Single-step purification of recombinant Bm86 protein produced in pichia pastoris by salting-out and by acid precipitation of contaminants

Biotechnology Techniques

Volumn 11, Issue 8, 1997, Pages 561-565

  Boué, Oscar ^a   Sánchez, Karina ^a   Tamayo, Grisel ^a   Hernández, Laura ^a   Reytor, Edel ^a   Enríquez, Antonio ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

[No Author keywords available]

Indexed keywords

PICHIA PASTORIS;

EID: 0030872754     PISSN: 0951208X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018438805811     Document Type: Article

References (10)

1. Canales, M., Enríquez, A., Ramos, E., Cabrera, D., Dandie, H., Soto, A., Falcón, V., Rodríguez, M. and de la Fuente, J. (1997). Vaccine 15, 414-432.
2. Enríquez, A., Canales, M., Ramos, E., Dandie, H., Boué, O., Soto, A. and Cabrera, D. (1995) Production of a recombinant vaccine against Boophilus microplus. In: de la Fuente, J. (Ed.), Recombinant Vaccines for the Control of Cattle Tick, Elfos Scientiae, Havana, Cuba, pp 79-102.
3. Johnston, L. A. Y., Kemp, D. H. and Pearson, R. D. (1986). Int. J. Parasitol., 16, 27.
4. Laemmli, U. K. (1970). Nature 227. 680-685.
5. Montesino, R., Cremata, J., Rodríguez, M., Besada, V., Falcón, V. and de la Fuente, J. (1996). Biotechnol. Appl. Biochem. 23, 23-28.
6. Rand, K. N., Moore, T., Sriskantha, A., Spring, K., Tellam, R., Willadsen, P. and Cobon, G. (1989). Proc. Natl. Acad. Sci. 86, 9657-9661.
7. Rodriguez, M., Rubiera, R., Penichet, M. L., Montesino, R., Cremata, J., Falcón, V., Sánchez, G., Bringas, R., Cordovés, C., Valdés, M., Lleonart, R., Herrera, L. and de la Fuente, J. (1994). J. Biotechnol. 33, 135-146.
8. Shih, Y. C., Prausnitz, J. M. and Blanch, H. W. (1992). Biotechnol. & Bioengin. 40. 1155-1164.
9. Tellam, R. L., Smith, D. H., Kemp, D. H. and Willadsen, P. (1992). Vaccination against ticks. In: Yong, W. K. (Ed.), Animal Parasite Control Utilizing Biotechnology. CRC Press Inc. pp 302-331.
10. Turnbull, I. F., Smith, D. R. J., Sharp, P. J., Cobon, G. S. and Hynes, M. J. (1990). Appl. Envirom. Microbiol. 56, 2847-2852.