Semiautomatic detection of DNA methylation at CpG islands

BioTechniques

Volumn 38, Issue 3, 2005, Pages 354-358

  Hong, Kyeong Man ^a   Yang, Sei Hoon ^a   Guo, Mingzhou ^b   Herman, James G ^b   Jen, Jin ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; AUTOMATION; CPG ISLAND; DNA METHYLATION; DNA MODIFICATION; DNA SEQUENCE; GENE AMPLIFICATION; HUMAN; NONHUMAN; POLYMERASE CHAIN REACTION; PROMOTER REGION;

EID: 15244348376     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/05383BM02     Document Type: Article

References (6)

1. Baylin, S.B. and J.G. Herman. 2000. DNA hypermethylation in tumorigenesis: epigenetics joins genetics. Trends Genet. 76:168-174.
2. Herman, J.G., J.R. Graff, S. Myohanen, B.D. Nelkin, and S.B. Baylin. 1996. Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc. Natl. Acad. Sci. USA 95:9821-9826.
3. Warnecke, P.M., C. Stirzaker, J.R. Melki, D.S. Millar, C.L. Paul, and S.J. Clark. 1997. Detection and measurement of PCR bias in quantitative methylation analysis of bisulphite-treated DNA. Nucleic Acids Res. 25:4422-4426.
4. Gonzalgo, M.L. and P.A. Jones. 1997. Rapid quantitation of methylation differences at specific sites using methylation-sensitive single nucleotide primer extension. (Ms-SNuPE). Nucleic Acids Res. 25:2529-2531.
5. Gonzalgo, M.L. and P.A. Jones. 2002. Quantitative methylation analysis using methylation-sensitive single-nucleotide primer extension (Ms-SNuPE). Methods 27:128-133.
6. El-Maarri, O., U. Herbiniaux, J. Walter, and J. Oldenburg. 2002. A rapid, quantitative, non-radioactive bisulfite-SNuPE-IP RP HPLC assay for methylation analysis at specific CpG sites. Nucleic Acids Res. 30:e25.