A non-B-DNA structure at the Bcl-2 major breakpoint region is cleaved by the RAG complex

Nature

Volumn 428, Issue 6978, 2004, Pages 88-93

  Raghavan, Sathees C ^a,b   Swanson, Patrick C ^c   Wu, Xlantuo ^a   Hsieh, Chih Lin ^a,b   Lieber, Michael R ^a,b  

^a UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; CHROMOSOMES; ENZYMES; GENETIC ENGINEERING; IMMUNOLOGY; TUMORS;

TRANSLOCATION;

DNA;

DNA B; DOUBLE STRANDED DNA; IMMUNOGLOBULIN HEAVY CHAIN; NUCLEIC ACID BINDING PROTEIN; PROTEIN BCL 2; RAG PROTEIN; UNCLASSIFIED DRUG; DNA; DNA BINDING PROTEIN; HOMEODOMAIN PROTEIN; HYDROGEN SULFITE; NUCLEAR PROTEIN; RAG 1 PROTEIN; RAG-1 PROTEIN; RAG2 PROTEIN, HUMAN; RAG2 PROTEIN, MOUSE; SULFITE; V(D)J RECOMBINATION ACTIVATING PROTEIN 2;

BIOLOGY;

ALLELE; ARTICLE; BASE PAIRING; CHROMOSOME 18; CHROMOSOME TRANSLOCATION; CHROMOSOME TRANSLOCATION 14; DNA CLEAVAGE; DNA RECOMBINATION; DNA STRAND BREAKAGE; DNA STRUCTURE; EPISOME; HUMAN; HUMAN CELL; HUMAN CHROMOSOME; IMMUNOGLOBULIN VARIABLE REGION; PRIORITY JOURNAL; PROTEIN DEGRADATION; SOMATIC CELL; ANIMAL; CELL LINE; CHEMISTRY; CHROMOSOME 14; CHROMOSOME BREAKAGE; CONFORMATION; GENE TRANSLOCATION; GENETIC RECOMBINATION; GENETICS; MACROMOLECULE; METABOLISM; MOUSE; PLASMID; PROTO ONCOGENE;

ANIMALS; CELL LINE; CHROMOSOME BREAKAGE; CHROMOSOMES, HUMAN, PAIR 14; CHROMOSOMES, HUMAN, PAIR 18; DNA; DNA-BINDING PROTEINS; GENES, BCL-2; HOMEODOMAIN PROTEINS; HUMANS; MACROMOLECULAR SUBSTANCES; MICE; NUCLEAR PROTEINS; NUCLEIC ACID CONFORMATION; PLASMIDS; PROTO-ONCOGENE PROTEINS C-BCL-2; RECOMBINATION, GENETIC; SULFITES; TRANSLOCATION, GENETIC;

EID: 1542287213     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/nature02355     Document Type: Article

References (18)

1. Lieber, M. R. in The Causes and Consequences of Chromosomal Aberrations (ed. Kirsch, I.) 239-275 (CRC Press, Boca Raton, 1993).
2. Lewis, S. M. The mechanism of V(D)J joining: lessons from molecular, immunological and comparative analyses. Adv. Immunol. 56, 27-150 (1994).
3. Dalla-Favera, R. in The Causes and Consequences of Chromosomal Aberrations (ed. Kirsch, I. R.) 313-332 (CRC Press, Boca Raton, 1993).
4. Korsmeyer, S. J. Chromosomal translocations in lymphoid malignancies reveal novel protooncogenes. Annu. Rev. Immunol. 10, 785-807 (1992).
5. Tycko, B. & Sklar, J. Chromosomal translocations in lymphoid neoplasia: A reappraisal of the recombinase model. Cancer Cells 2, 1-8 (1990).
6. Raghavan, S. C., Kirsch, I. R. & Lieber, M. R. Analysis of the V(D)J recombination efficiency at lymphoid chromosomal translocation breakpoints. J. Biol. Chem. 276, 29126-29133 (2001).
7. Jager, U. et al. Follicular lymphomas BCL-2/IgH junctions contain templated nucleotide insertions: novel insights into the mechanism of t(14;18) translocation. Blood 95, 3520-3529 (2000).
8. Gauss, G. H. & Lieber, M. R. Mechanistic constraints on diversity in human V(D)J recombination. Mol. Cell. Biol. 16, 258-269 (1996).
9. Schwarz, K. et al. RAG mutations in human B cell-negative SCID. Science 274, 97-99 (1996).
10. Swanson, P. C. The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination. Mol. Cell. Biol. 21, 449-458 (2001).
11. Yu, K., Chedin, F., Hsieh, C.-L., Wilson, T. E. & Lieber, M. R. R-loops at immunoglobulin class switch regions in the chromosomes of stimulated B cells. Nature Immunol. 4, 442-451 (2003).
12. Gough, G. W., Sullivan, K. M. & Lilley, D. M. The structure of cruciforms in supercoiled DNA: probing the single-stranded character of nucleotide bases with bisulphite. EMBO J. 5, 191-196 (1986).
13. Tevelev, A. & Schatz, D. G. Intermolecular V(D)J recombination. J. Biol. Chem. 275, 8341-8348 (2000).
14. Santagata, S. et al. The RAG1/RAG2 complex constitutes a 3′ flap endonuclease: implications for junctional diversity in V(D)J and transpositional recombination. Mol. Cell 4, 935-947 (1999).
15. Ramsden, D. A., McBlane, J. F., van Gent, D. C. & Gellert, M. Distinct DNA sequence and structure requirements for the two steps of V(D)J recombination signal cleavage. EMBO J. 15, 3197-3206 (1996).
16. Besmer, E. et al. Hairpin coding end opening is mediated by the recombination activating genes RAG1 and RAG2. Mol. Cell 2, 817-828 (1998).
17. Ma, Y., Pannicke, U., Schwarz, K. & Lieber, M. R. Hairpin opening and overhang processing by an Artemis:DNA-PKcs complex in V(D)J recombination and in nonhomologous end joining. Cell 108, 781-794 (2002).
18. Yu, K. & Lieber, M. R. The nicking step of V(D)J recombination is independent of synapsis: implications for the immune repertoire. Mol. Cell. Biol. 20, 7914-7921 (2000).