In vitro evolutionary thermostabilization of congerin II: A limited reproduction of natural protein evolution by artificial selection pressure

Journal of Molecular Biology

Volumn 347, Issue 2, 2005, Pages 385-397

  Shionyu Mitsuyama, Clara ^a   Ito, Yoshimaro ^b   Konno, Ayumu ^b   Miwa, Yukiko ^c   Ogawa, Tomohisa ^b   Muramoto, Koji ^b   Shirai, Tsuyoshi ^a  

^a BIOMOL ENG RESEARCH INSTITUTE   (Japan)

^b TOHOKU UNIVERSITY   (Japan)

^c NARA INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Crystal structure; Galectin; Random mutagenesis; Thermostability

Indexed keywords

CONGERIN I; CONGERIN II; GALECTIN; MUTANT PROTEIN; SERINE; THREONINE; TYROSINE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ARTIFICIAL SELECTION; CRYSTAL STRUCTURE; EEL; ESCHERICHIA COLI; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN STABILITY; THERMOSTABILITY;

EID: 14644386787     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2005.01.027     Document Type: Article

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