Isolation and identification of the dominant Lactobacillus in gut and faeces of pigs using carbohydrate fermentation and 16S rDNA analysis

Journal of Bioscience and Bioengineering

Volumn 99, Issue 1, 2005, Pages 68-71

  Yin, Qingqiang ^a   Zheng, Qiuhong ^a  

^a HENAN AGRICULTURAL UNIVERSITY   (China)

Author keywords

16s rDNA; Carbohydrate fermentation; Lactobacillus; Pig gut

Indexed keywords

CARBOHYDRATES; CHEMICAL ANALYSIS; DNA; FERMENTATION; STRAIN;

GRAM STAINING; LACTOBACILLUS; MRS CULTURE;

BACTERIA;

CARBOHYDRATE; DNA 16S; RIBOSOME DNA;

ARTICLE; BACTERIUM IDENTIFICATION; CARBOHYDRATE METABOLISM; DNA SEQUENCE; FECES ANALYSIS; FERMENTATION; INTESTINE; LACTOBACILLUS; NONHUMAN; POLYMERASE CHAIN REACTION; SWINE;

LACTOBACILLUS; LACTOBACILLUS RUMINIS; SUS SCROFA;

EID: 14644440561     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.99.68     Document Type: Article

References (20)

1. Shahani, K. M., Vakil, J. R., and Kilara, A.: Natural antibiotic activity of Lactobacillus acidophilus and L. bulgaricus. 2. Isolation of acidophilin from L. acidophilus. Cult. Dairy Prod. J., 12, 8 (1977).
2. Fuller, R.: Probiotics. J. Appl. Bacteriol., Symposium Supplement, 1S-7S (1986).
3. Zani, J. L.: Effect of probiotic CenBiot on the control of diarrhoea and feed efficiency in pigs. J. Appl. Microbiol., 84, 68-71 (1998).
4. Garriga, M.: Selection of Lactobacilli for chicken probiotic adjuncts. J. Appl. Microbiol., 84, 125-132 (1998).
5. Pollmann, D. S., Danielson, D. M., and Poe, E. R.: Effects of microbial feed additives on performance of starter and growing-finishing pigs. J. Anim. Sci., 51, 577-581 (1980).
6. McDonald, M.: Probiotics why the interest? Aust. Poult., 9, 3-5 (1998).
7. Christensen, N.: Antibiotic-free went chicken. Poult. Digest., 6, 21-23 (2000).
8. Collins, M. D., Rodrigues, U., Aguirre, M., Farrow, J. A. E., Martinez-Murcia, A., Philips, B. A., Williams, A. M., and Wallbanks, S.: Phylogenetic analysis of the genus lactobacillus and related lactic acid bacteria as determined by reverse transcriptase sequencing of 16S rDNA. FEMS Microbiol. Lett., 77, 5-12 (1991).
9. Ogimoto, K. and Imai, S.: ATLAS of rumen microbiology, p. 118. Japanese Scientific Societies Press, Tokyo (1981).
10. Hensiek, R., Krupp, G., and Stackebrandt, E.: Development of diagnostic oligonucleotide probes for four Lactobacillus species occurring in the intestinal tract. Syst. Appl. Bacteriol., 15, 123-128 (1992).
11. Caldwell, D. R. and Bryant, M. P.: Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Appl. Microbiol., 14, 794 (1966).
12. Man, J. C., Rogosa, M., and Sharpe, M. E.: A medium for the cultivation of lactobacilli. J. Appl. Bacteriol., 23, 130-135 (1960).
13. Zoetendal, E. G., Akkermans, A. D. L., and de Vos, W. M.: Temperature gradient gel host-specific communities of active bacteria. Appl. Environ. Microbiol., 64, 3854-3859 (1998).
14. Heilig, H. G. H. J., Zoetendal, E. G., Vaughan, E. E., Marteau, P., Akkermans, A. D. L., and de Vos, V. W.: Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplication of 16S ribosomal DNA. Appl. Environ. Microbiol., 68, 114-123 (2002).
15. Dojka, M. A., Hugenholtz, P., Haack, S. K., and Pace, N. R.: Microbial diversity in a hydrocarbon and chlorinated-solvent-contaminated aquifer undergoing intrinsic bioremediation. Appl. Environ. Microbiol., 64, 3869-3877 (1998).
16. Tannock, G. W.: The microecology of lactobacilli inhabiting the gastrointestinal tract. Adv. Microb. Ecol., 11, 147-171 (1990).
17. Fuller, R.: Probiotics, p. 17-271. Chapman and Hall, London (1992).
18. Smith, S. I., Olukoya, D. K., Bakare, S., and Olayiwola, W. A.: Isolation and characterization of Lactobacillus species from pig faeces. Biomed. Lett., 59, 185-190 (1999).
19. Leser, T. D., Amenuvor, J. Z., Jensen, T. K., Lindecrona, R. H., Boye, M., and Moller, K.: Culture-independent analysis of gut bacteria: the pig gastrointerstinal tract microbiota revisited. Appl. Environ. Microbiol., 68, 673-690 (2002).
20. Al Jassim, R. A. M.: Lactobacillus ruminis is a predominant lactic acid producing bacterium in the caecum and rectum of the pig. Lett. Appl. Microbiol., 37, 213-217 (2003).