Ca2+ channel blockers verapamil and nifedipine inhibit apoptosis induced by 25-hydroxycholesterol in human aortic smooth muscle cells

Journal of Lipid Research

Volumn 38, Issue 10, 1997, Pages 2049-2061

  Ares, Mikko P S ^a   Pörn Ares, M Isabella ^a   Thyberg, Johan ^a   Juntti Berggren, Lisa ^a   Berggren, Per Olof ^a   Diczfalusy, Ulf ^b   Kallin, Bengt ^a   Björkhem, Ingemar ^b   Orrenius, Sten ^a   Nilsson, Jan ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Ca2+; Caspase 3; Chromatin condensation; CPP32; DNA fragmentation; Oxysterol

Indexed keywords

25 HYDROXYCHOLESTEROL; CALCIUM CHANNEL BLOCKING AGENT; CALCIUM ION; CASPASE 3; DNA FRAGMENT; GAMMA INTERFERON; NIFEDIPINE; PROTEINASE; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG; VERAPAMIL; 25-HYDROXYCHOLESTEROL; CALCIUM; CASP3 PROTEIN, HUMAN; CASPASE; CHOLESTEROL DERIVATIVE; CYSTEINE PROTEINASE; FLUORESCENT DYE; FURA 2;

AORTA MEDIA; APOPTOSIS; ARTICLE; CALCIUM CELL LEVEL; CELL PROTECTION; CHROMATIN CONDENSATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; AORTA; CELL CULTURE; CHROMATIN; CYTOCHEMISTRY; CYTOLOGY; DRUG EFFECT; ELECTRON MICROSCOPY; ENZYME ACTIVATION; METABOLISM; ULTRASTRUCTURE; VASCULAR SMOOTH MUSCLE; WESTERN BLOTTING;

AORTA; APOPTOSIS; BLOTTING, WESTERN; CALCIUM; CALCIUM CHANNEL BLOCKERS; CASPASE 3; CASPASES; CELLS, CULTURED; CHROMATIN; CYSTEINE ENDOPEPTIDASES; DNA FRAGMENTATION; ENZYME ACTIVATION; FLUORESCENT DYES; FURA-2; HISTOCYTOCHEMISTRY; HUMANS; HYDROXYCHOLESTEROLS; INTERFERON TYPE II; MICROSCOPY, ELECTRON; MUSCLE, SMOOTH, VASCULAR; NIFEDIPINE; TUMOR NECROSIS FACTOR-ALPHA; VERAPAMIL;

EID: 15144341601     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (57)

1. Hwang, P. L. 1992. Inhibitors of protein and RNA synthesis block the cytotoxic effects of oxygenated sterols. Biochim. Biophys. Acta. 1136: 5-11.
2. Imai, H., N. T. Werthessen, V. Subramanyam, P. W. LeQuesne, A. H. Soloway, and M. Kanisawa. 1980. Angiotoxicity of oxygenated sterols and possible precursors. Science. 207: 651-654.
3. Naseem, S. M., and F. P. Heald. 1987. Cytotoxicity of cholesterol oxides and their effects on cholesterol metabolism in cultured human aortic smooth muscle cells. Biochem. Int. 14: 71-84.
4. Brown, M. S., S. E. Dana, and J. L. Goldstein. 1975. Cholesterol ester formation in cultured human fibroblasts. Stimulation by oxygenated sterols. J. Biol. Chem. 250: 4025-4027.
5. Field, F. J., and S. N. Mathur. 1983. Regulation of acyl CoA: cholesterol acyltransferase by 25-hydroxycholesterol in rabbit intestinal microsomes and absorptive cells. J. Lipid Res. 24: 1049-1059.
6. Morin, R. J., and S-K. Peng. 1989. Effects of cholesterol oxidation derivatives on cholesterol esterifying and cholesteryl ester hydrolytic enzyme activity of cultured rabbit aortic smooth muscle cells. Lipids. 24: 217-220.
7. Mattson Hultén, L., H. Lindmark, U. Diczfalusy, I. Björkhein, M. Ottosson, Y. Liu, G. Bondjers, and O. Wiklund. 1996. Oxysterols present in atherosclerotic tissue decrease the expression of lipoprotein lipase messenger RNA in human monocyte-derived macrophages. J. Clin. Invest. 97: 461-468.
8. Aupeix, K., F. Toti, N. Satta, P. Bischoff, and J.-M. Freyssinet. 1996. Oxysterols induce membrane procoagulant activity in monocytic THP-1 cells. Biochem. J. 314: 1027-1033.
9. Kilsdonk, E. P. C., D. W. Morel, W. J. Johnson, and G. H. Rothblat. 1995. Inhibition of cellular cholesterol efflux by 25-hydroxycholesterol. J. Lipid Res. 36: 505-516.
10. 45Ca uptake: correlation with depressed DNA synthesis. J. Cell Physiol. 120: 151-156.
11. Guo, X., Y. Ohno, A. Miyajima, M. Sunouchi, and A. Takanaka. 1993. Oxysterols inhibit gap junctional communication between rat hepatocytes in primary culture. Pharmacol. Toxicol. 73: 10-13.
12. Johnson, K. A., C. J. Morrow, G. D. Knight, and T. J. Scallen. 1994. In vivo formation of 25-hydroxycholesterol from endogenous cholesterol after a single meal, dietary cholesterol challenge. J. Lipid Res. 35: 2241-2253.
13. Lin, C-Y., and D. W. Morel. 1996. Esterification of oxysterols in human serum: effects on distribution and cellular uptake. J. Lipid Res. 37: 168-178.
14. Saucier, S. E., A. A. Kandutsch, F. R. Taylor, T. A. Spencer, S. Phirwa, and A. K. Gayen. 1985. Identification of regulatory oxysterols, 24(S), 25-epoxycholesterol and 25-hydroxycholesterol, in cultured fibroblasts. J. Biol. Chem. 260: 14571-14579.
15. Björkhem, I., O. Andersson, U. Dicvfalusy, B. Sevastik, R. J. Xiu, C. Duan, and E. Lund. 1994. Atherosclerosis and sterol 27-hydroxylase: evidence for a role of this enzyme in elimination of cholesterol from human macrophages. Proc. Natl. Acad. Sci. USA. 91: 8592-8596.
16. Kerr, J. F. R., C. M. Winterford, and B. V. Harmon. 1994. Morphological criteria for identifying apoptosis. In Cell Biology. A Laboratory Handbook. J. E. Celis, editor. Academic Press, San Diego, CA. 319-329.
17. Majno, G., and I. Joris. 1995. Apoptosis, oncosis, and necrosis. An overview of cell death. Am. J. Pathol. 146: 3-15.
18. Fernandes-Alnemri, T., G. Litwack, and E. S. Alnemri. 1994. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1β-converting enzyme. J. Biol. Chem. 269: 30761-30764.
19. Nicholson, D. W. 1996. ICE/CED3-like proteases as therapeutic targets for the control of inappropriate apoptosis. Nat. Biotechnol. 14: 297-301.
20. Takeda, Y., H. Watanabe, S. Yonehara, T. Yamashita, S. Saito, and F. Sendo. 1993. Rapid acceleration of neutrophil apoptosis by tumor necrosis factor-alpha. Int. Immunol. 5: 691-694.
21. Munn, D. H., A. C. Beall, D. Song, R. W. Wrenn, and D. C. Throckmorton. 1995. Activation-induced apoptosis in human macrophages: developmental regulation of a novel cell death pathway by macrophage colony-stimulating factor and interferon gamma. J. Exp. Med. 181: 127-136.
22. Geng, Y. J., and P. Libby. 1995. Evidence for apoptosis in advanced human atheroma. Colocalization with interleukin-1β-converting enzyme. Am. J. Pathol. 147: 251-266.
23. Han, D. K., C. C. Handenschild, M. K. Hong, B. T. Tinkle, M. B. Leon, and G. Liau. 1995. Evidence fof apoptosis in human atherogenesis and in a rat vascular injury model. Am. J. Pathol. 147: 267-277.
24. Bennett, M. R., G. I. Evan, and S. M. Schwartz. 1995. Apoptosis of human vascular smooth muscle cells derived from normal vessels and coronary atherosclerotic plaques. J. Clin. Invest. 95: 2266-2274.
25. Christ, M., B. Luu, J. E. Mejia, I. Moosbrugger, and P. Bischoff. 1993. Apoptosis induced by oxysterols in murine lymphoma cells and in normal thymocytes. Immunology. 78: 455-460.
26. Aupeix, K., D. Weltin, J. E. Mejia, M. Christ, J. Marchal, J. M. Freyssinet, and P. Bischoff. 1995. Oxysterol-induced apoptosis in human monocytic cell lines. Immunobiology. 194: 415-428.
27. Collins, R. J., B. V. Harmon, G. C. Gobé, and J. F. R. Kerr. 1992. Internucleosomal DNA cleavage should not be the sole criterion foi identifying apoptosis. Int. J. Radiat. Biol. 61: 451-453.
28. 2+ in the pancreatic B-cell. FEBS Lett. 303: 85-90.
29. Gold, R., M. Schmied, G. Giegerich, H. Breitshopf, H. P. Hartung, K. V. Toyka, and H. Lassmann. 1994. Differentiation between cellular apoptosis and necrosis by the combined use of in situ tailing and nick translation techniques. Lab. Invest. 71: 219-225.
30. Rus, H. G., F. Niculescu, and R. Vlaicu. 1991. Tumor necrosis factor-alpha in human arterial wall with atherosclerosis. Atherosclerosis. 89: 247-254.
31. Szekanecz, Z., M. R. Shah, VV. H. Pearce, and A. E. Koch. 1994. Human atherosclerotic abdominal aortic aneurysms produce interleukin (IL)-6 and interferon-gamma but not IL-2 and IL-4: the possible role for IL-6 and interferon-gamma in vascular inflammation. Agents Actions 42: 159-162.
32. Chamley-Campbell, J., G. R. Campbell, and R. Ross. 1979. The smooth muscle cell in culture. Physiol. Rev. 59: 1-61.
33. 2+-ATPase inhibitor thapsigargin. Exp. Cell Res. 212: 84-92.
34. 2+ channels exposed to serum from patients with type I diabetes. Science. 261: 86-90.
35. Martikainen, P., and J. Isaacs. 1990. Role of calcium in the programmed death of rat prostatic glandular cells. Prostate. 17: 175-187.
36. Nicholson, D. W., A. Ali, N. A. Thornberry, J. P. Vaillancourt, C. K. Ding, M. Gallant, Y. Gareau, P. R. Griffin, M. Labelle, Y. A. Lazebnik, N. A. Munday, S. M. Raju, M. E. Smulson, T-T. Yamin, V. L. Yu, and D. K. Miller. 1995. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature. 376: 37-43.
37. Huang, Y. H., J. Rönnelid, and J. Frostegård. 1995. Oxidized LDL induces enhanced antibody formation and MHC class II-dependent IFN-γ production in lymphocytes from healthy individuals. Arterioscler. Thromb. Vasc. Biol. 15: 1577-1583.
38. Jovinge, S., M. P. S. Ares, B. Kallin, and J. Nilsson. 1996. Human monocytes/macrophages release TNF-α in response to ox-LDL. Arterioscler. Thrornb. Vasc. Biol. 16: 1573-1579.
39. Vaddi, K., F. A. Nicolini, P. Mehta, and J. L. Mehta. 1994. Increased secretion of tumor necrosis factor-alpha and interferon-gamma by mononuclear leukocytes in patients with ischemic heart disease. Relevance in superoxide anion generation. Circulation. 90: 694-699.
40. Tipping, P. G., and W. W. Hancock. 1993. Production of tumor necrosis factor and interleukin-1 by macrophages from human atheromatous plaques. Am. J. Pathol. 142: 1721-1727.
41. Jayaraman, T., and A. R. Marks. 1997. T cells deficient in inositol 1,4,5-trisphosphate receptor are resistant to apoptosis. Mol. Cell. Biol. 17: 3005-3012.
42. Orrenius, S., M. Ankarcrona, and P. Nicotera. 1996. Mechanisms of calcium-related cell death. Adv. Neurol. 71: 137-151.
43. Kandutsch, A. A., H. J. Heiniger, and H. W. Chen. 1977. Effects of 25-hydroxycholesterol and 7-ketocholesterol, inhibitors of sterol synthesis, administered orally to mice. Biochim. Biophys. Acta. 486: 260-272.
44. Aikawa, K., T. Furuchi, Y. Fujimoto, H. Arai, and K. Inoue. 1994. Structure-specific inhibition of lysosomal cholesterol transport in macrophages by varions steroids. Biochim. Biophys. Acta. 1213: 127-134.
45. Maor, I., and M. Aviram. 1994. Oxidized low density lipoprotein leads to macrophage accumulation of unesterified cholesterol as a result of lysosomal trapping of the lipoprotein hydrolyzed cholesteryl ester. J. Lipid Res. 35: 803-819.
46. Falk, E. 1992. Why do plaques rupture? Circulation. 86: (suppl III): 30-42.
47. Crisby, M., B. Kallin, J. Thyberg, B. Zhivotovsky, S. Orrenius, V. Kostulas, and J. Nilsson. 1997. Cell death in human atherosclerotic plaques involves both oncosis and apoptosis. Atherosclerosis. 130: 17-27.
48. Etingin, O. R., and D. P. Hajjar. 1990. Calcium channel blockers enhance cholesteryl ester hydrolysis and decrease total cholesterol accumulation in human aortic tissue. Circ. Res. 66: 185-190.
49. Jukema, J. W., A. H. Zwinderman, A. J. van Boven, J. H. Reiber, A. van der Laarse, K. I. Lie, and A. V. Bruschke. 1996. Evidence for a synergistic effect of calcium channel blockers with lipid-lowering therapy in retarding progression of coronary atherosclerosis in symptomatic patients with normal to moderately raised cholesterol levels. Arterioscler. Thromb. Vasc. Biol. 16: 425-430.
50. Waters, D., and J. Lesperance. 1994. Calcium channel blockers and coronary atherosclerosis: from the rabbit to the real world. Am. Heart J. 128: 1309-1316.
51. Furberg, C. D., B. M. Psaty, and J. V. Meyer. 1995. Nifedipine. Dose-related increase in mortality in patients with coronary heart disease. Circulation. 92: 1326-1331.
52. Gobel, E. J. A. M., R. W. M. Hautvast, W. H. van Gilst, J. N. Spanjaard, H. L. Hillege, M. J. L. Dejongste, G. P. Molhoek, and K. I. Lie. 1995. Randomised, doubled-blind trial of intravenous diltiazem versus glyceryl trinitrate for unstable angina pectoris. Lancet. 346: 1653-1657.
53. Reid, V. C., M. J. Mitchinson, and J. N. Skepper. 1993. Cytotoxicity of oxidized low-density lipoprotein to mouse peritoneal macrophages: an ultrastructural study. J. Pathol. 171: 321-328.
54. Reid, V. C., S. J. Hardwick, and M. J. Mitchinson. 1993. Fragmentation of DNA in P388D1 macrophages exposed to oxidized low-density lipoprotein. FEBS Lett. 332: 218-220.
55. Nishio, E., S. Arimura, and Y. Watanabe. 1996. Oxidized LDL induces apoptosis in cultured smooth muscle cells: a possible role for 7-ketocholesterol. Biochem. Biophys. Res. Commun. 223: 413-418.
56. Björkerud, B., and S. Björkerud. 1996. Contrary effects of lightly and strongly oxidized LDL with potent promotion of growth versus apoptosis on arterial smooth muscle cells, macrophages, and fibroblasts. Arterioscler. Thromb. Vasc. Biol. 166: 416-424.
57. Chisolm, G. M., G. Ma, K. C. Irwin, L. L. Martin, K. G. Gunderson, L. F. Linberg, D. W. Morel, and P. E. DiCorleto. 1994. 7β-Hydroperoxycholest-5-en-3β-ol, a component of human atherosclerotic lesions, is the primary cytotoxin of oxidized human low density lipoprotein. Proc. Natl. Acad. Sci. USA. 91: 11452-11456.