Construction of an efficient expression system for aspergillus isopullulanase in pichia pastoris, and a simple purification method

Bioscience, Biotechnology and Biochemistry

Volumn 67, Issue 5, 2003, Pages 1149-1153

  Akeboshi, Hiromi ^a   Kashiwagi, Yutaka ^b   Aoki, Hiroyoshi ^a,c   Tonozuka, Takashi ^a   Nishikawa, Atsushi ^a   Sakano, Yoshiyuki ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b NATIONAL FOOD RESEARCH INSTITUTE   (Japan)

^c Fuence Co Ltd   (Japan)

Author keywords

Aspergillus niger; Heterologous expression; Isopullulanase; Pichia pastoris

Indexed keywords

ASPERGILLUS; ASPERGILLUS NIGER; ASPERGILLUS ORYZAE; PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

ACID PHOSPHATASE; GLYCOSIDASE; ISOPULLULANASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIGER; ASPERGILLUS ORYZAE; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; PH; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; SACCHAROMYCES CEREVISIAE; THIN LAYER CHROMATOGRAPHY;

ACID PHOSPHATASE; AMINO ACID SEQUENCE; ASPERGILLUS NIGER; ASPERGILLUS ORYZAE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, THIN LAYER; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GLYCOSIDE HYDROLASES; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PICHIA; SACCHAROMYCES CEREVISIAE;

EID: 1542649848     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.67.1149     Document Type: Article

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