Molecular diagnosis of synovial sarcoma: RT-PCR detection of SYT-SSX1/2 fusion transcripts in paraffin-embedded tissue

Medical Science Monitor

Volumn 11, Issue 3, 2005, Pages

  Tvrdík, Daniel ^a   Povýšil, Ctibor ^a,b   Svatošová, Jana ^a,b   Dundr, Pavel ^a,b  

^a CHARLES UNIVERSITY   (Czech Republic)

^b CHARLES UNIVERSITY   (Czech Republic)

Author keywords

Chromosomal translocation; Paraffin embedded tissue; RT PCR; Synovial sarcoma; SYT SSX

Indexed keywords

PARAFFIN; RNA;

ADULT; AGED; ARTICLE; CHROMOSOME 11P; CHROMOSOME 11Q; CHROMOSOME TRANSLOCATION; CLINICAL ARTICLE; DIAGNOSTIC PROCEDURE; FEMALE; GENE; GENE AMPLIFICATION; GENE EXPRESSION; GENE FUSION; HISTOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MALE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPINDLE CELL CARCINOMA; SSX1 GENE; SSX2 GENE; SYNOVIAL SARCOMA; SYT GENE;

ADULT; AGED; CHROMOSOMES, HUMAN, PAIR 18; CHROMOSOMES, HUMAN, X; CZECH REPUBLIC; FEMALE; HUMANS; MALE; MIDDLE AGED; NEOPLASM PROTEINS; ONCOGENE PROTEINS, FUSION; PARAFFIN EMBEDDING; RETROSPECTIVE STUDIES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SARCOMA, SYNOVIAL; TRANSCRIPTION, GENETIC; TRANSLOCATION, GENETIC; TUMOR MARKERS, BIOLOGICAL;

EID: 15944401352     PISSN: 12341010     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. Presant CA, Russel WO, Alexander RW, Fu YS: Soft-tissue and bone sarcoma histopathology peer review: the frequency of disagreement in diagnosis and the need for second pathology opinions. J Clin Oncol 1986; 4: 1658-61
2. Dickersin GR: Synovial sarcoma: a review and update, with emphasis on the ultrastructural characterization of the nonglandular component. Ultrastruct Pathol, 1991; 15: 379-402
3. Pappo AS, Fontanesi J, Luo X: Synovial sarcoma in children and adolescents. J Clin Oncol, 1994; 12: 2360-66
4. Bednář B, Povýšil C: Soft tissue tumors (review of differential diagnosis). Cesk Patol, 1986; 22: 177-91
5. Povýšil C: Synovial sarcoma with squamous metaplasia. Ultrastruct Pathol, 1984; 7: 207-13
6. Gaertner E, Zeren EH, Fleming MV et al: Biphasic synovial sarcomas arising in the pleural cavity. Am J Surg Pathol, 1996; 20: 36-40
7. Kaplan MA, Goodman D, Satish J et al: Primary pulmonary sarcoma with morphologic features of monophasic synovial sarcoma and chromosome translocation t(X;18). Am J Clin Pathol, 1996; 105: 195-99
8. Sandberg A, Turc-Carel C: The cytogenetics of solid tumours: relation to diagnosis, classification and pathology. Cancer, 1987; 59: 387-95
9. Turc-Carel C, Dal Cin P, Limon J et al: Involvement of chromosome X in primary cytogenetic change in human neoplasia: nonrandom translocarion in synovial sarcoma. Proc Natl Acad Sci USA, 1987; 84: 1981-85
10. dark J, Rocques PJ, Crew AJ et al: Identification of novel genes, SYT and SSX, involved in the t(X;18)(p11.2;q11.2) translocation found in human synovial sarcoma. Nat Genet, 1994; 7: 502-8
11. de Leeuw B, Balemans M, Olde Weghuis D, Geurts van Kessel A: Identification of two alternative fusion genes, SYT-SSX1 and SYT-SSX2, in t(X;18)(p11.2;q11.2)-positive synovial sarcomas. Hum Mol Genet, 1995; 4: 1097-99
12. Crew AJ, Clark J, Fisher C et al: Fusion of SYT to two genes, SSX1 and SSX2, encoding proteins with homology to the Kruppel-associated box in human synovial sarcoma. EMBO J, 1995; 14: 2333-40
13. Witzgall R, O'Leary E, Leaf A et al: The Kruppel-associated box-A (KRABA) domain of zinc finger proteins mediates transcriptional repression. Proc Natl Acad Sci USA, 1994; 91: 4514-18
14. Tureci O, Sahin U, Schobert I et al: The SSX-2 gene, which is involved in the t(X;18) translocation of synovial sarcomas, codes the human tumor antigen HOM-MEL-40. Cancer Res, 1996; 56: 4766-72
15. de Leeuw B, Balemans M, Geurts van Kessel A: A novel Kruppel-associated box containing the SSX gene (SSX3) on the human X chromosome is not implicated in t(X;18)-positive synovial sarcomas. Cytogenet Cell Genet, 1996; 73: 179-83
16. Bruijn DRH, Baats E, Zechner U et al: Isolation and characterisation of the mouse homolog of SYT, a gene implicated in the development of human synovial sarcomas. Oncogene, 1996; 13: 643-48
17. Rabbitts TH: Chromosomal translocation in human cancer. Nature, 1994; 372: 143-49
18. Brett D, Whitehouse S, Antonson P et al: The SYT protein involved in the t(X;18) synovial sarcoma translocation is a transcriptional activator localized in nuclear bodies. Hum Mol Genet, 1997; 6: 1559-64
19. dos Santos NR, de Bruijn DRH, Balemans M et al: Nuclear localization of SYT, SSX and the synovial associated SYT-SSX fusion proteins. Hum Mol Genet, 1997; 6: 1549-58
20. Kawai A, Woodruff J, Healey JH et al: SYT-SSX gene fusion as a determinant of morphology and prognosis in Synovial sarcoma. N Engl J Med, 1998; 338: 153-60
21. Willeke F, Mechtersheimer G, Schwarzbach M et al: Detection of SYT-SSX1/2 fusion transcripts by reverse transcriptase-Polymerase chain reaction (RT-PCR) is a valuable diagnostic tool in Synovial sarcoma. Eur J Cancer, 1998; 34: 2087-93
22. Argani P, Zakowski MF, Klimstra DS et al: Detection of SYT-SSX chimeric RNA of synovial sarcoma in paraffin-embedded tissue and its application in problematic cases. Mod Pathol, 1998; 11: 65-71
23. Weiss SW: WHO: Histological typing of soft tissue tumors. Berlin: Springer, 1994
24. Povýšil C, Bennett R, Povýšilova V: CD68 positivity of the so-called meconium corpuscles in human foetal intestine. Cesk Patol, 2001; 37: 7-9
25. nd edition. New York: John Wiley, 1992
26. Prejzner W: Relationship of the BCR gene breakpoint and the type of BCR/ABL transcript to clinical course, prognostic indexes and survival in patients with chronic myeloid leukemia. Med Sci Monit, 2002; 8(5): CR193-CR197
27. Niller HH, Salamon D, Ilg K et al: The in vivo binding site for onco-protein c-Myc in the promoter for Epstein-Barr virus (EBV) encoding RNA (EBER) 1 suggests a specific role for EBV in lymphomagenesis. Med Sci Monit, 2003; 9(1): HV1-HY9
28. rd edition. New York: Garland Publishing, 1994
29. Erikson J, ar-Rushdi A, Drwinga HL et al: Transcriptional activation of the translocated c-myc oncogene in Burkitt lymphoma. Proc Natl Acad Sci USA, 1983; 80: 820-24
30. Djaborkhel R, Tvrdík D, Eckschlager T et al: Cyclin A down-regulation in TGFbetal-arrested follicular lymphoma cells. Exp Cell Res, 2000; 261: 250-59
31. WAF1 in growth-arrested lymphoma cells. J Struct Biol, 2002; 140: 49-56
32. Prejzner W: Relationship of the BCR gene breakpoint and the type of BCR/ABL transcript to clinical course, prognostic indexes and survival in patients with chronic myeloid leukemia. Med Sci Monit, 2002; 8: CR193-CR197
33. Tvrdík D: Effect of TGFbetal on the expression and phosphorylation of key regulators of the cell cycle in malignant B cells. Med Sci Monit, 2004; 10(12): BR447-R454