Comparison of different methods to assess the cytotoxic effects of cytosine deaminase and thymidine kinase gene therapy

Cancer Gene Therapy

Volumn 11, Issue 3, 2004, Pages 208-214

  Corban Wilhelm, Heike ^a   Ehemann, Volker ^b   Becker, Gabriele ^a   Greulich, Ditmar ^a   Braun, Klaus ^a   Debus, Jürgen ^a  

^a Clin Coop U for Dermato Oncology   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

Cell death; Cytosine deaminase; Thymidine kinase

Indexed keywords

CYTOSINE DEAMINASE; FLUCYTOSINE; GANCICLOVIR; THYMIDINE KINASE;

ANIMAL CELL; ARTICLE; CELL CYCLE; CELL CYCLE S PHASE; CELL DEATH; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DOSE RESPONSE; ESCHERICHIA COLI; GENE THERAPY; GENOTOXICITY; HERPES SIMPLEX VIRUS 1; MALE; NONHUMAN; PRIORITY JOURNAL; PROSTATE CARCINOMA; RAT; SUICIDE GENE;

ANIMALS; APOPTOSIS; CELL CYCLE; CELL LINE, TUMOR; CELL SURVIVAL; CYTOSINE DEAMINASE; ESCHERICHIA COLI; FLUCYTOSINE; GANCICLOVIR; GENE THERAPY; GENE TRANSFER TECHNIQUES; GENES, TRANSGENIC, SUICIDE; HERPESVIRUS 1, HUMAN; MALE; PROSTATIC NEOPLASMS; RATS; RECOMBINANT FUSION PROTEINS; TETRAZOLIUM SALTS; THIAZOLES; THYMIDINE KINASE; TOXICITY TESTS; TUMOR STEM CELL ASSAY;

ANIMALIA; ESCHERICHIA COLI; HUMAN HERPESVIRUS 1; SIMPLEXVIRUS;

EID: 1442275333     PISSN: 09291903     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cgt.7700667     Document Type: Article

References (29)

1. Hamzeh FM, Lietman PS. Intranuclear accumulation of subgenomic noninfectious human cytomegalovirus DNA in infected cells in the presence of ganciclovir. Antimicrob Agents Chemother. 1991;35:1818-1823.
2. Haynes P, Lambert TR, Mitchell ID. Comparative in-vivo genotoxicity of antiviral nucleoside analogues; penciclovir, acyclovir, ganciclovir and the xanthine analogue, caffeine, in the mouse bone marrow micronucleus assay. Mutat Res. 1996;369:65-74.
3. Heidelberger C, Chaudhuri NK, Danneberg P, et al. Fluorinated pyrimidines, a new class of tumour-inhibitory compounds. Nature. 1957;179:663-666.
4. Willmore E, Durkacz BW. Cytotoxic mechanisms of 5-fluoropyrimidines. Relationships with poly(ADP-ribose) polymerase activity, DNA strand breakage and incorporation into nucleic acids. Biochem Pharmacol. 1993;46:205-211.
5. Weckbecker G. Biochemical pharmacology and analysis of fluoropyrimidines alone and in combination with modulators. Pharmacol Ther. 1991;50:367-424.
6. Daher GC, Harris BE, Diasio RB. Metabolism of pyrimidine analogues and their nucleosides. Pharmacol Ther. 1990;48:189-222.
7. Inaba M, Mitsuhashi J. Flow cytometric analysis of cell-killing actions of 5-fluorouracil in human colorectal cancer cells. Oncol Res. 1994;6:303-309.
8. Barltrop JA, Owen TC, Cory AH, Cory JG. 5-(3-Carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)-3-(4-sulfophenyl) tetrazolium, inner salt (MTS) and related analogs of 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) reducing to purple water-soluble formazans as cell-viability indicators. Bioorg Med Chem Lett. 1991;1:611.
9. Freshney RI. Culture of Animal Cells - A Manual of Basic Technique 4th edn. Wiley-Liss; New York, U.S.A., 2000.
10. Ehmann GL, McLean TI, Bachenheimer SL. Herpes simplex virus type 1 infection imposes a G(1)/S block in asynchronously growing cells and prevents G(1) entry in quiescent cells. Virology. 2000;267:335-349.
11. Grem JL. 5-Fluorouracil: forty-plus and still ticking. A review of its preclinical and clinical development. Investig New Drugs. 2000;18:299-313.
12. Beltinger C, Fulda S, Kammertoens T, Uckert W, Debatin KM. Mitochondrial amplification of death signals determines thymidine kinase/ ganciclovir-triggered activation of apoptosis. Cancer Res. 2000;60:3212-3217.
13. Beltinger C, Fulda S, Kammertoens T, Meyer E, Uckert W, Debatin KM. Herpes simplex virus thymidine kinase/ganciclovir-induced apoptosis involves ligand-independent death receptor aggregation and activation of caspases. Proc Natl Acad Sci USA. 1999;96:8699-8704,
14. Wang ZH, Samuels S, Gama-Sosa MA, Kolodny EH. 5-Fluorocytosine-mediated apoptosis and DNA damage in glioma cells engineered to express cytosine deaminase and their enhancement with interferon. J Neurooncol. 1998;36:219-229.
15. Rubsam LZ, Davidson BL, Shewach DS. Superior cytotoxicity with ganciclovir compared with acyclovir and 1-beta-D-arabinofuranosylthymine in herpes simplex virus-thymidine kinase-expressing cells: a novel paradigm for cell killing. Cancer Res. 1998;58:3873-3882.
16. Isaacs JT, Isaacs WB, Feitz WF, Scheres J. Establishment and characterization of seven Dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers. Prostate. 1986;9:261-281.
17. Rogulski KR, Kim JH, Kim SH, Freytag SO. Glioma cells transduced with an Escherichia coli CD/HSV-1 TK fusion gene exhibit enhanced metabolic suicide and radiosensitivity. Hum Gene Ther. 1997;8:73-85.
18. Haack K, Moebius U, Knebel-Doeberitz MV, Herfarth C, Schackert HK, Gebert JF. Detection of cytosine deaminase in genetically modified tumor cells by specific antibodies. Hum Gene Ther. 1997;8:1395-1401.
19. Otto FJ. High-resolution analysis of nuclear DNA employing the fluorochrome DAPI. Methods Cell Biol. 1994;41:211-217.
20. Foglieni C, Meoni C, Davalli AM. Fluorescent dyes for cell viability: an application on prefixed conditions. Histochem Cell Biol. 2001;115:223-229.
21. Cory AH, Owen TC, Barltrop JA, Cory JG. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun. 1991;3:207-212.
22. Wu XP, Dolnick BJ. 5-Fluorouracil alters dihydrofolate reductase pre-mRNA splicing as determined by quantitative polymerase chain reaction. Mol Pharmacol. 1993;44:22-29.
23. Corban-Wilhelm H, Hull WE, Becker G, Bauder-Wust U, Greulich D, Debus J. Cytosine deaminase and thymidine kinase gene therapy in a Dunning rat prostate tumour model: absence of bystander effects and characterisation of 5-fluorocytosine metabolism with (19)F-NMR spectroscopy. Gene Therapy. 2002;9:1564-1575.
24. Dive C, Gregory CD, Phipps DJ, Evans DL, Milner AE, Wyllie AH. Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochim Biophys Acta. 1992;1133:275-285.
25. Huschtscha LI, Jeitner TM, Andersson CE, Bartier WA, Tattersall MH. Identification of apoptotic and necrotic human leukemic cells by flow cytometry. Exp Cell Res. 1994;212:161-165,
26. Yoshimura I, Suzuki S, Tadakuma T, Hayakawa M. Suicide gene therapy on LNCaP human prostate cancer cells. Int J Urol. 2001;8:S5-S8.
27. Calabresi P, Chabner BA. Chemotherapy of neoplastic diseases. In: Hardman JG, Limbird LE, Molinoff PB, Ruddon RW, Gilman AG, eds. Goodman & Gilman's "The Pharmacological Basis of Therapeutics". New York: McGraw-Hill; 1995:1225-1287.
28. Thust R, Schacke M, Wutzler P. Cytogenetic genotoxicity of antiherpes virostatics in Chinese hamster V79-E cells. I. Purine nucleoside analogues. Antiviral Res. 1996;31:105-113.
29. Aghi M, Kramm CM, Chou TC, Breakefield XO, Chiocca EA. Synergistic anticancer effects of ganciclovir/thymidine kinase and 5-fluorocytosine/ cytosine deaminase gene therapies. J Natl Cancer Inst. 1998;90:370-380.