities, FEBS Lett. 406, 89-92.
22. Kehoe, D. M., and Grossman, R. (1996) Similarity of a chromatic adaptation sensor to phytochrome and ethylene receptors, Science 273, 1409-1412.
23. Wu, S. H., and Lagarias, J. C. (2000) Defining the bilin lyase domain: lessons from the extended phytochrome superfamily, Biochemistry 39, 13487-13495.
24. Schmitz, O., Katayama, M., Williams, S. B., Kondo, T., and Golden, S. S. (2000) CikA, a bacteriophytochrome that resets the cyanobacterial circadian clock, Science 289, 765-768.
25. Jorissen, H. J., Quest, B., Lindner, I., Tandeau de Marsac, N., and Gärtner, W. (2002) Phytochromes with noncovalently bound chromophores: the ability of apophytochromes to direct tetrapyrrole photoisomerization, Photochem. Photobiol. 75, 554-559.
26. Allen, J. W., Tomlinson, E. J., Hong, L., and Ferguson, S. J. (2002) The Escherichia coli cytochrome c maturation (Ccm) system does not detectably attach heme to single cysteine variants of an apocytochrome c, J. Biol. Chem. 277, 33559-33563.
27. Jiang, Z. Y., Swem, L. R., Rushing, B. G., Devanathan, S., Tollin, G., and Bauer, C. E. (1999) Bacterial photoreceptor with similarity to photoactive yellow protein and plant phytochromes, Science 285, 406-409.
28. Bhoo, S. H., Hirano, T., Jeong, H. Y., Lee, J. G., Furuya, M., and Song, P.-S. (1997) Phytochrome photochromism probed by site-directed mutations and chromophore esterification, J. Am. Chem. Soc. 119, 11717-11718.
29. Remberg, A., Schmidt, P., Braslavsky, S. E., Gärtner, W., and Schaffner, K. (1999) Differential effects of mutations in the chromophore pocket of recombinant phytochrome on chromoprotein assembly and Pr-to-Pfr photoconversion, Eur. J. Biochem. 266, 201-208.
30. Deforce, L., Furuya, M., and Song, P. S. (1993) Mutational analysis of the pea phytochrome A chromophore pocket: chromophore assembly with apophytochrome A and photoreversibility, Biochemistry 32, 14165-14172.
31. Park, C. M., Kim, J. I., Yang, S. S., Kang, J. G., Kang, J. H., Shim, J. Y., Chung, Y. H., Park, Y. M., and Song, P. S. (2000) A second photochromic bacteriophytochrome from Synechocystis sp. PCC 6803: spectral analysis and down-regulation by light, Biochemistry 39, 10840-10847.
32. Li, L., Murphy, J. T., and Lagarias, J. C. (1995) Continuous fluorescence assay of phytochrome assembly in vitro, Biochemistry 34, 7923-7930.