Substrate binding to fluorescent labeled wild type, Lys213Arg, and His233Gln Saccharomyces cerevisiae phosphoenolpyruvate carboxykinases

International Journal of Biochemistry and Cell Biology

Volumn 36, Issue 5, 2004, Pages 861-869

  Bueno, Claudia ^a   González Nilo, Fernando D ^a   Victoria Encinas, María ^a   Cardemil, Emilio ^a  

^a UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

Author keywords

HEPES; High pressure liquid chromatography; HPLC; Lamarckian genetic algorithm; LGA; N (2 hydroxyethyl)piperazine N 2(ethanesulfonic acid); P pyridoxyl; PEP; Phosphoenolpyruvate; Phosphopyridoxyl; PLP; Pyridoxal 5 phosphate; TFA

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE DIPHOSPHATE MANGANESE; ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE MANGANESE; ARGININE; FLUORESCENT DYE; GLUTAMINE; HISTIDINE; LIGAND; LYSINE; MANGANESE; MANGANESE DERIVATIVE; MUTANT PROTEIN; NITROGEN; OXYGEN; PHOSPHOENOLPYRUVATE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); PYRIDOXINE DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CATALYSIS; CONTROLLED STUDY; DERIVATIZATION; DISSOCIATION; ENZYME ACTIVITY; FLUORESCENCE; NONHUMAN; PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; SYNTHESIS; THERMODYNAMICS; WILD TYPE;

AMINO ACID SUBSTITUTION; MUTATION; PHOSPHOENOLPYRUVATE; PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP); PROTEIN BINDING; SACCHAROMYCES CEREVISIAE; SPECTROMETRY, FLUORESCENCE; STAINING AND LABELING; STRUCTURAL HOMOLOGY, PROTEIN; SUBSTRATE SPECIFICITY;

LAMARCKIA; SACCHAROMYCES CEREVISIAE;

EID: 1542285126     PISSN: 13572725     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biocel.2003.09.008     Document Type: Article

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