Isolation and enrichment of murine spermatogonial stem cells using rhodamine 123 mitochondrial dye

Biology of Reproduction

Volumn 72, Issue 3, 2005, Pages 767-771

  Lo, Kirk C ^a,b   Brugh III, Victor M ^b,c   Parker, Michele ^b   Lamb, Dolores J ^b  

^a UNIVERSITY OF TORONTO   (Canada)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

^c Eastern Virginia School of Medicine   (United States)

Author keywords

Male reproductive tract; Sperm; Spermatogenesis; Testis

Indexed keywords

FLUORESCENT DYE; RHODAMINE 123;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL POPULATION; CONTROLLED STUDY; CRYPTORCHISM; FLOW CYTOMETRY; MALE; METHODOLOGY; MITOCHONDRION; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPERMATOGONIUM; STEM CELL; TESTIS CELL;

ANIMALS; CELL SEPARATION; FLOW CYTOMETRY; FLUORESCENT DYES; GERM CELLS; MALE; MICE; RHODAMINE 123; SPERMATOGONIA; STEM CELL TRANSPLANTATION; STEM CELLS; TESTIS;

ANIMALIA; MURINAE;

EID: 14044266304     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod.104.033464     Document Type: Article

References (23)

1. Brinster RL, Zimmermann JW. Spermatogenesis following male germ-cell transplantation. Proc Natl Acad Sci U S A 1994; 91:11298-11302.
2. Brinster RL, Avarbock MR. Germline transmission of donor haplotype following spermatogonial transplantation. Proc Natl Acad Sci U S A 1994; 91:11303-11307.
3. Shinohara T, Avarbock MR, Brinster RL. Functional analysis of spermatogonial stem cells in Steel and cryptorchid infertile mouse models. Dev Biol 2000; 220:401-411.
4. Shinohara T, Orwig KE, Avarbock MR, Brinster RL. Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells. Proc Natl Acad Sci U S A 2000; 97:8346-8351.
5. Giuili G, Tomljenovic A, Labrecque N, Oulad-Abdelghani M, Rassoulzadegan M, Cuzin F. Murine spermatogonial stem cells: targeted transgene expression and purification in an active state. EMBO Rep 2002; 3:753-759.
6. Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med 1996; 183:1797-1806.
7. Gussoni E, Soneoka Y, Strickland CD, Buzney EA, Khan MK, Flint AF, Kunkel LM, Mulligan RC. Dystrophin expression in the mdx mouse restored by stem cell transplantation. Nature 1999; 401:390-394.
8. Hulspas R, Quesenberry PJ. Characterization of neurosphere cell phenotypes by flow cytometry. Cytometry 2000; 40:245-250.
9. Gottesman MM, Fojo T, Bates SE. Multidrug resistance in cancer: role of ATP-dependent transporters. Nat Rev Cancer 2002; 2:48-58.
10. Bunting KD. ABC transporters as phenotypic markers and functional regulators of stem cells. Stem Cells 2002; 20:11-20.
11. Lassalle B, Bastos H, Louis JP, Riou L, Testart J, Dutrillaux B, Fouchet P, Allemand I. 'Side population' cells in adult mouse testis express Bcrp1 gene and are enriched in spermatogonia and germinal stem cells. Development 2004; 131:479-487.
12. Falciatori I, Borsellino G, Haliassos N, Boitani C, Corallini S, Battistini L, Bernardi G, Stefanini M, Vicini E. Identification and enrichment of spermatogonial stem cells displaying side-population phenotype in immature mouse testis. FASEB J 2004; 18:376-378.
13. Kubota H, Avarbock MR, Brinster RL. Spermatogonial stem cells share some, but not all, phenotypic and functional characteristics with other stem cells. Proc Natl Acad Sci U S A 2003; 100:6487-6492.
14. Spangrude GJ, Johnson GR. Resting and activated subsets of mouse multipotent hematopoietic stem cells. Proc Natl Acad Sci U S A 1990; 87:7433-7437.
15. Bertoncello I, Hodgson GS, Bradley TR. Multiparameter analysis of transplantable hemopoietic stem cells: I. The separation and enrichment of stem cells homing to marrow and spleen on the basis of rhodamine-123 fluorescence. Exp Hematol 1985; 13:999-1006.
16. Mulder AH, Visser JW. Separation and functional analysis of bone marrow cells separated by rhodamine 123 fluorescence. Exp Hematol 1987; 15:99-104.
17. Leemhuis T, Yoder MC, Grigsby S, Aguero B, Eder P, Srour EF. Isolation of primitive human bone marrow hematopoietic progenitor cells using Hoechst 33342 and rhodamine 123. Exp Hematol 1996; 24:1215-1224.
18. Radley JM, Ellis S, Palatsides M, Williams B, Bertoncello I. Ultrastructure of primitive hematopoietic stem cells isolated using probes of functional status. Exp Hematol 1999; 27:365-369.
19. Uchida N, Dykstra B, Lyons KJ, Leung FY, Eaves CJ. Different in vivo repopulating activities of purified hematopoietic stem cells before and after being stimulated to divide in vitro with the same kinetics. Exp Hematol 2003; 31:1338-1347.
20. Wolf NS, Kone A, Priestley GV, Bartelmez SH. In vivo and in vitro characterization of long-term repopulating primitive hematopoietic cells isolated by sequential Hoechst 33342-rhodamine 123 FACS selection. Exp Hematol 1993; 21:614-622.
21. Ogawa T, Arechaga JM, Avarbock MR, Brinster RL. Transplantation of testis germinal cells into mouse seminiferous tubules. Int J Dev Biol 1997; 41:111-122.
22. Nagano M, Avarbock MR, Brinster RL. Pattern and kinetics of mouse donor spermatogonial stem cell colonization in recipient testes. Biol Reprod 1999; 60:1429-1436.
23. Dobrinski I, Ogawa T, Avarbock MR, Brinster RL. Computer assisted image analysis to assess colonization of recipient seminiferous tubules by spermatogonial stem cells from transgenic donor mice. Mol Reprod Dev 1999; 53:142-148.