Legionelil Effectors That Promote Nonlytic Release from Protozoa

Science

Volumn 303, Issue 5662, 2004, Pages 1358-1361

  Chen, John ^a   De Felipe, Karim Suwwan ^a   Clarke, Margaret ^b   Lu, Hao ^b   Anderson, O Roger ^c   Segal, Gil ^d   Shuman, Howard A ^a  

^a Columbia University ^*  (United States)

^b OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

^c Department of Earth and Environmental Sciences   (United States)

^d TEL AVIV UNIVERSITY   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; CELLS; DISEASES; MUTAGENESIS; PATHOLOGY;

PATHOGENS;

PROTOZOA;

LEPA PROTEIN; LEPB PROTEIN; POLYPEPTIDE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CYCLIC AMP; HYBRID PROTEIN; LEPA PROTEIN, LEGIONELLA PNEUMOPHILA; LEPB PROTEIN, LEGIONELLA PNEUMOPHILA;

MEDICINE;

ARTICLE; BACTERIAL GENOME; BACTERIAL REPRODUCTION; CELL VACUOLE; EXOCYTOSIS; HOST CELL; LEGIONELLA PNEUMOPHILA; LEGIONNAIRE DISEASE; MAMMAL; OPEN READING FRAME; PRIORITY JOURNAL; PROTOZOON; ACANTHAMOEBA; ANIMAL; BACTERIAL COUNT; CELL LINE; DICTYOSTELIUM; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; LYSOSOME; MACROPHAGE; METABOLISM; MICROBIOLOGY; MUTATION; PATHOGENICITY; PHAGOSOME; PHYSIOLOGY; ULTRASTRUCTURE;

BACTERIA (MICROORGANISMS); LEGIONELLA; LEGIONELLA PNEUMOPHILA; MAMMALIA; NEGIBACTERIA; PROTOZOA;

ACANTHAMOEBA; ANIMALS; BACTERIAL PROTEINS; CELL LINE; COLONY COUNT, MICROBIAL; CYCLIC AMP; DICTYOSTELIUM; EXOCYTOSIS; GENOME, BACTERIAL; HUMANS; LEGIONELLA PNEUMOPHILA; LYSOSOMES; MACROPHAGES; MUTATION; OPEN READING FRAMES; PHAGOSOMES; RECOMBINANT FUSION PROTEINS; VACUOLES;

EID: 1442281243     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1094226     Document Type: Article

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28. We thank I. Rosenshine and I. Nisan of the Hebrew University Medical School in Ein Kerem, Israel, for plasmid pEX-CyaA and for helping us initiate the work with the CyaA cyclase. We thank R. Kessin, G. Otto, and M. Wu for help with Dictyostelium and helpful comments on the manuscript. We acknowledge the assistance of M. Reyes for plasmid construction, and the use of microscopy equipment in the Oklahoma Medical Research Foundation imaging facility. This work was supported by grants: NIH-R01 AI23549 (H.A.S.); NSF MCB-0344541and OCAST-HR01-024 (M.C.); grant Nn. 1999011 from the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel (G.S.).