Cloning and characterization of the 5α-reductase type 2 promoter in the rat epididymis

Biology of Reproduction

Volumn 72, Issue 4, 2005, Pages 851-861

  Seenundun, Shayesta ^a   Robaire, Bernard ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

Epididymis; Gene regulation; Male reproductive tract; Steroid hormones; Testosterone

Indexed keywords

ANDROGEN; ANDROSTANOLONE; STEROID 5ALPHA REDUCTASE; STEROID 5ALPHA REDUCTASE 2; TESTOSTERONE; UNCLASSIFIED DRUG; DNA BINDING PROTEIN; MESSENGER RNA; SP3 PROTEIN, RAT; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR SP1; TRANSCRIPTION FACTOR SP3;

ADOLESCENT; ANIMAL CELL; ARTICLE; BASE PAIRING; CONTROLLED STUDY; EPIDIDYMIS; GEL MOBILITY SHIFT ASSAY; GENE MAPPING; GENETIC TRANSFECTION; IMMUNOLOCALIZATION; MALE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; RAT; START CODON; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION REGULATION; ANIMAL; CODON; GENE EXPRESSION REGULATION; GENE LIBRARY; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; WISTAR FURTH RAT;

CAPUT;

ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; CODON; DNA-BINDING PROTEINS; EPIDIDYMIS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE LIBRARY; MALE; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); RATS; RATS, INBRED WF; RNA, MESSENGER; SP1 TRANSCRIPTION FACTOR; SP3 TRANSCRIPTION FACTOR; TESTOSTERONE 5-ALPHA-REDUCTASE; TRANSCRIPTION FACTORS; TRANSCRIPTION INITIATION SITE;

EID: 15544367568     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod.104.033746     Document Type: Article

References (56)

1. Wilson JD. Metabolism of testicular androgens. Handbook Physiol 1975; 5:491-508.
2. Wilson JD. Sexual differentiation. Annu Rev Physiol 1978; 40:279-306.
3. Wilson JD. The pathogenesis of benign prostatic hyperplasia. Am J Med 1980; 68:745-756.
4. McConnell JD, Wilson JD, George FW, Geller J, Pappas F, Stoner E. Finasteride, an inhibitor of 5 alpha-reductase, suppresses prostatic dihydrotestosterone in men with benign prostatic hyperplasia. J Clin Endocrinol Metab 1992; 74:505-508.
5. Russell DW, Wilson JD, Steroid 5 alpha-reductase: two genes/two enzymes. Annu Rev Biochem 1994; 63:25-61.
6. Bingham KD, Shaw DA. The metabolism of testosterone by human male scalp skin. J Endocrinol 1973; 57:111-121.
7. Thiboutot D, Harris G, Iles V, Cimis G, Gilliland K, Hagari S. Activity of the type 1 5 alpha-reductase exhibits regional differences in isolated sebaceous glands and whole skin. J Invest Dermatol 1995; 105:209-214.
8. Takayasu S, Wakimoto H, Itami S, Sano S. Activity of testosterone 5 alpha-reductase in various tissues of human skin. J Invest Dermatol 1980; 74:187-191.
9. Serafini P, Lobo RA. Increased 5 alpha-reductase activity in idiopathic hirsutism. Fertil Steril 1985; 43:74-78.
10. Wilson JD, Gloyna RE. The intranuclear metabolism of testosterone in the accessory organs of reproduction. Recent Prog Horm Res 1970; 26:309-336.
11. Robaire B, Hermo L. Efferent ducts, epididymis, and vas deferens: structure, functions, and their regulation. In: Knobil E, Neill J. (eds.). The Physiology of Reproduction. New York: Ravel Press: 1988:999-1080.
12. Reid B, Cleland K. The structure and function of the epididymis. 1. The histology of the rat epididymis. Austr J Zool 1957; 5:223-246.
13. Robaire B, Viger RS. Regulation of epididymal epithelial-cell functions. Biol Reprod 1995; 52:226-236.
14. Berman DM, Russell DW. Cell-type-specific expression of rat steroid 5 alpha-reductase isozymes. Proc Natl Acad Sci USA 1993; 90: 9359-9363.
15. Silver RI, Wiley EL, Thigpen AE, Guileyardo JM, McConnell JD, Russell DW. Cell-type-specific expression of steroid 5-alpha-reductase 2. J Urol 1994; 152:438-442.
16. Andersson S, Bishop RW, Russell DW. Expression cloning and regulation of steroid 5 alpha-reductase, an enzyme essential for male sexual differentiation. J Biol Chem 1989; 264:16249-16255.
17. Andersson S, Berman DM, Jenkins EP, Russell DW. Deletion of steroid 5 alpha-reductase 2 gene in male pseudohermaphroditism. Nature 1991; 354:159-161.
18. Andersson S, Russell DW. Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases. Proc Natl Acad Sci U S A 1990; 87:3640-3644.
19. Normington K, Russell DW. Tissue distribution and kinetic characteristics of rat steroid 5 alpha-reductase isozymes. Evidence for distinct physiological functions. J Biol Chem 1992; 267:19548-19554.
20. Jenkins EP, Andersson S, Imperato-McGinley J, Wilson JD, Russell DW. Genetic and pharmacological evidence for more than one human steroid 5 alpha-reductase. J Clin Invest 1992; 89:293-300.
21. Thigpen AE, Davis DL, Milatovich A, Mendonca BB, Imperato-McGinley J, Griffin JE, Francke U, Wilson JD, Russell DW. Molecular genetics of steroid 5 alpha-reductase 2 deficiency. J Clin Invest 1992; 90:799-809.
22. Jenkins EP, Hsieh CL, Milatovich A, Normington K, Berman DM, Francke U, Russell DW. Characterization and chromosomal mapping of a human steroid 5 alpha-reductase gene and pseudogene and mapping of the mouse homologue. Genomics 1991; 11:1102-1112.
23. Labrie F, Sugimoto Y, Luu-The V, Simard S, Lachance Y, Bachvarov D, Leblanc G, Durocher F, Paquet N. Structure of human type II 5 alpha-reductase gene. Endocrinology 1992; 131:1571-1573.
24. Wigley WC, Prihoda JS, Mowszowicz I, Mendonca BB, New MI, Wilson JD, Russell DW. Natural mutagenesis study of the human steroid 5 alpha-reductase 2 isozyme. Biochemistry 1994; 33:1265-1270.
25. Thigpen AE, Silver RI, Guileyardo JM, Casey ML, McConnell JD, Russell DW. Tissue distribution and ontogeny of steroid 5 alpha-reductase isozyme expression. J Clin Invest 1993; 92:903-910.
26. Viger RS, Robaire B. The mRNAs for the steroid 5 alpha-reductase isozymes, types 1 and 2, are differentially regulated in the rat epididymis. J Androl 1996; 17:27-34.
27. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K. Current Protocols in Molecular Biology. New York: John Wiley and Sons; 1988.
28. Serre V, Robaire B. Distribution of immune cells in the epididymis of the aging Brown Norway rat is segment-specific and related to the luminal content. Biol Reprod 1999; 61:705-714.
29. Araki Y, Suzuki K, Matusik RJ, Obinata M, Orgebin-Crist MC. Immortalized epididymal cell lines from transgenic mice overexpressing temperature-sensitive simian vims 40 large T-antigen gene. J Androl 2002; 23:854-869.
30. Saksena SK, Lau IF, Chang MC. Age dependent changes in the sperm population and fertility in the male rat. Exp Aging Res 1979; 5:373-381.
31. Viger RS, Robaire B. Immunocytochemical localization of 4-ene steroid 5 alpha-reductase type 1 along the rat epididymis during postnatal development. Endocrinology 1994; 134:2298-2306.
32. Papp S, Robaire B, Hermo L. Immunocytochemical localization of the Ya, Yc, Yb1, and Yb2 subunits of glutathione S-transferases in the testis and epididymis of adult rats. Microsc Res Tech 1995; 30:1-23.
33. Douglass J, Garrett SH, Garrett JE. Differential patterns of regulated gene expression in the adult rat epididymis. Ann N Y Acad Sci 1991; 637:384-398.
34. Coleman L, Harris A. Immortalization of male genital duct epithelium: an assay system for the cystic fibrosis gene. J Cell Sci 1991; 98(pt 1):85-89.
35. Telgmann R, Brosens JJ, Kappler-Hanno K, Iveil R, Kirchhoff C. Epididymal epithelium immortalized by simian virus 40 large T antigen: a model to study epididymal gene expression. Mol Hum Reprod 2001; 7:935-945.
36. Matsui D, Sakari M, Sato T, Murayama A, Takada I, Kim M, Takeyama K, Kato S. Transcriptional regulation of the mouse steroid 5alpha-reductase type II gene by progesterone in brain. Nucleic Acids Res 2002; 30:1387-1393.
37. Lareyre JJ, Reid K, Nelson C, Kasper S, Rennie PS, Orgebin-Crist MC, Matusik RJ. Characterization of an androgen-specific response region within the 5′ flanking region of the murine epididymal retinoic acid binding protein gene. Biol Reprod 2000; 63:1881-1892.
38. Kaighn ME, Narayan KS, Ohnuki Y, Lechner JF, Jones LW. Establishment and characterization of a human prostatic carcinoma cell line (PC-3). Invest Urol 1979; 17:16-23.
39. Fry CJ, Farnham PJ. Context-dependent transcriptional regulation. J Biol Chem 1999; 274:29583-29586.
40. Philipsen S, Suske G. A tale of three fingers: the family of mammalian Sp/XKLF transcription factors. Nucleic Acids Res 1999; 27:2991-3000.
41. Bouwman P, Philipsen S. Regulation of the activity of Sp1-related transcription factors. Mol Cell Endocrinol 2002; 195:27-38.
42. Courey AJ, Holtzman DA, Jackson SP, Tjian R. Synergistic activation by the glutamine-rich domains of human transcription factor Sp1. Cell 1989; 59:827-836.
43. Liu Z, Simpson ER. Steroidogenic factor 1 (SF-1) and SP1 are required for regulation of bovine CYP11A gene expression in bovine luteal cells and adrenal Y1 cells. Mol Endocrinol 1997; 11:127-137.
44. Koutsodontis G, Tentes I, Papakosta P, Moustakas A, Kardassis D. Sp1 plays a critical role in the transcriptional activation of the human cyclin-dependent kinase inhibitor p21(WAF1/Cip1) gene by the p53 tumor suppressor protein. J Biol Chem 2001; 276:29116-29125.
45. Look DC, Pelletier MR, Tidwell RM, Roswit WT, Holtzman MJ. Stat1 depends on transcriptional synergy with Sp1. J Biol Chem 1995; 270: 30264-30267.
46. Merika M, Orkin SH. Functional synergy and physical interactions of the erythroid transcription factor GATA-1 with the Kruppel family proteins Sp1 and EKLF. Mol Cell Biol 1995; 15:2437-2447.
47. Noti JD, Reinemann BC, Petrus MN. Sp1 binds two sites in the CD11c promoter in vivo specifically in myeloid cells and cooperates with AP1 to activate transcription. Mol Cell Biol 1996; 16:2940-2950.
48. Perkins ND, Agranoff AB, Pascal E, Nabel GJ. An interaction between the DNA-binding domains of RelA(p65) and Sp1 mediates human immunodeficiency virus gene activation. Mol Cell Biol 1994; 14: 6570-6583.
49. Porter W, Wang F, Wang W, Duan R, Safe S. Role of estrogen receptor/Sp1 complexes in estrogen-induced heat shock protein 27 gene expression. Mol Endocrinol 1996; 10:1371-1378.
50. Lan ZJ, Lye RJ, Holic N, Labus JC, Hinton BT. Involvement of polyomavirus enhancer activator 3 in the regulation of expression of gamma-glutamyl transpeptidase messenger ribonucleic acid-IV in the rat epididymis. Biol Reprod 1999; 60:664-673.
51. Gill G, Pascal E, Tseng ZH, Tjian R. A glutamine-rich hydrophobic patch in transcription factor Sp1 contacts the dTAFII1 10 component of the Drosophila TFIID complex and mediates transcriptional activation. Proc Natl Acad Sci U S A 1994; 91:192-196.
52. Hagen G, Muller S, Beato M, Suske G. Sp1-mediated transcriptional activation is repressed by Sp3. EMBO J 1994; 13:3843-3851.
53. Lan ZJ, Palladino MA, Rudolph DB, Labus JC, Hinton BT. Identification, expression, and regulation of the transcriptional factor polyomavirus enhancer activator 3, and its putative role in regulating the expression of gamma-glutamyl transpeptidase mRNA-IV in the rat epididymis. Biol Reprod 1997; 57:186-193.
54. Vernet P, Faure J, Dufaure JP, Drevet JR. Tissue and developmental distribution, dependence upon testicular factors and attachment to spermatozoa of GPX5, a murine epididymis-specific glutathione peroxidase. Mol Reprod Dev 1997; 47:87-98.
55. Sivashanmugam P, Hall SH, Hamil KG, French FS, O'Rand MG, Richardson RT. Characterization of mouse Eppin and a gene cluster of similar protease inhibitors on mouse chromosome 2. Gene 2003; 312:125-134.
56. Cornwall GA, Orgebin-Crist MC, Hann SR. The CRES gene: a unique testis-regulated gene related to the cystatin family is highly restricted in its expression to the proximal region of the mouse epididymis. Mol Endocrinol 1992; 6:1653-1664.