Ligand interactions and protein conformational changes of phosphopyridoxyl-labeled Escherichia coli phosphoenolpyruvate carboxykinase determined by fluorescence spectroscopy

European Journal of Biochemistry

Volumn 269, Issue 20, 2002, Pages 4960-4968

  Encinas, María Victoria ^a   González Nilo, Fernando D ^a   Goldie, Hughes ^b   Cardemil, Emilio ^a  

^a UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

^b UNIVERSITY OF SASKATCHEWAN   (Canada)

Author keywords

Conformational changes; Escherichia coli phosphoenolpyruvate carboxy kinase; Ligand binding; P pyridoxyl fluorescence spectroscopy

Indexed keywords

ACRYLAMIDE; LIGAND; MAGNESIUM ION; MANGANESE; METAL COMPLEX; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP); SOLVENT;

ARTICLE; BINDING AFFINITY; DISSOCIATION CONSTANT; ENZYME BINDING; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; MOLECULAR MODEL; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; CARBON DIOXIDE; ESCHERICHIA COLI PROTEINS; LIGANDS; LYSINE; MAGNESIUM; MANGANESE; MODELS, MOLECULAR; PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP); PROTEIN CONFORMATION; PYRIDOXINE; SCHIFF BASES; SERINE; SPECTROMETRY, FLUORESCENCE;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0036411895     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2002.03196.x     Document Type: Article

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