Effect of Helicobacter pylori on gastric epithelial cell migration and proliferation in vitro: Role of VacA and CagA

Infection and Immunity

Volumn 64, Issue 7, 1996, Pages 2829-2833

  Ricci, Vittorio ^a   Ciacci, Carolina ^b   Zarrilli, Raffaele ^c   Sommi, Patrizia ^a   Tummuru, Murali K R ^d   Del Vecchio Blanco, Camillo ^e   Bruni, Carmelo B ^c   Cover, Timothy L ^d   Blaser, Martin J ^d   Romano, Marco ^d,e  

^a UNIVERSITY OF PAVIA   (Italy)

^b Granelli III Piano   (Italy)

^c Dipartimento di Biologia   (Italy)

^d UNIVERSITY OF NAPLES FEDERICO II   (Italy)

^e VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

UNCLASSIFIED DRUG; VACUOLATING CYTOTOXIN VAC A; VIRULENCE FACTOR; VIRULENCE FACTOR CAG A;

ARTICLE; BACTERIAL IMMUNITY; BACTERIAL VIRULENCE; BACTERIUM CULTURE; CELL MIGRATION; CELL PROLIFERATION; CONTROLLED STUDY; GROWTH REGULATION; HELICOBACTER PYLORI; HUMAN; HUMAN CELL; PRIORITY JOURNAL; STOMACH EPITHELIUM; STOMACH MUCOSA LESION;

EID: 8944232864     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.64.7.2829-2833.1996     Document Type: Article

References (46)

1. Barnard, J. A., R. D. Beauchamp, W. E. Russell, R. N. Dubois, and R. J. Coffey. 1995. Epidermal growth factor-related peptides and their relevance to gastrointestinal pathophysiology. Gastroenterology 108564-580.
2. Blaser, M. J. 1992. Hypotheses on the pathogenesis and natural history of Helicobacter pylori-induced inflammation. Gastroenterology 102720-727.
3. Blaser, M. J., G. I. Perez-Perez, H. Kleanthous, T. L. Cover, R. M. Peek, P.-H. Chyou, G. N. Stemmermann, and A. Nomura. 1995. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res. 552111-2115.
4. Chang, K., Y. Fujiwara, F. Wyle, and A. Tarnawski. 1993. Helicobacter pylori toxin inhibits growth and proliferation of cultured gastric cells Kalo III. Gastroenterology 104A52.
5. Chang, K., F. Wyle, J. Stachura, and A. Tarnawski. 1993. Helicobacter pylori eytotoxin delays healing of experimental gastric ulcers and inhibits cell proliferation at the ulcer margin a key to its mechanism of action? Gastroenterology 104A52.
6. Ciacci, C, S. E. Lind, and D. K. Podolsky. 1993. Transforming growth factor beta regulation of migration in wounded rat intestinal epithelial monolayers. Gastroenterology 10593-101.
7. Covacci, A., S. Censini, M. Bugnoli, R. Petracca, D. Burroni, G. Macchia, A. Massone, E. Papini, Z. Xiang, N. Figura, and R. Rappuoli. 1993. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc. Natl. Acad. Sci. USA 905791-5795.
8. Cover, T. L., and M. J. Blaser. 1992. Purification and characterization of the vacuolating toxin from Helicobacter pylori. J. Biol. Chem. 26710570-10575.
9. Cover, T. L., C. P. Dooley, and M. J. Blaser. 1990. Characterization and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect. Immun. 58603-610.
10. + Helicobacter pylori strains. J. Clin. Microbiol. 331495-1500.
11. Cover, T. L., M. K. R. Tummuru, P. Cao, S. A. Thompson, and M. J. Blaser. 1994. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J. Biol. Chem. 26910566-10573.
12. Dignass, A. U., and D. K. Podolsky. 1993. Cytokine modulation of intestinal epithelial cell restitution central role of transforming growth factor beta. Gastroenterology 1051323-1332.
13. Dunn, B. E. 1993. Pathogenic mechanisms of Helicobacter pylori. Gastroenterol. Clin. N. Am. 2243-57.
14. Enoch, T., and C. Norbnry. 1995. Cellular responses to DNA damage cell-cycle checkpoints, apoptosis and the roles of p53 and ATM. Trends Biochem. Sci. 20426-430.
15. Fiocca, R., O. Luinetti, L. Villani, A. M. Chiaravalli, C. Capella, and E. Solcia. 1994. Epithelial cytotoxicity, immune responses, and inflammatory components of Helicobacter pylori gastritis. Scand. J. Gastroenterol. 29 (Suppl. 205)11-21.
16. Fujiwara, Y., F. Wyle, T. Arakawa, K. Kobayashi, and A. Tarnawski. 1995. H.p. toxin reduces EGF binding to its receptor and proliferative response of human gastric Kato III cells to EGF stimulation. Important mechanism for H.p. interference with the ulcer healing? Gastroenterology 108A97.
17. Ghiara, P., M. Marchetti, M. J. Blaser, M. K. R. Tummuru, T. L. Cover, E. D. Segal, L. S. Tompkins, and R. Rappuoli. 1995. Role of the Helicobacter pylori virulence factors vacuolating cytotoxin, CagA, and ureasc in a mouse model of disease. Infect. Immun. 634154-4160.
18. Hojo, H. 1977. Establishment of cultured lines of human stomach cancer. Origin and their morphological characteristics. Niigata Igakukai Zasshi 91 737-752.
19. Ito, S. 1987. Functional gastric morphology, p. 817-851. In L. R. Johnson (ed.), Physiology of the gastrointestinal tract, 2nd ed. Raven Press, New York.
20. Johnson, L. R. 1988. Regulation of gastrointestinal mucosal growth. Physiol. Rev. 68456-502.
21. Konturek, I. W., T. Brzozowski, and S. J. Konturek. 1991. Epidermal growth factor in protection, repair, and healing of gastroduodenal mucosa. J. Clin. Gastroenterol. 13(Suppl. 1)S88-S97.
22. Lee, A., J, Fox, and S. Hazell. 1993. Pathogenicity of Helicobacter pylon a perspective. Infect. Immun. 611601-1610.
23. Leung, K. M., P. K. Hui, W. Y. Chan, and T. M. M. Thomas. 1992. Helicobacter pylori-related gastritis and gastric ulcer. Anat. Pathol. 98569-574.
24. Levi, S., K. Beardshall, G. Haddad, R. Playford, P. Ghosh, and J. Calam. 1989. Campylobacter pylori and duodenal ulcers the gastrin link. Lancet 11167-1168.
25. Lynch, D. A. F., N. P. Mapstone, A. M. T. Clarke, G. M. Sobala, P. Jackson, L. Morrison, M. F. Dixon, P. Quirke, and A. T. R. Axon. 1995. Cell proliferation in Helicobacter pylori associated gastritis and the effect of eradication therapy. Gut 36346-350.
26. Mikami, H., S. Watanabe, M. Hirose, and N. Sato. 1994. Role of extracellular matrix in wound repair by cultured gastric mucosal cells. Biochem. Biophys. Res. Commun. 202285-292.
27. Moss, S. F., J. Calam, B. Agarwal, S. Wang, and P. R. Holt. 1995. Helicobacter pylori infection induces gastric epithelial apoptosis in vivo. Gastroenterology 108A171.
28. NIH Consensus Development Panel. 1994. Helicobacter pylori in peptic ulcer disease. JAMA 27265-69.
29. Nomura, A., G. N. Stemmermann, P.-H. Chyou, I. Kalo, G. I. Perez-Perez, and M. J. Blaser. 1991. Helicobacter pylori infection and gastric carcinoma in a population of Japanese Americans in Hawaii. N. Engl. J. Med. 3251132-1136.
30. Parsonnet, J., G. D. Friedman, D. P. Vandersteen, Y. Chang, J. H. Vogelman, N. Orentreich, and R. K. Sibley. 1991. Helicobacter pylori infection and the risk of gastric carcinoma. N. Engl. J. Med. 3251127-1131.
31. Ricci, V., R. Fiocca, P. Sommi, E. Cova, M. Romano, O. Luinetti, P. Barattini, K. J. Ivey, E. Solcia, and U. Ventura. 1992. MKN 28 cell line a useful tool to study human gastric epithelial cells. Pflüg. Arch. 420R182.
32. Ricci, V., P. Sommi, R. Fiocca, E. Cova, N. Figura, M. Romano, K. J. Ivey, E. Solcia, and U. Ventura. 1993. Cytotoxicity of Helicobacter pylori on human gastric epithelial cells in vitro role of cytotoxin(s) and ammonia. Eur. J. Gastroenterol. Hepatol. 5687-694.
33. Romano, M., W. H. Polk, J. A. Awad, C. L. Arteaga, L. B. Nanney, M. J. Wargovich, E. R. Kraus, C. R. Boland, and R. J. Coffey. 1992. Transforming growth factor alpha protection against drug-induced injury to the rat gastric mucosa in vivo. J. Clin. Invest. 902409-2421.
34. Romano, M., M. Razandi, S. Sekhon, W. J. Krause, and K. J. Ivey. 1988. Human cell line for study of damage to gastric epithelial cells in vitro. J. Lab. Clin. Med. 111430-440.
35. Sharma, S. A., M. K. R. Tummuru, G. G. Miller, and M. J. Blaser. 1995. Interleukin-8 response of gastric epithelial cell lines to Helicobacter pylori stimulation in vitro. Infect. Immun. 631681-1687.
36. Silen, W. 1987. Gastric mucosal defense and repair, p. 1055-1069. In L. R. Johnson (ed.). Physiology of the gastrointestinal tract, 2nd ed. Raven Press, New York.
37. Snedecor, G. W., and W. G. Cockran. 1967. Statistical methods. Iowa State University Press, Ames.
38. Telford, J. L., A. Covacci, P. Ghiara, C. Montecucco, and R. Rappuoli. 1994. Unravelling the pathogenic role of Helicobacter pylori in peptic ulcer potential new therapies and vaccines. Trends Biotechnol. 12420-426.
39. Telford, J. L., P. Ghiara, M. Dell'Orco, M. Comanducci, D. Burroni, M. Bugnoli, M. F. Tecce, S. Censini, A. Covacci, Z. Xiang, E. Papini, C. Montecucco, L. Parente, and R. Rappuoli. 1994. Gene structure of the Helicobacter pylori cytotoxin and evidence of its key role in gastric disease. J. Exp. Med. 1791653-1658.
40. Tracz, P., S. Gustavsson, and A. Uribe. 1995. Cell proliferation in the gastrointestinal epithelium of patients with duodenal ulcer and Helicobacter pylori infection. Gastroenterology 108A243.
41. Tsujii, M., S. Kawano, S. Tsuji, T. Ito, K. Nagano, Y. Sasaki, N. Hayashi, H. Fusamoto, and T. Kamada. 1993. Cell kinetics of mucosal atrophy in rat stomach induced by long-term administration of ammonia, Gastroenterology 104796-801.
42. Tummuru, M. K. R., T. L. Cover, and M. J. Blaser. 1993. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori evidence of linkage to cytotoxin production. Infect. Immun. 611799-1809.
43. Tummuru, M. K. R., T. L. Cover, and M. J. Blaser. 1994. Mutation of the cytotoxin-associated cagA gene does not affect the vacuolating cytotoxin activity of Helicobacter pylori. Infect. Immun. 622609-2613.
44. Wallace, J. L. 1990. Endogenous mediators of mucosal injury and protection. Eur. J. Gastroenterol. Hepatol. 2186-188.
45. World Health Organization. 1994. Schistosomes, liver flukes and Helicobacter pylori. IARC monographs on the evaluation of carcinogenic risks to humans. 61177-240.
46. Xiang, Z., S. Censini, P. F. Bayeli, J. L. Telford, N. Figura, R. Rappuoli, and A. Covacci. 1995. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major type and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect. Immun. 6394-98.