Synthesis of novel biodegradable quaternary amine-based cross-linked poly(β-amino ester) and its self-assembly/disassembly with plasmid DNA

Bulletin of the Korean Chemical Society

Volumn 26, Issue 1, 2005, Pages 175-177

  Kim, Tae Il ^a   Seo, Hyo Jung ^a   Baek, Jung Un ^a   Park, Jae Hun ^b   Park, Jong Sang ^a  

^a Seoul National University   (South Korea)

^b Korea Minjok Leadership Academy   (South Korea)

Author keywords

Biodegradability; Cross linked polymer; Gene delivery; Quaternary amine

Indexed keywords

AMINE; DRUG CARRIER; ESTER DERIVATIVE; PLASMID DNA; POLYMER; QUATERNARY AMINE BASED CROSS LINKED POLY(BETA AMINO ESTER); UNCLASSIFIED DRUG;

AQUEOUS SOLUTION; ARTICLE; BIODEGRADABILITY; CHEMICAL BOND; CHEMICAL REACTION; CROSS LINKING; DISSOCIATION; DISULFIDE BOND; DNA DRUG COMPLEX; DRUG DEGRADATION; DRUG DNA INTERACTION; DRUG STABILITY; DRUG STRUCTURE; DRUG SYNTHESIS; ELECTRIC POTENTIAL; GENE DELIVERY SYSTEM; GENETIC TRANSFECTION; IN VITRO STUDY; LIGHT SCATTERING; METHYLATION; MICHAEL ADDITION; MOLECULAR WEIGHT; NONHUMAN; POLYMERIZATION; PROTON NUCLEAR MAGNETIC RESONANCE; REACTION ANALYSIS;

EID: 14544274114     PISSN: 02532964     EISSN: None     Source Type: Journal    
DOI: 10.5012/bkcs.2005.26.1.175     Document Type: Article

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11. 3) = 3.08 (s).
12. The SEC system consisted of a P680 HPLC pump from Dionex Corp. (Sunnyvale, CA). Polymer sample was detected by a 3-angle laser light scattering detector (miniDAWN® Tristar™, 30 mW GaAs laser, 690 nm) and an interferometric refractometer (Optilab DSP™) from Wyatt Technologies (Santa Barbara, CA). DMF was used as an eluent after being filtered through a 0.22 μn filter and degassed before use. A Styragel® HR 3 column from Waters (Milford, MA) was used.
13. (a) 2 mL of polyplex solutions containing 5 μg of DNA were prepared at various weight ratios ranging from 1 to 20. After 30 min incubation, polyplex sizes were measured using a Zetasizer 3000HS (5 mW HeNe laser, 633 nm, Malvern Instruments, UK). Scattered light was detected at a 90° angle.
14. (b) Polyplex solutions were prepared in same method as above. Polyplex solutions were diluted to 10 mL of the final volume prior to measurements. Zeta-potential measurements were carried out using a Zetasizer 3000HS at 25 °C.
15. Polyplexes at weight ratios of 5 and 20, were prepared in Hepes buffered saline (HBS, 20 mM Hepes, 150 mM NaCl, pH 7.4). The samples were collected for assay at various times ranging from the 1st to the 5th day, electrophoresed on a 0.7% (w/v) agarose gel and then stained in a 1% (w/v) ethidium bromide solution.