Scale-up of processes to isolate the misstargeted rBm86 protein from Pichia pastoris

African Journal of Biotechnology

Volumn 3, Issue 11, 2004, Pages 599-605

  Buxadó, Jose A ^a   Heynngnezz, Lázaro E ^a   Juiz, Arturo G ^a   Tamayo, Grisel ^a   Lima, Ivonne R ^a   Marshalleck, Hubert D ^a   Mola, Ernesto L ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

Bm86 protein; Downstream process; Pichia pastoris; Scale up; Vaccine

Indexed keywords

MEMBRANE PROTEIN; PROTEIN BM86; RECOMBINANT PROTEIN; RECOMBINANT VACCINE; UNCLASSIFIED DRUG;

ARTICLE; CELL DISRUPTION; CONTROLLED STUDY; DIAFILTRATION; DOWNSTREAM PROCESSING; EXTRACTION; FUNGUS ISOLATION; INSECT CONTROL; METHODOLOGY; NONHUMAN; PICHIA PASTORIS; PROTEIN ISOLATION; PROTEIN TARGETING; SCALE UP; SOLUBILIZATION; STATISTICAL ANALYSIS; ULTRAFILTRATION;

ACARI; BOOPHILUS MICROPLUS; BOS TAURUS; PICHIA PASTORIS;

EID: 15544375999     PISSN: 16845315     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Boué O, Sánchez K, Tamayo G, Hernández L, Reytor E, Enríquez A (1997). Single-step purification of recombinant Bm86 protein produced in Pichia pastoris by salting-out and by acid precipitation of contaminants. Biotechnol. Techniq. 13:561-565.
2. Boue O, Farnós O, González A, Fernandez R, Acosta JA, Valdés R, González LJ, Guanche Y, Izquierdo G, Suárez M, Domínguez I, Machado H, Rodríguez M, Lleonart R (2004). Production and biochemical characterization of the recombinant Boophilus microplus Bm95 antigen from Pichia pastoris. Exp. Appl. Acarol. 32:119-128.
3. Box GEP (1954). Evolutionary operation: A method of increasing industrial productivity. Appl. Stat. 6:3-22.
4. Box GEP (1962). Chanmugan J, Adaptive optimization of continuous processes. Ind. Eng. Chem. Fundamentals 1:2-16.
5. Bradford MM (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
6. Buckholz RG, Gleeson MAG (1991). Yeast system for the commercial production of heterologous proteins. Biotechnology 9:1067-1072.
7. Canales M, Enriquez A, Ramos E, Cabrera 0, Dandie H, Soto A et al (1997). Large-scale production in Pichia pasioris of the recombinant vaccine Gavac™ against cattle tick. Vaccine 15: 414-432.
8. Canales M, Buxadó JA, Heynngnezz L, Enriquez A (1998). Mechanical disruption of Pichia pasioris yeast to recover the recombinant glycoprotein Bm86. Enz. Microb. Technol. 23: 58-63.
9. Chanmugan J, Jenskins GM (1963). Optimum experimentation in the process industries. Chem. Eng. Progr. Symp. Ser. 42:59, 108.
10. Cregg JM, Vedvick TS, Raschke WC (1993). Recent advances in the expression of foreign genes in Pichia pasioris. Biotechnololgy 11:90-91.
11. De la Fuente J, Rodriguez M, Redondo M, Montero Co Garcia-Garcia JC, Mendez L, et al (1997). Field studies and cost-effectiveness analysis of vaccination with Gavac™ against the cattle tick Boophilus microplus. Vaccine 16:366-373.
12. Faber KN, Harder W, Geert A, Veenhuis M (1995). Methylotrophic yeast as factories for the production of foreign proteins. Yeast 11:1331-1344.
13. *Gershon M (1991). Statistical process control for the pharmaceutical industry. J. Parenteral Sci. Technol. 45:1.
14. Liu H, Tan X, Russell, KA, Veenhuis M, Cregg JM (1995). PER3, a gene required for peroxisome biogenesis in Pichia pastoris, encodes a peroxisomal membrane protein involved in protein import. J. Biol. Chem. 270:10940-10951.
15. Kurnik RT, Yu AW, Blank GS, Burton AR, Smith 0, Athalye AM, van Reis R (1995). Buffer exchange using size exclusion chromatography, counter current, dialysis, and tangential flow filtration: models, development, and industrial application. Biotechnol. Bioeng. 45:149-157.
16. Laemli, UK (1970). Claevage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
17. Paez R, Agraz A, Herrera L (1993). The recovery of the hepatitis B virus surface antigen (HBsAg) from a recombinant P. pasioris strain. Disruption and precipitation studies. Acta Biotechnol. 13:117-122.
18. Rand K, Moore T, Sriskantia A, Spring K, Tellam R, Willadsen P, et al (1989).Cloning and expression of a protective antigen from the cattle tick Boophilus microplus. Proc. Natl. Acad. Sci. USA. 86:9657.
19. Richardson MA, Smith ORJ, Kemp OH, Tellam RL (1993). Native and baculovirus-expressed forms of the immuno-protective protein Bm86 from Boophilus microplus are anchored to the cell membrane by glycosyl-phophatidyl inositol linkage.Insect. Mol. Biol. 1:139-147.
20. Rodriguez M, Rubiera R, Montesinos R, Cremata JA, Bringa R, Falcon V, et al (1994). High level expression of the B. microplus Bm86 antigen in the yeast P. pastoris forming highly immunogenic particles for cattle. J. Biotechnol. 33:135-146.
21. Rodriguez M, Penichet ML, Mouriz AE, Lavarta V, Luaces LL, Rubiera, R (1995). Control of B. microplus populations in grazing cattle vaccinated with the recombinant Bm86 antigen preparation. Veter. Parasitol. 57:339-349.
22. Tellam RL, Smith 0, Kemp OH, Willadsen P (1992).Vaccination against ticks. In:Yong WK (ed). Animal Parasite Control Utilizing Biotechnology. London: CRC Press, United Kingdom, pp. 303-330.
23. Turnbull IF, Smith ORJ, Sharp PJ, Cobon GS, Hynes MJ (1990).Expression and secretion in Aspergillus niger of a cell surface glycoprotein from the cattle tick, Boophilus microplus, by using the fungal amds promoter system. Appl. Environ. Microbiol. 56:2847-2852.
24. Waterham HR, de Vries Y, Russell K A, Xie W, Veenhuis M, Cregg JM (1996). The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1.Mol. Cell Biol. 16:2527-2536.
25. Willadsen P, Riding G, McKenna R, Kemp OH. Tellam R, Nielsen IN, et al (1989).Immunologic control of a parasitic arthropod: identification of a protective antigen from Boophilus microplus. J. Immunol. 143:1346-1351.
26. Willadsen P, Bird P, Cobon GS, Hungerford J (1995). Commercialization of a recombinant vaccine against Boophilus microplus. Parasitology 110:S43-S50.
27. Young V, Gonzalez ME, Cremata J, Montesino R, Grillo JM, Silva A, et al (1991). Methods for the expression of heterologous genes in the yeast Pichia pasioris, expression vectors and transformed microorganisms. European Patent EP 0438200 A1.