α1-Adrenoceptors stimulate a Gαs protein and reduce the transient outward K+ current via a cAMP/PKA-mediated pathway in the rat heart

American Journal of Physiology - Cell Physiology

Volumn 288, Issue 3 57-3, 2005, Pages

  Gallego, Mónica ^a   Setién, Raúl ^a,b   Puebla, Lilian ^b   Boyano Adánez, María Del Carmen ^b   Arilla, Eduardo ^b   Casis, Oscar ^a  

^a UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

^b UNIVERSITY OF ALCALÁ   (Spain)

Author keywords

Compartmentalization; Potassium currents

Indexed keywords

ADENYLATE CYCLASE; ALPHA ADRENERGIC RECEPTOR; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; PHENYLEPHRINE; PHOSPHOLIPASE C; PROTEIN KINASE C; ALPHA 1 ADRENERGIC RECEPTOR; ALPHA ADRENERGIC RECEPTOR STIMULATING AGENT; CALMODULIN DEPENDENT PROTEIN KINASE II; CALMODULIN-DEPENDENT PROTEIN KINASE II; DRUG DERIVATIVE; FORSKOLIN; NORADRENALIN; POTASSIUM; POTASSIUM CHANNEL; PROTEIN KINASE (CALCIUM,CALMODULIN); STIMULATORY GUANINE NUCLEOTIDE BINDING PROTEIN;

ANIMAL CELL; ARTICLE; CELL COMPARTMENTALIZATION; CELL MEMBRANE; CONTROLLED STUDY; CYTOSKELETON; ENZYME ACTIVATION; HEART MUSCLE CELL; NONHUMAN; PHOSPHORYLATION; POTASSIUM CURRENT; PRIORITY JOURNAL; RAT; SIGNAL TRANSDUCTION; ANIMAL; CELL CULTURE; CYTOLOGY; DRUG EFFECT; HEART MUSCLE; METABOLISM; PATCH CLAMP; PHYSIOLOGY; SPRAGUE DAWLEY RAT;

ADRENERGIC ALPHA-AGONISTS; ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CELL MEMBRANE; CELLS, CULTURED; CYCLIC AMP; CYCLIC AMP-DEPENDENT PROTEIN KINASES; FORSKOLIN; GTP-BINDING PROTEIN ALPHA SUBUNITS, GS; MYOCARDIUM; MYOCYTES, CARDIAC; NOREPINEPHRINE; PATCH-CLAMP TECHNIQUES; PHENYLEPHRINE; PHOSPHOLIPASE C; POTASSIUM; POTASSIUM CHANNELS; RATS; RATS, SPRAGUE-DAWLEY; RECEPTORS, ADRENERGIC, ALPHA-1; SIGNAL TRANSDUCTION;

EID: 15944383449     PISSN: 03636143     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajpcell.00124.2004     Document Type: Article

References (48)

1. Adams JP, Anderson AE, Varga AW, Dineley KT, Cook RG, Pfaffinger PJ, and Sweatt JD. The A-type potassium channel Kv4.2 is a substrate for the mitogen-activated protein kinase ERK. J Neurochem 75: 2277-2287, 2000.
2. Amos GJ, Wettwer E, Metzger F, Li Q, Himmel HM, and Ravens U. Differences between outward currents of human atrial and subepicardial ventricular myocytes. J Physiol 491: 31-50, 1996.
3. Anderson AE, Adams JP, Qian Y, Cook RG, Pfaffinger PJ, and Sweatt JD. Kv4.2 phosphorylation by cyclic AMP-dependent protein kinase. J Biol Chem 275: 5337-5346, 2000.
4. + currents in rat ventricular myocytes. Proc Natl Acad Sci USA 85: 8756-8760, 1988.
5. o. J Biol Chem 268: 7532-7537, 1993.
6. Bogoyevitch MA, Parker PJ, and Sugden PH. Characterization of protein kinase C isotype expression in adult rat heart: protein kinase C-ε is a major isotype present, and it is activated by phorbol esters, epinephrine, and endothelin. Circ Res 72: 757-767, 1993.
7. Bradford MM. A rapid and sensitive method for the quantitation of microgram of protein utilizing the principle of protein-dye binding. Anal Biochem 105: 248-254, 1976.
8. 1-adrenergic modulation of the transient outward current in rabbit atrial myocytes. J Physiol 431: 689-712, 1990.
9. 1-Adrenergic and muscarinic cholinergic stimulation of phosphoinositide hydrolysis in adult rat cardiomyocytes. Circ Res 57: 532-537, 1985.
10. Buxton ILO and Brunton LL. α-Adrenergic receptors on rat ventricular myocytes: characteristics and linkage to cAMP metabolism. Am J Physiol Heart Circ Physiol 251: H307-H313, 1986.
11. to and Kv4.2/43 expressed in HEK cells (Abstract). Biophys J 84: 414A, 2003.
12. + current densities in rat ventricle. Life Sci 63: 391-400, 1998.
13. + channel in ventricular muscle: a molecular correlate for the transient outward current. Circ Res 79: 659-668, 1996.
14. 1-Adrenergic modulation of the transient outward current in rabbit atrial myocytes. J Physiol 423: 257-277, 1990.
15. Freissmuth M, Boehm S, Beindl W, Nickel P, Ijzerman AP, Hohenegger M, and Nanoff C. Suramin analogues as subtype-selective G protein inhibitors. Mol Pharmacol 49: 602-611, 1996.
16. 1-adrenergic signal within caveolae: caveolin expression and the development of cardiac hypertrophy. Cardiovasc Res 51: 709-716, 2001.
17. Gallego M, Casis E, Izquierdo MJ, and Casis O. Restoration of cardiac transient outward potassium current by norepinephrine in diabetic rats. Pflügers Arch 441: 102-107, 2000.
18. Gallego M and Casis O. Regulation of cardiac transient outward potassium current by norepinephrine in normal and diabetic rats. Diabetes Metab Res Rev 17: 304-309, 2001.
19. Gilman AG. A protein binding assay for adenosine 3′5′-cyclic monophosphate. Proc Natl Acad Sci USA 67: 305-312, 1970.
20. 1-adrenoceptor subtype-mediated responses in Fisher 344 rat aorta. Mol Pharmacol 52: 1064-1070, 1997.
21. Hamill OP, Marty A, Neher E, Sakmann B, and Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell free membrane patches. Pflüg ers Arch 391: 85-100, 1981.
22. + channels in dendrites of hippocampal CA1 pyramidal neurons by activation of PKA and PKC. J Neurosci 18: 3521-3528, 1998.
23. to in rat ventricular myocytes. Br J Pharmacol 129: 1113-1120, 2000.
24. Horie K, Itoh H, and Tsujimoto G. Hamster alpha 1B-adrenergic receptor directly activates Gs in the transfected Chinese hamster ovary cells. Mol Pharmacol 48: 392-400, 1995.
25. Isenberg G and Klöckner U. Calcium tolerant ventricular myocytes prepared by preincubation in a "KB medium." Pflügers Arch 395: 6-18, 1982.
26. Jelsema CL and Axelrod J. Stimulation of phospholipase A2 activity in bovine rod outer segments by the beta gamma subunits of transducin and its inhibition by the alpha subunit. Proc Natl Acad Sci USA 84: 3623-3627, 1987.
27. i. Circ Res 87: 705-709, 2000.
28. Kochs G, Hummel R, Meyer D, Hug H, Marmé D, and Sarre TF. Activation and substrate specificity of the human protein kinase C α and ζ isoenzymes. Eur J Biochem 216: 597-606, 1993.
29. 1A-adrenoceptors in rat ventricular myocytes. J Cardiovasc Pharmacol 35: 212-218, 2000.
30. Martiny-Baron G, Kazanietz MG, Mischak H, Blumberg PM, Kochs G, Hug H, Marmé D, and Schächtele C. Selective inhibition of protein kinase C isozymes by the indolocarbazole Gö 6976. J Biol Chem 268: 9194-9197, 1993.
31. 2+/calmodulin dependent protein kinase II phosphorylation site in vascular calpain II. J Biochem (Tokyo) 115: 41-46, 1994.
32. + current, by PKC. Am J Physiol Heart Circ Physiol 273: H1775-H1786, 1997.
33. Nakayama T and Fozzard HA. Adrenergic modulation of the transient outward current in isolated canine Purkinje cells. Circ Res 62: 162-172, 1988.
34. Nishizuka Y. The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature 334: 661-665, 1988.
35. to via PKA-dependent phosphorylation. Am J Physiol Heart Circ Physiol 272: H1791-H1797, 1997.
36. Po SS, Wu RC, Juang GJ, Kong W, and Tomaselli GF. Mechanism of α-adrenergic regulation of expressed hKv4.3 currents. Am J Physiol Heart Circ Physiol 281: H2518-H2527, 2001.
37. Ravens U, Wang XL, and Wettwer E. α Adrenoceptor stimulation reduces outward currents in rat ventricular myocytes. J Pharmacol Exp Ther 250: 364-370, 1989.
38. Roberds SL, Knoth KM, Po S, Blair TA, Bennett PB, Hartshorne RP, Snyders DJ, and Tamkun MM. Molecular biology of the voltage-gated potassium channels of the cardiovascular system. J Cardiovasc Electrophysiol 4: 68-80, 1993.
39. Rosati B, Pan Z, Lypen S, Wang HS, Cohen I, Dixon JE, and McKinnon D. Regulation of KChIP2 potassium channel β subunit gene expression underlies the gradient of transient outward current in canine and human ventricle. J Physiol 533: 119-125, 2001.
40. αo. FEBS Lett 428: 291-294, 1998.
41. + currents in rat ventricular myocytes and its modification by hormonal status. J Physiol 520: 439-449, 1999.
42. Steinberg SF and Brunton LL. Compartmentation of G protein-coupled signaling pathways in cardiac myocytes. Annu Rev Pharmacol Toxicol 41: 751-773, 2001.
43. 1-adrenoceptors on transient outward current in isolated rat ventricular cells. Pflügers Arch 415: 575-581, 1990.
44. to channels: Kv4.2, Kv4.3, and other possibilities? Cardiovasc Res 41: 16-18, 1999.
45. + currents via different signaling pathways in canine ventricular myocytes. J Biol Chem 276: 40811-40816, 2001.
46. Wettwer E, Amos G, Gath J, Zerkowski H, Reidemeister J, and Ravens U. Transient outward current in human and rat ventricular myocytes. Cardiovasc Res 27: 1662-1669, 1993.
47. i proteins and its physiological relevance in murine cardiac myocytes. Circ Res 84: 43-52, 1999.
48. 2-adrenoceptor to a pertussis toxin-sensitive G protein in rat cardiac myocytes. Mol Pharmacol 47: 322-329, 1995.