Simultaneous detection of North American and European porcine reproductive and respiratory syndrome virus using real-time quantitative reverse transcriptase-PCR

Journal of Veterinary Diagnostic Investigation

Volumn 17, Issue 2, 2005, Pages 165-170

  Kleiboeker, Steven B ^a   Schommer, Susan K ^a   Lee, Sang Myeong ^a   Watkins, Sandy ^b   Chittick, Wayne ^b   Polson, Dale ^b  

^a UNIVERSITY OF MISSOURI   (United States)

^b BOEHRINGER INGELHEIM VETMEDICA INC   (United States)

Author keywords

Multiplex; Porcine arterivirus; Porcine reproductive and respiratory syndrome virus; Quantitative; Real time; Reverse transcriptase PCR

Indexed keywords

ARTERIVIRUS; PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS; SUIDAE; SUS SCROFA;

EID: 15944403654     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870501700211     Document Type: Article

References (26)

1. Albina E, Madec F, Cariolet R, Torrison J: 1994, Immune response and persistence of the porcine reproductive and respiratory syndrome virus in infected pigs and farm units. Vet Rec 134:567-573.
2. Allende R, Laegreid WW, Kutish GF, et al.: 2000, Porcine reproductive and respiratory syndrome virus: description of persistence in individual pigs upon experimental infection. J Virol 74:10834-10837.
3. Andreyev VG, Wesley RD, Mengeling WL, et al.: 1997, Genetic variation and phylogenetic relationships of 22 porcine reproductive and respiratory syndrome virus (PRRSV) field strains based on sequence analysis of open reading frame 5. Arch Virol 142:993-1001.
4. Christopher-Hennings J, Nelson EA, Hines RJ, et al.: 1995, Persistence of porcine reproductive and respiratory syndrome virus in serum and semen of adult boars. J Vet Diagn Invest 7:456-464.
5. Christopher-Hennings J, Nelson EA, Nelson JK, et al.: 1995, Detection of porcine reproductive and respiratory syndrome virus in boar semen by PCR. J Clin Microbiol 33:1730-1734.
6. Collins JE, Benfield DA, Christianson WT, et al.: 1992, Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Invest 4:117-126.
7. Egli C, Thur B, Liu L, Hofmann MA: 2001, Quantitative TaqMan RT-PCR for the detection and differentiation of European and North American strains of porcine reproductive and respiratory syndrome virus. J Virol Methods 98:63-75.
8. Gilbert SA, Larochelle R, Magar R, et al.: 1997, Typing of porcine reproductive and respiratory syndrome viruses by a multiplex PCR assay. J Clin Microbiol 35:264-267.
9. Guarino H, Goyal SM, Murtaugh MP, et al.: 1999, Detection of porcine reproductive and respiratory syndrome virus by reverse transcription-polymerase chain reaction using different regions of the viral genome. J Vet Diagn Invest 11:27-33.
10. Kapur V, Elam MR, Pawlovich TM, Murtaugh MP: 1996, Genetic variation in porcine reproductive and respiratory syndrome virus isolates in the Midwestern United States. J Gen Virol 77:1271-1276.
11. Kleiboeker SB: 2003, Applications of competitor RNA in diagnostic RT-PCR. J Clin Microbiol 41:2055-2061.
12. Kleiboeker SB, Lehman JR, Fangman TF: 2002, Concurrent use of reverse transcription-polymerase chain reaction testing of oropharyngeal scrapings and paired serological testing for detection of porcine reproductive and respiratory syndrome virus in sows. J Swine Health Prod 10:251-258.
13. Mackay IM, Arden KE, Nitsche A: 2002, Real-time PCR in virology. Nucleic Acids Res 30:1292-1305.
14. Meng XJ, Paul PS, Halbur PG, Morozov I: 1995, Sequence comparison of open reading frames 2 to 5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus. J Gen Virol 76:3181-3188.
15. Mengeling WL, Lager KM: 2000, A brief review of procedures and potential problems associated with the diagnosis of porcine reproductive and respiratory syndrome. Vet Res 31:61-69.
16. Oleksiewicz MB, Botner A, Madsen KG, Storgaard T: 1998, Sensitive detection and typing of porcine reproductive and respiratory syndrome virus by RT-PCR amplification of whole viral genes. Vet Microbiol 64:7-22.
17. Ropp SL, Wees CE, Fang Y, et al.: 2004, Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States. J Virol 78:3684-3703.
18. Snijder EJ, Meulenberg JJ: 1998, The molecular biology of arteriviruses. J Gen Virol 79:961-979.
19. Spagnuolo-Weaver M, Walker IW, Campbell ST, et al.: 2000, Rapid detection of porcine reproductive and respiratory syndrome viral nucleic acid in blood using a fluorimeter based PCR method. Vet Microbiol 76:15-23.
20. Spagnuolo-Weaver M, Walker IW, McNeilly F, et al.: 1998, The reverse transcription polymerase chain reaction for the diagnosis of porcine reproductive and respiratory syndrome: comparison with virus isolation and serology. Vet Microbiol 62:207-215.
21. Suarez P, Zardoya R, Prieto C, et al.: 1994, Direct detection of the porcine reproductive and respiratory syndrome (PRRS) virus by reverse polymerase chain reaction (RT-PCR). Arch Virol 135:89-99.
22. Umthun, AR, Mengeling WL: 1999, Restriction fragment length polymorphism analysis of strains for porcine reproductive and respiratory syndrome virus by use of a nested-set reverse transcription-polymerase reaction. Am J Vet Res 60:802-806.
23. Wensvoort G, Terpstra C, Pol JMA, et al.: 1991, Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q 13:121-130.
24. Wilhelm J, Pingoud A: 2003, Real-time polymerase chain reaction. Chembiochem 4:1120-1128.
25. Wills RW, Zimmerman JJ, Yoon KJ, et al.: 1997, PRRS virus: a persistent infection. Vet Microbiol 55:231-240.
26. Yaeger MJ: 2002, The diagnostic sensitivity of immunohistochemistry for the detection of porcine reproductive and respiratory syndrome virus in the lung of vaccinated and unvaccinated swine. J Vet Diagn Invest 14:15-19.