Improving consistency of cell-based assays by using division-arrested cells

Assay and Drug Development Technologies

Volumn 3, Issue 1, 2005, Pages 7-15

  Fursov, Natalie ^a   Cong, Mei ^a   Federici, Mark ^b   Platchek, Michael ^a   Haytko, Peter ^a   Tacke, Roland ^b   Livelli, Thomas ^b   Zhong, Zhong ^b  

^a Cell and Molecular Technologies Inc   (United States)

^b Cell and Molecular Technologies   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CASPASE 3; CASPASE 7; MITOMYCIN C; REAGENT;

APOPTOSIS; ARTICLE; CALCIUM CELL LEVEL; CALCIUM MOBILIZATION; CALCIUM TRANSPORT; CELL ASSAY; CELL CULTURE; CELL CYCLE; CELL DIVISION; CELL GROWTH; CELL STRAIN HEK293; CELL TYPE; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; REPORTER GENE; ROBOTICS; SIGNAL TRANSDUCTION;

ANIMALS; BIOLOGICAL ASSAY; CELL CULTURE TECHNIQUES; CELL CYCLE; CELL LINE; CRYOPRESERVATION; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG DESIGN; HUMANS; KIDNEY; MICE; MITOMYCIN; NIH 3T3 CELLS; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; ROBOTICS; SENSITIVITY AND SPECIFICITY;

EID: 14044263248     PISSN: 1540658X     EISSN: None     Source Type: Journal    
DOI: 10.1089/adt.2005.3.7     Document Type: Article

References (18)

1. Johnston PA, Johnston PA: Cellular platforms for HTS: Three case studies. Drug Discov Today 2002;7:353-363.
2. Verweij J, den Hartigh J, Pinedo HM: In: Antitumor Antibiotics. Chabner BA, Collins JM, (eds.), pp. 382-396. Lippincott, Philadelphia, 1990.
3. Goodman & Gilman's The Pharmacological Basis of Therapeutics, 10th ed., p. 1431. McGraw-Hill, New York, 2001.
4. Szybalski W, Iyer VN: Crosslinking of DNA by enzymatically or chemically activated mitomycins and porfiromycins, bifunctionally "alkylating" antibiotics. Fed Proc 1964;23:946-957.
5. Bizanek R, Chowdary D, Arai H, Kasai M, Hughes CS, Sartorelli AC, Rockwell S, Tomasz M: Adducts of mitomycin C and DNA in EMT6 mouse mammary tumor cells: Effects of hypoxia and dicumarol on adduct patterns. Cancer Res 1993;53:5127-5134.
6. Palom Y, Belcourt MF, Musser SM, Sartorelli AC, Rockwell S, Tomasz M: Structure of adduct X, the last unknown of the six major DNA adducts of mitomycin C formed in EMT6 mouse mammary tumor cells. Chem Res Toxicol 2000;13:479-488.
7. 32P-postlabeling: time course for formation and removal of adducts and biochemical modulation. Cancer Res 1998;58:453-461.
8. Warren AJ, Mustra DJ, Hamilton JW: Detection of mitomycin C-DNA adducts in human breast cancer cells grown in culture, as xenografted tumors in nude mice, and in biopsies of human breast cancer patient tumors as determined by (32)P-postlabeling. Clin Cancer Res 2001;7:1033-1042.
9. Pan SS, Yu F, Hipsher C: Enzymatic and pH modulation of mitomycin C-induced DNA damage in mitomycin C-resistant HCT 116 human colon cancer cells. Mol Pharmacol 1993;43:870-877.
10. Abbas T, Olivier M, Lopez J, Houser S, Xiao G, Kumar GS, Tomasz M, Bargonetti J: Differential activation of p53 by the various adducts of mitomycin C. J Biol Chem 2002;277:40513-40519.
11. Federici M, Tacke R, Haytko P, Zachwieja S, Power SC, Cong M, Livelli T, Zhong Z: From target to screen in two weeks: NF-κB reporter assay with transient target expression [abstract P08046]. Presenetd at the 9th Society for Biomolecular Screening, Annual Conference and Exhibition, Portland, OR, September 22, 2003.
12. Julius D, Livelli T, Jessell T, Axel R: Ectopic expression of the serotonin 1c receptor and the triggering of malignant transformation. Science 1989;244:1057-1062.
13. Goodwin BL, Solomonson LP, Eichler DC: Argininosuccinate synthase expression is required to maintain nitric oxide production and cell viability in aortic endothelial cells. J Biol Chem 2004;279:18353-18360.
14. Lin PF, Blair W, Wang T, Spicer T, Guo Q, Zhou N, Gong YF, Wang HG, Rose R, Yamanaka G, Robinson B, Li CB, Fridell R, Deminie C, Demers G, Yang Z, Zadjura L, Meanwell N, Colonno R: A small molecule HIV-1 inhibitor that targets the HIV-1 envelope and inhibits CD4 receptor binding. Proc Natl Acad Sci U S A 2003;100:11013-11018.
15. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999;4: 67-73.
16. Chandrasekaran V, Maunder P, Haskins J, Fursov N, Cong M, Zhong Z: The use of division arrested cells improves the robustness of high content screening assays. Presented at the 10th Society for Biomolecular Screening, Annual Conference and Exhibition, Orlando, FL, September 22, 2004.
17. Fursov N, Cong M, Federici M, Tacke R, Worzella T, Larsen B, Cowan C, Livelli T, Zhong Z: Improving consistency of cell-based assays by using division-arrested cell reagents. Presented at the IBC's Drug Discovery Technology® World Congress, Boston, MA, August 10, 2004.
18. Grove GN, Benway M, Alderman EM, Cowan C, Cong M, Federichi M, Livelli T, Zhong Z: Automation of apoptosis and NF-κB reporter gene assays using division-arrested HEK293 cells. Presented at the LabAutomation 2004 Conference, San Jose, CA, February 2, 2004.