Evaluation of cytotoxic function and apoptosis in interleukin (IL)-12/IL-15-treated umbilical cord or adult peripheral blood natural killer cells by a propidium-iodide based flow cytometry

Pediatric Allergy and Immunology

Volumn 15, Issue 1, 2004, Pages 79-85

  Lin, Syh Jae ^a   Cheng, Po Jen ^a   Huang, Yen Ju ^a   Kuo, Ming Ling ^a,b  

^a CHANG GUNG UNIVERSITY   (Taiwan)

^b CHANG GUNG CHILDREN S HOSPITAL   (Taiwan)

Author keywords

Apoptosis; Cord blood; Cytokines; Cytometry; Natural killer cells

Indexed keywords

INTERLEUKIN 12; INTERLEUKIN 15; PROPIDIUM IODIDE;

ADULT; APOPTOSIS; ARTICLE; CELL FUNCTION; CELL STRAIN K 562; CONTROLLED STUDY; CYTOTOXICITY; DOSE RESPONSE; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOREGULATION; MALE; NATURAL KILLER CELL; NEWBORN; NORMAL HUMAN; PARAMETER; PRIORITY JOURNAL; UMBILICAL CORD BLOOD;

ADJUVANTS, IMMUNOLOGIC; ADULT; APOPTOSIS; BLOOD; CYTOTOXICITY, IMMUNOLOGIC; FEMALE; FETAL BLOOD; FLOW CYTOMETRY; HUMANS; INDICATORS AND REAGENTS; INFANT, NEWBORN; INTERLEUKIN-12; INTERLEUKIN-15; INTERLEUKINS; K562 CELLS; KILLER CELLS, NATURAL; MALE; PROPIDIUM;

EID: 1542268272     PISSN: 09056157     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0905-6157.2003.00103.x     Document Type: Article

References (34)

1. ROBERTSON MJ, RITZ J. Biology and clinical relevance of human natural killer cells. Blood 1990: 76: 2421-38.
2. BRADSTOCK KF, LUXFORD C, GRIMSLEY PG. Functional and phenotypic assessment of neonatal human leucocytes expressing natural killer cell-associated antigens. Immunol Cell Biol 1993: 71: 535-42.
3. GLUCKMAN E. Current status of umbilical cord blood hematopoietic stem cell transplantation. Exp Hematol 2000: 28: 1197-205.
4. HARRIS DT. In vitro and in vivo assessment of the graft-versus-leukemia activity of cord blood. Bone Marrow Transpl 1995: 15: 17-23.
5. KOHL S, Loo LS, GONICK B. Analysis in human neonates of defective antibody-dependent cellular cytotoxicity and natural killer cytotoxicity to herpes simplex virus-infected cells. J Inf Dis 1984: 150: 14-19.
6. YABUHARA A, KAWAI H, KOMIYAMA A. Development of natural killer cytotoxicity during childhood: marked increases in number of natural killer cells with adequate cytotoxic abilities during infancy to early childhood. Pediatr Res 1990: 28: 316-22.
7. NAIR MPN, SCHWARTZ SA, MENON M. Association of decreased natural and antibody-dependent cellular cytotoxicity and production of natural killer cytotoxic factor and interferon in neonates. Cell Immunol 1985: 94: 159-71.
8. NGUYEN QH, ROBERTS RL, ANK BJ, LIN SJ, LAU CK, STIEHM ER. Enhancement of antibody-dependent cellular cytotoxicity (ADCC) of neonatal cells by interleukin-2 (IL-2) and IL-12. Clin Diagn Lab Immunol 1998: 5: 98-104.
9. KOHL S. Human neonatal natural killer cell cytotoxicity function. Pediatr Inf Dis J 1999: 18: 635-7.
10. QIAN JX, LEE SM, SUEN Y, KNOPPEL E, VAN VEN DE C, CAIRO MS. Decreased interleukin-15 from activated cord versus adult peripheral blood mononuclear cells and the effect of interleukin-15 in upregulating antitumor immune activity and cytokine production in cord blood. Blood 1997: 90: 3106-17.
11. ROSS ME, CALIGIURI MA. Cytokine-induced apoptosis of human natural killer cells identifies a novel mechanism to regulate the innate immune response. Blood 1997: 89: 910-8.
12. JEWETT A, BONAVIDA B. Target-induced inactivation and cell death by apoptosis in a subset of human NK cells. J Immunol 1996: 156: 907-15.
13. TAGA K, YAMAUCHI A, KABASHIMA K, BLOOM ET, MULLER J, TOSATO G. Target-induced death by apoptosis in human lymphokine-activated natural killer cells. Blood 1996: 87: 2411-8.
14. GODOY-RAMIREZ K, FRANCK K, GAINES H. A novel method for the simultaneous assessment of natural killer cell conjugate formation and cytotoxicity at the single-cell level by multi-parameter flow cytometry. J Immunol Methods 2000: 239: 35-44.
15. LIN SJ, ROBERTS RL, ANK BJ, NGUYEN QH, THOMAS EK, STIEHM ER. Effect of interleukin (IL)-12 and IL-15 on activated natural killer (ANK) and antibody-dependent cellular activity (ADCC) in HIV infection. J Clin Immunol 1998: 18: 335-45.
16. LAU AS, SIGAROUNDINIA M, YOUNG MC, KOHL S. Interleukin-12 induces interferon gamma and natural killer cytotoxicity in cord blood mononuclear cells. Pediatr Res 1996: 39: 150-5.
17. SUEN Y, LEE SM, QIAN J, VAN VEN DE C, CAIRO MS. Dysregulation of lymphokine production in the neonate and its impact on neonatal cell mediated immunity. Vaccine 1998; 16: 1369-77.
18. LIN SJ, WANG LY, HUANG YJ, KUO ML. Proliferation and apoptosis induced by interleukin (IL)-12 and IL-15-umbilical cord versus adult peripheral blood mononuclear cells. Bone Marrow Transpl 2001: 28: 439-45.
19. SOARES MVD, BORTHWICK NJ, MAINI MK, JANOSSY G, SALMON M, AKBAR AN. IL-7-dependent extrathymic expansion of CD45RA + T cells enable preservation of a na repertoire. J Immunol 1998: 161: 5909-17.
20. DRENOU B, CHOQUEUX C, GHALBZOURI AE, et al. Characterization of the roles of CD95 and CD95 ligand in cord blood. Bone Marrow Transpl 1998: 22: S44-7.
21. NAORA H, GOUGOEN ML. Enhanced survival and potent expansion of the natural killer cell population of HIV-infected individuals by exogenous IL-15. Immunol Lett 1999: 68: 359-67.
22. CARSON WE, FEHNIGER TA, HALDAR S, et al. A potential role for interleukin-15 in the regulation of human natural killer cell survival. J Clin Invest 1997: 99: 937-43.
23. LiN SJ, YANG MH, CHAO HC, KUO ML, HUANG JL. Effect of IL-15 and Flt3-L ligand on natural killer cell expansion and activation-umbilical cord versus adult peripheral blood mononuclear cells. Pediatr Allergy Immunol 2000: 11: 168-74.
24. ARMANT M, DELESPESSE G, SARFATI M. IL-2 and IL-7 but not IL-12 protects natural killer cells from death by apoptosis and upregulate bcl-2 expression. Immunology 1995: 85: 331-7.
25. GARDINER CM, MEARA AO, REEN DJ. Differential cytotoxicity of cord blood and bone marrow-derived natural killer cells. Blood 1998: 91: 207-13.
26. KEEVER CA, ABU-HAJIR M, GRAF W, et al. Characterization of the alloreactivity and anti-leukemia reactivity of cord blood mononuclear cells. Bone Marrow Transpl 1995: 15: 407-19.
27. ZAMAI L, MARIANI AR, ZAULI G, et al. Kinetics of in vitro natural killer activity against K562 cells as detected by flow cytometry. Cytometry 1998: 32: 280-5.
28. KOHL S, SIGOUROUDINIA M, ENGLEMAN EG. Adhesion defect of antibody-mediated target cell binding of neonatal natural killer cells. Pediatr Res 1999: 46: 755-9.
29. YAMAUCHI A, TAGA K, MOSTOWSKI HS, BLOOM ET. Target cell-induced apoptosis of interleukin-2-activated human natural killer cells: role of surface molecules and intracellular events. Blood 1996: 87: 5127-35.
30. ORTALDO JR, MASON AT, O'SHEA JJ. Receptor-induced death in human natural killer cells: involvement of CD16. J Exp Med 1995: 181: 339-44.
31. HAUX J, JOHNSON AC, STEINKJER B, EGEBERG K, SUNDAN A, ESPEVIK T. The role of interleukin-2 in regulating the sensitivity of natural killer cells for Fas-mediated apoptosis. Cancer Immunol Immunother 1999: 48: 139-46.
32. LIN SJ, CHAO HC, Kuo ML. Effect of interleukin (IL)-15 and IL-12 on CD69 expression of T-lymphocytes and natural killer cells from umbilical cord blood. Biol Neonate 2000: 78: 181-5.
33. LIN SJ, YAN DC. ICAM-1(CD54) expression on T-lymphocytes and natural killer cells from umbilical cord blood regulation with interleukin (IL)-12 and IL-15. Cytokines, Cell Mol Ther 2000: 6: 161-4.
34. BRAHMI Z, HOMMEL-BERREY G, SMITH F, GHOMSON B. NK cells recover early and mediate cytotoxicity via perforin/granzyme and Fas/FasL pathways in umbilical cord blood recipients. Hum Immunol 2001: 62: 782-90.