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Volumn , Issue 8, 2005, Pages 1016-1018

Mycobacterium tuberculosis H37Rv3377c encodes the diterpene cyclase for producing the halimane skeleton

Author keywords

[No Author keywords available]

Indexed keywords

5(6),13 HALIMADIEN 15 OL; BACTERIAL PROTEIN; DITERPENE; DITERPENE CYCLASE; DITERPENOID; GERANYLGERANYL PYROPHOSPHATE; HALIMANE; UNCLASSIFIED DRUG;

EID: 14944348414     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/b415346d     Document Type: Article
Times cited : (66)

References (25)
  • 5
    • 14944345506 scopus 로고    scopus 로고
    • Structural Genomics Corsortium
    • Mycobacterium tuberculosis Structural Genomics Corsortium. http:// www.doe-mbi.ucla.edu/TB.
    • Mycobacterium Tuberculosis
  • 10
    • 14944370147 scopus 로고    scopus 로고
    • note
    • The optimum catalytic pH and temperature for 1 → 2 were 6.5-7.5 and 15-25 °C, respectively; see ESI.
  • 11
    • 14944378708 scopus 로고    scopus 로고
    • note
    • Phosphatase inhibitors of cocktail 1 and 2 from SIGMA Co. were tested. Addition of the cocktail 1 led to the recoveries of GGOH and 2 from the hexane extract of the incubation mixture, but that of cocktail 2 resulted in no recovery of these alcohols, indicating that the cocktail 2 effectively inhibited the phosphatase activity of GGPP to GGOH and that of 4 to 2.
  • 15
    • 14944351574 scopus 로고    scopus 로고
    • note
    • Amino acid alignment of a few diterpene cyclases is given in the ESI.
  • 17
    • 0020482354 scopus 로고
    • No detectable amount of 2 was found in the hexane extract from the incubation mixture when 1 was incubated with the homogeneously purified Rv3377c enzyme, further supporting the proposal that this enzyme catalyses only the reaction of 1 → 4. To eliminate the diphosphate group of 4 to afford 2, acid phosphatase was added to the preincubation mixture of 1 with the purified Rv3377c enzyme, followed by further incubation; see, H. Fujii, T. Koyama and K. Ogura, Biochim. Biophys. Acta, 1982, 712, 716-718.
    • (1982) Biochim. Biophys. Acta , vol.712 , pp. 716-718
    • Fujii, H.1    Koyama, T.2    Ogura, K.3
  • 22
    • 14944381330 scopus 로고    scopus 로고
    • DNA Data Bank of Japan (DDBJ); http://www.ddbj.nig.ac.jp/.
  • 24
    • 14944354364 scopus 로고    scopus 로고
    • The Institute for Genomic Research (TIGR); http://www.tigr.org/.
  • 25
    • 4544346542 scopus 로고    scopus 로고
    • Very recently, Russell et al. suggested the horizontal gene transfer of Rv3377c and they constructed the transposon-inserted mutants that are defective in arrest of phagosome maturation, in which Rv3377c gene had been disrupted. See, K. Pethe, D. L. Swenson, S. Alonso, J. Anderson, C. Wang and D. G. Russell, Proc. Natl. Acad Sci. USA, 2004, 101, 13642-13647.
    • (2004) Proc. Natl. Acad Sci. USA , vol.101 , pp. 13642-13647
    • Pethe, K.1    Swenson, D.L.2    Alonso, S.3    Anderson, J.4    Wang, C.5    Russell, D.G.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.