In vitro reconstitution of an NADPH-dependent superoxide reduction pathway from Pyrococcus furiosus

Applied and Environmental Microbiology

Volumn 71, Issue 3, 2005, Pages 1522-1530

  Grunden, Amy M ^b   Jenney Jr , Francis E ^a   Ma, Kesen ^a,c   Ji, Mikyoung ^b   Weinberg, Michael V ^a   Adams, Michael W W ^a  

^a University of Georgia ^*  (United States)

^b NORTH CAROLINA STATE UNIVERSITY   (United States)

^c UNIVERSITY OF WATERLOO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; ELECTRONS; ENZYMES; HYDROGEN PEROXIDE; OXIDES; PROTEINS; REDUCTION; THERMAL EFFECTS;

FLAVIN ADENINE DINUCLEOTIDE (FAD); RECOMBINANT PROTEINS; RUBREDOXIN (RD); SUPEROXIDE REDUCTASE (SOR);

DETOXIFICATION;

FLAVINE ADENINE NUCLEOTIDE; FLAVINE MONONUCLEOTIDE; HYDROGEN PEROXIDE; OXIDOREDUCTASE; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE RUBREDOXIN OXIDOREDUCTASE; RUBREDOXIN; SUPEROXIDE; SUPEROXIDE REDUCTASE; UNCLASSIFIED DRUG;

REDUCTION;

ANAEROBIC BACTERIUM; ARTICLE; BACTERIAL GENE; DETOXIFICATION; ELECTRON; ENZYME RECONSTITUTION; ESCHERICHIA COLI; GENE SEQUENCE; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN MOTIF; PYROCOCCUS FURIOSUS; REDUCTION; SEQUENCE ANALYSIS; TEMPERATURE DEPENDENCE;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOPHYSICS; DNA, ARCHAEAL; GENES, ARCHAEAL; HYDROGEN PEROXIDE; KINETICS; MOLECULAR SEQUENCE DATA; NADH, NADPH OXIDOREDUCTASES; NADP; OXIDATION-REDUCTION; PYROCOCCUS FURIOSUS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUPEROXIDES;

ESCHERICHIA COLI; PYROCOCCUS FURIOSUS;

EID: 15444372151     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.3.1522-1530.2005     Document Type: Article

References (43)

1. Abreu, I. A., L. M. Saraiva, J. Carita, H. Huber, K. O. Stetter, D. Cabelli, and M. Teixeira. 2000. Oxygen detoxification in the strict anaerobic archaeon Archaeoglobus fulgidus: superoxide scavenging by neelaredoxin. Mol. Microbiol. 38:322-334.
2. Abreu, I. A., L. M. Saraiva, C. M. Soares, M. Teixeira, and D. E. Cabelli. 2001. The mechanism of superoxide scavenging by Archaeoglobus fulgidus neelaredoxin. J. Biol. Chem. 276:38995-39001.
3. Abreu, I. A., A. V. Xavier, J. LeGall, D. E. Cabelli, and M. Teixeira. 2002. Superoxide scavenging by neelaredoxin: dismutation and reduction activities in anaerobes. J. Biol. Inorg. Chem. 7:668-674.
4. Adams, M. W. W., F. E. Jenney, Jr., M. D. Clay, and M. K. Johnson. 2002. Superoxide reductase: fact or fiction? J. Biol. Inorg. Chem. 7:647-652.
5. Adams, M. W. W., and A. Kletzin. 1996. Oxidoreductase-type enzymes and redox proteins involved in fermentative metabolisms of hyperthermophilic Archaea. Adv. Protein Chem. 48:101-180.
6. Adams, M. W. W. 1999. The biochemical diversity of life near and above 100°C in marine environments. J. Appl. Microbiol. 85:108S-117S.
7. Ascenso, C., F. Rusnak, I. Cabrito, M. J. Lima, S. Naylor, I. Moura, and J. J. Moura. 2000. Desulfoferrodoxin: a modular protein. J. Biol. Inorg. Chem. 5:720-729.
8. Blake, P. R., J. B. Park, F. O. Bryant, S. Aono, J. K. Magnuson, E. Eccleston, J. B. Howard, M. F. Summers, and M. W. W. Adams. 1991. Determinants of protein hyperthermostability: purification and amino acid sequence of rubredoxin from the hyperthermophilic archaebacterium Pyrococcus furiosus and secondary structure of the zinc adduct by NMR. Biochemistry 30:10885-10895.
9. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
10. Bult, C. J., O. White, G. J. Olsen, L. Zhou, R. D. Fleischmann, G. G. Sutton, J. A. Blake, L. M. FitzGerald, R. A. Clayton, J. D. Gocayne, A. R. Kerlavage, B. A. Dougherty, J. F. Tomb, M. D. Adams, C. I. Reich, R. Overbeek, E. F. Kirkness, K. G. Weinstock, J. M. Merrick, A. Glodek, J. L. Scott, N. S. Geoghagen, and J. C. Venter. 1996. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science 273:1058-1073.
11. Clay, M. D., F. E. Jenney, Jr., P. L. Hagedoorn, G. N. George, M. W. W. Adams, and M. K. Johnson. 2002. Spectroscopic studies of Pyrococcus furiosus superoxide reductase: implications for active-site structures and the catalytic mechanism. J. Am. Chem. Soc. 124:788-805.
12. Day, M. W., B. T. Hsu, L. Joshua-Tor, J. B. Park, Z. H. Zhou, M. W. W. Adams, and D. C. Rees. 1992. X-ray crystal structures of the oxidized and reduced forms of the rubredoxin from the marine hyperthermophilic archaebacterium Pyrococcus furiosus. Protein Sci. 1:1494-1507.
13. Eggink, G., H. Engel, G. Vriend, P. Terpstra, and B. Witholt. 1990. Rubredoxin reductase of Pseudomonas oleovorans. Structural relationship to other flavoprotein oxidoreductases based on one NAD and two FAD fingerprints. J. Mol. Biol. 212:135-142.
14. Fiala, G., and K. O. Stetter. 1986. Pyrococcus-Furiosus Sp-Nov represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C. Arch. Microbiol. 145:56-61.
15. Fouquet, Y., A. Wafik, P. Cambon, C. Mevel, G. Meyer, and P. Gente. 1993. Tectonic setting and mineralogical and geochemical zonation in the snake pit sulfide deposit (mid-Atlantic ridge at 23-degrces-N). Econ. Geol. Bull. Soc. Econ. Geol. 88:2018-2036.
16. Gardner, A. M., R. A. Helmick, and P. R. Gardner. 2002. Flavorubredoxin, an inducible catalyst for nitric oxide reduction and detoxification in Escherichia coli. J. Biol. Chem. 277:8172-8177.
17. Gilles de Pelichy, L. D., and E. T. Smith. 1999. Redox properties of mesophilic and hyperthermophilic rubredoxins as a function of pressure and temperature. Biochemistry 38:7874-7880.
18. Guedon, E., and H. Petitdemange. 2001. Identification of the gene encoding NADH-rubredoxin oxidoreductase in Clostridium acetobutylicum. Biochem. Biophys. Res. Commun. 285:496-502.
19. Hagen, W. R. 1989. Direct electron transfer of redox proteins at the bare glassy carbon electrode. Eur. J. Biochem. 182:523-530.
20. Huber, R., P. Stoffers, J. L. Cheminee, H. H. Richnow, and K. O. Stetter. 1990. Hyperthermophilic archaebacteria within the crater and open-sea plume of erupting Macdonald seamount. Nature 345:179-182.
21. Jenney, F. E., Jr., and M. W. W. Adams. 2001. Rubredoxin from Pyrococcus furiosus. Methods Enzymol. 334:45-55.
22. Jenney, F. E., Jr., M. F. Vertagen, X. Cui, and M. W. W. Adams. 1999. Anaerobic microbes: oxygen detoxification without superoxide dismutase. Science 286:306-309.
23. Jovanovic, T., C. Ascenso, K. R. Hazlett, R. Sikkink, C. Krebs, R. Litwiller, L. M. Benson, I. Moura, J. J. Moura, J. D. Radolf, B. H. Huynh, S. Naylor, and F. Rusnak. 2000. Neelaredoxin, an iron-binding protein from the syphilis spirochete, Treponema pallidum, is a superoxide reductase. J. Biol. Chem. 275:28439-28448.
24. Kawarabayasi, Y., M. Sawada, H. Horikawa, Y. Haikawa, Y. Hino, S. Yamamoto, M. Sekine, S. Baba, H. Kosugi, A. Hosoyama, Y. Nagai, M. Sakai, K. Ogura, R. Otsuka, H. Nakazawa, M. Takamiya, Y. Ohfuku, T. Funuhashi, T. Tanaka, Y. Kudoh, J. Yamazaki, N. Kushida, A. Oguchi, K. Aoki, and H. Kikuchi. 1998. Complete sequence and gene organization of the gene organization of the genome of a hyperthermophilic archaebacterium, Pyrococcus horikoshii OT3. DNA Res. 5:147-155.
25. Klenk, H. P., R. A. Clayton, J. F. Tomb, O. White, K. E. Nelson, K. A. Ketchum, R. J. Dodson, M. Gwinn, E. K. Hickey, J. D. Peterson, D. L. Richardson, A. R. Kerlavage, D. E. Graham, N. C. Kyrpides, R. D. Fleischmann, J. Quackenbush, N. H. Lee, G. G. Sutton, S. Gill, E. F. Kirkness, B. A. Dougherty, K. McKenney, M. D. Adams, B. Loftus, J. C. Venter, et al. 1997. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus. Nature 390:364-370.
26. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
27. Lombard, M., M. Fontecave, D. Touati, and V. Niviere. 2000. Reaction of the desulfoferrodoxin from Desulfoarculus baarsii with superoxide anion. Evidence for a superoxide reductase activity. J. Biol. Chem. 275:115-121.
28. Lombard, M., C. Houee-Levin, D. Touati, M. Fontecave, and V. Niviere. 2001. Superoxide reductase from Desulfoarculus baarsii: reaction mechanism and role of glutamate 47 and lysine 48 in catalysis. Biochemistry 40:5032-5040.
29. Lombard, M., D. Touati, M. Fontecave, and V. Niviere. 2000. Superoxide reductase as a unique defense system against superoxide stress in the microaerophile Treponema pallidum. J. Biol. Chem. 275:27021-27026.
30. Lumppio, H. L., N. V. Shenvi, A. O. Summers, G. Voordouw, and D. M. Kurtz, Jr. 2001. Rubrerythrin and rubredoxin oxidoreductase in Desulfovibrio vulgaris: a novel oxidative stress protection system. J. Bacteriol. 183:101-108.
31. Ma, K., and M. W. W. Adams. 1999. A hyperactive NAD(P)H:rubredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus. J. Bacteriol. 181:5530-5533.
32. Nelson, K. E., R. A. Clayton, S. R. Gill, M. L. Gwinn, R. J. Dodson, D. H. Haft, E. K. Hickey, J. D. Peterson, W. C. Nelson, K. A. Ketchum, L. McDonald, T. R. Utterback, J. A. Malek, K. D. Linher, M. M. Garrett, A. M. Stewart, M. D. Cotton, M. S. Pratt, C. A. Phillips, D. Richardson, J. Heidelberg, G. G. Sutton, R. D. Fleischmann, J. A. Eisen, C. M. Fraser, et al. 1999. Evidence for lateral gene transfer between Archaea and bacteria from genome sequence of Thermotoga maritima. Nature 399:323-329.
33. Niviere, V., and M. Lombard. 2002. Superoxide reductase from Desulfoarculus baarsii. Methods Enzymol. 349:123-129.
34. Pick, E., and Y. Keisari. 1980. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J. Immunol. Methods 38:161-170.
35. Poole, L. B., and A. Claiborne. 1986. Interactions of pyridine nucleotides with redox forms of the flavin-containing NADH peroxidase from Streptococcus faecalis. J. Biol. Chem. 261:14525-14533.
36. Poole, L. B., and H. R. Ellis. 1996. Flavin-dependent alkyl hydroperoxide reductase from Salmonella typhimurium. 1. Purification and enzymatic activities of overexpressed AhpF and AhpC proteins. Biochemistry 35:56-64.
37. Robb, F. T., D. L. Maeder, J. R. Brown, J. DiRuggiero, M. D. Stump, R. K. Yeh, R. B. Weiss, and D. M. Dunn. 2001. Genomic sequence of hyperthermophile Pyrococcus furiosus: implications for physiology and enzymology. Methods Enzymol. 330:134-157.
38. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N. Y.
39. Silva, G., S. Oliveira, C. M. Gomes, I. Pacheco, M. Y. Liu, A. V. Xavier, M. Teixeira, J. Legall, and C. Rodrigues-Pousada. 1999. Desulfovibrio gigas neelaredoxin. A novel superoxide dismutase integrated in a putative oxygen sensory operon of an anaerobe. Eur. J. Biochem. 259:235-243.
40. Summit, M., and J. A. Baross. 1998. Thermophilic subseafloor microorganisms from the 1996 North Gorda Ridge eruption. Deep-Sea Res. Topical Studies Oceanogr. 45:2751-2766.
41. Tartaglia, L. A., G. Storz, M. H. Brodsky, A. Lai, and B. N. Ames. 1990. Alkyl hydroperoxide reductase from Salmonella typhimurium. Sequence and homology to thioredoxin reductase and other flavoprotein disulfide oxidoreductases. J. Biol. Chem. 265:10535-10540.
42. Weinberg, M. V., F. E. Jenney, Jr., X. Cui, and M. W. W. Adams. Rubrerythrin from the hyperthermophilic archaeon Pyrococcus furiosus is a rubredoxin-dependent, iron-containing peroxidase. J. Bacteriol. 186:7888-7895.
43. Yeh, A. P., Y. Hu, F. E. Jenney, Jr., M. W. W. Adams, and D. C. Rees. 2000. Structures of the superoxide reductase from Pyrococcus furiosus in the oxidized and reduced states. Biochemistry 39:2499-2508.