A DNA enzyme that mimics the first step of RNA splicing

Nature Structural and Molecular Biology

Volumn 11, Issue 3, 2004, Pages 270-274

  Coppins, Rebecca L ^a   Silverman, Scott K ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE; DEOXYRIBOZYME; DNA; HYDROXYL GROUP; NUCLEIC ACID; PHOSPHATE; RIBOZYME; RNA;

ARTICLE; CATALYSIS; ENZYME ANALYSIS; ENZYME MECHANISM; IN VITRO STUDY; IN VIVO STUDY; PRIORITY JOURNAL; PROTEIN FUNCTION; RNA SPLICING;

ADENOSINE; BASE SEQUENCE; DNA, CATALYTIC; KINETICS; MOLECULAR MIMICRY; NUCLEIC ACID CONFORMATION; RNA; RNA SPLICING;

EID: 1442286924     PISSN: 15459993     EISSN: None     Source Type: Journal    
DOI: 10.1038/nsmb727     Document Type: Article

References (28)

1. Cech, T.R. The ribosome is a ribozyme. Science 289, 878-879 (2000).
2. Collins, C.A. & Guthrie, C. The question remains: is the spliceosome a ribozyme? Nat. Struct. Biol. 7, 850-854 (2000).
3. Michel, F. & Ferat, J.L. Structure and activities of group II introns. Annu. Rev. Biochem. 64, 435-461 (1995).
4. Peebles, C.L. et al. A self-splicing RNA excises an intron lariat. Cell 44, 213-223 (1986).
5. Burge, C.B., Tuschl, T. & Sharp, P.A. Splicing of precursors to mRNAs by the spliceosomes. In The RNA World (eds. Gesteland, R.F., Cech, T.R. & Atkins, J.F.) 525-560 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1999).
6. Valadkhan, S. & Manley, J.L. Splicing-related catalysis by protein-free snRNAs. Nature 413, 701-707 (2001).
7. Breaker, R.R. In vitro selection of catalytic polynucleotides. Chem. Rev. 97, 371-390 (1997).
8. Wilson, D.S. & Szostak, J.W. In vitroselection of functional nucleic acids. Annu. Rev. Biochem. 68, 611-647 (1999).
9. Soumillion, P. & Fastrez, J. Novel concepts for selection of catalytic activity. Curr. Opin. Biotechnol. 12, 387-394 (2001).
10. Dower, W.J. & Mattheakis, L.C. In vitro selection as a powerful tool for the applied evolution of proteins and peptides. Curr. Opin. Chem. Biol. 6, 390-398 (2002).
11. Breaker, R.R. & Joyce, G.F. A DNA enzyme that cleaves RNA. Chem. Biol. 1, 223-229 (1994).
12. Breaker, R.R. Making catalytic DNAs. Science 290, 2095-2096 (2000).
13. Lu, Y. DNAzymes-A new class of enzymes with promise in biochemical, pharmaceutical, and biotechnological applications. Chem. Eur. J. 8, 4589-4596 (2002).
14. Emilsson, G.M. & Breaker, R.R. Deoxyribozymes: new activities and new applications. Cell. Mol. Life Sci. 59, 596-607 (2002).
15. Flynn-Charlebois, A. et al. Deoxyribozymes with 2′-5′ RNA ligase activity. J. Am. Chem. Soc. 125, 2444-2454 (2003).
16. Bartel, D.P. & Szostak, J.W. Isolation of new ribozymes from a large pool of random sequences. Science 261, 1411-1418 (1993).
17. Ekland, E.H. & Bartel, D.P. RNA-catalysed RNA polymerization using nucleos de triphosphates. Nature 382, 373-376 (1996).
18. Wang, Y. & Silverman, S.K. Deoxyribozymes that synthesize branched and lariat RNA. J. Am. Chem. Soc. 125, 6880-6881 (2003).
19. Wang, Y. & Silverman, S.K. Characterization of deoxyribozymes that synthesize branched RNA. Biochemistry 42, 15252-15263 (2003).
20. Nam, K. et al. Yeast lariat debranching enzyme. Substrate and sequence specificity. J. Biol. Chem. 269, 20613-20621 (1994).
21. Hager, A.J. & Szostak, J.W. Isolation of novel ribozymes that ligate AMP-activated RNA substrates. Chem. Biol. 4, 607-617 (1997).
22. Robertson, M.P. & Ellington, A.D. In vitro selection of an allosteric ribozyme that transduces analytes to amplicons. Nat. Biotechnol. 17, 62-66 (1999).
23. Gilbert, W. The RNA world. Nature 319, 618 (1986).
24. Joyce, G.F. & Orgel, L.E. Prospects for understanding the origin of the RNA world. In The RNA World (eds. Gesteland, R.F., Cech, T.R. & Atkins, J.F.) 49-77 (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 1999).
25. Eschenmoser, A. Chemical etiology of nucleic acid structure. Science 284, 2118-2124 (1999).
26. Schöning, K. et al. Chemical etiology of nucleic acid structure: the α-threofuranosyl-(3′→2±) oligonucleotide system. Science 290, 1347-1351 (2000).
27. Milligan, J.F., Groebe, D.R., Witherell, G.W. & Uhlenbeck, O.C. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 15, 8783-8798 (1987).
28. Silverman, S.K. Practical and general synthesis of 5′-adenylated RNA (5′-AppRna). RNA (in the press).