Amiloride uptake and toxicity in fission yeast are caused by the pyridoxine transporter encoded by bsu1+ (car1+)

Eukaryotic Cell

Volumn 4, Issue 2, 2005, Pages 319-326

  Stolz, Jürgen ^a   Wöhrmann, Heike J P ^a   Vogl, Christian ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMILORIDE; BSU1 PROTEIN, S POMBE; CARRIER PROTEIN; DIURETIC AGENT; HYBRID PROTEIN; PYRIDOXINE; SCHIZOSACCHAROMYCES POMBE PROTEIN; THIAMINE;

ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; GENETIC COMPLEMENTATION; GENETICS; HUMAN; METABOLISM; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES;

AMILORIDE; DIURETICS; GENETIC COMPLEMENTATION TEST; HUMANS; MEMBRANE TRANSPORT PROTEINS; MOLECULAR STRUCTURE; PYRIDOXINE; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; SCHIZOSACCHAROMYCES POMBE PROTEINS; SUBSTRATE SPECIFICITY; THIAMINE;

SACCHAROMYCES CEREVISIAE; SACCHAROMYCES POMBE; SCHIZOSACCHAROMYCES POMBE;

EID: 13844314453     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.4.2.319-326.2005     Document Type: Article

References (51)

1. Agatep, R., R. D. Kirkpatrick, D. L. Parchaliuk, R. A. Woods, and R. D. Gietz. 1998. Transformation of Saccharomyces cerevisiae by the lithium acetate/single-stranded carrier DNA/polyethylene glycol (LiAc/ss-DNA/PEG) protocol. Technical Tips Online (http://tto.trends.com).
2. Bähler, J., J. Q. Wu, M. S. Longtine, N. G. Shah, A. McKenzie III, A. B. Steever, A. Wach, P. Philippsen, and J. R. Pringle. 1998. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14:943-951.
3. Bauer, J. A., E. M. Bennett, T. P. Begley, and S. E. Ealick. 2004. Three-dimensional structure of YaaE from Bacillus subtilis, a glutaminase implicated in pyridoxal-5′-phosphate biosynthesis. J. Biol. Chem. 279:2704-2711.
4. Bean, L. E., W. H. Dvorachek, Jr., E. L. Braun, A. Errett, G. S. Saenz, M. D. Giles, M. Werner-Washburne, M. A. Nelson, and D. O. Natvig. 2001. Analysis of the pdx-1 (snz-1/sno-1) region of the Neurospora crassa genome: correlation of pyridoxine-requiring phenotypes with mutations in two structural genes. Genetics 157:1067-1075.
5. Belitsky, B. R. 2004. Physical and enzymological interaction of Bacillus subtilis proteins required for de novo pyridoxal 5′-phosphate biosynthesis. J. Bacteriol. 186:1191-1196.
6. Bilski, P., M. Y. Li, M. Ehrenshaft, M. E. Daub, and C. F. Chignell. 2000. Vitamin B6 (pyridoxine) and its derivatives are efficient singlet oxygen quenchers and potential fungal antioxidants. Photochem. Photobiol. 71:129-134.
7. Braun, E. L., E. K. Fuge, P. A. Padilla, and M. Werner-Washburne. 1996. A stationary-phase gene in Saccharomyces cerevisiae is a member of a novel, highly conserved gene family. J. Bacteriol. 178:6865-6872.
8. Del Sorbo, G., H. Schoonbeek, and M. A. De Waard. 2000. Fungal transporters involved in efflux of natural toxic compounds and fungicides. Fungal Genet. Biol. 30:1-15.
9. Dong, Y. X., S. Sueda, J. Nikawa, and H. Kondo. 2004. Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae. Eur. J. Biochem. 271:745-752.
10. Ehrenshaft, M., P. Bilski, M. Y. Li, C. F. Chignell, and M. E. Daub. 1999a. A highly conserved sequence is a novel gene involved in de novo vitamin B6 biosynthesis. Proc. Natl. Acad. Sci. USA 96:9374-9378.
11. Ehrenshaft, M., and M. E. Daub. 2001. Isolation of PDX2, a second novel gene in the pyridoxine biosynthesis pathway of eukaryotes, archaebacteria, and a subset of eubacteria. J. Bacteriol. 183:3383-3390.
12. Ehrenshaft, M., A. E. Jenns, K. R. Chung, and M. E. Daub. 1998. SOR1, a gene required for photosensitizer and singlet oxygen resistance in Cercospora fungi, is highly conserved in divergent organisms. Mol. Cell 1:603-609.
13. Gaber, R. F., M. C. Kielland-Brandt, and G. R. Fink. 1990. HOL1 mutations confer novel ion transport in Saccharomyces cerevisiae. Mol. Cell. Biol. 10: 643-652.
14. Goffeau, A., J. Park, I. T. Paulsen, J. L. Jonniaux, T. Dinh, P. Mordant, and M. H. Saier, Jr. 1997. Multidrug-resistant transport proteins in yeast: complete inventory and phylogenetic characterization of yeast open reading frames with the major facilitator superfamily. Yeast 13:43-54.
15. 14a.Gutz, H., H. Heslot, U. Leopold, and N. Loprieno. 1974. Schizosaccharomyces pombe, p. 395-446. In R. C. King (ed.), Handbook of genetics. Plenum Press, New York, N.Y.
16. Haworth, R. S., E. J. Cragoe, Jr., and L. Fliegel. 1993. Amiloride and 5-(N-ethyl-N-isopropyl)amiloride inhibit medium acidification and glucose metabolism by the fission yeast Schizosaccharomyces pombe. Biochim. Biophys. Acta 1145:266-272.
17. Heytler, P. G. 1979. Uncouplers of oxidative phosphorylation. Methods Enzymol. 55:462-472.
18. Hirose, K., R. Chumnantana, T. Nakashima, M. Ashiuchi, and T. Yagi. 2000. Efflux system for pyridoxine in Schizosaccharomyces pombe. Biosci. Biotechnol. Biochem. 64:2675-2679.
19. Jia, Z. P., N. McCullough, L. Wong, and P. G. Young. 1993. The amiloride resistance gene, carI, of Schizosaccharomyces pombe. Mol. Gen. Genet. 241:298-304.
20. John, R. A. 1995. Pyridoxal phosphate-dependent enzymes. Biochim. Biophys. Acta 1248:81-96.
21. Kleyman, T. R., and E. J. Cragoe, Jr. 1988. Amiloride and its analogs as tools in the study of ion transport. J. Membr. Biol. 105:1-21.
22. Lam, H. M., and M. E. Winkler. 1990. Metabolic relationships between pyridoxine (vitamin B6) and serine biosynthesis in Eschenchia coli K-12. J. Bacteriol. 172:6518-6528.
23. Liang, D. T., J. A. Hodson, and S. L. Forsburg. 1999. Reduced dosage of a single fission yeast MCM protein causes genetic instability and S phase delay. J. Cell Sci. 112:559-567.
24. Mitchison, J. M. 1970. Physiological and cytological methods for Schizosaccharomyces pombe. Methods Cell Physiol. 4:131-165.
25. Mittenhuber, G. 2001. Phylogenetic analyses and comparative genomics of vitamin B6 (pyridoxine) and pyridoxal phosphate biosynthesis pathways. J. Mol. Microbiol. Biotechnol. 3:1-20.
26. Moses, W., and M. A. Joslyn. 1953. The equivalence of thiamine and pyridoxine for a strain of Saccharomyces cerevisiae. J. Bacteriol. 66:197-203.
27. Nacken, V., T. Achstetter, and E. Degryse. 1996. Probing the limits of expression levels by varying promoter strength and plasmid copy number in Saccharomyces cerevisiae. Gene 175:253-260.
28. Nagy, M., F. Lacroute, and D. Thomas. 1992. Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts. Proc. Natl. Acad. Sci. USA 89:8966-8970.
29. Nelissen, B., R. De Wachter, and A. Goffeau. 1997. Classification of all putative permeases and other membrane plurispanners of the major facilitator superfamily encoded by the complete genome of Saccharomyces cerevisiae. FEMS Microbiol. Rev. 21:113-134.
30. Niederberger, C., H. Fankhauser, E. Edenharter, and M. E. Schweingruber. 1996. Amiloride toxicity in the fission yeast Schizosaccharomyces pombe is released by thiamine and mutations in the thiamine-reprissible gene car1. Gene 171:119-122.
31. Padilla, P. A., E. K. Fuge, M. E. Crawford, A. Errett, and M. Werner-Washburne. 1998. The highly conserved, coregulated SNO and SNZ gene families in Saccharomyces cerevisiae respond to nutrient limitation. J. Bacteriol. 180:5718-5726.
32. Paulsen, I. T., M. K. Sliwinski, B. Nelissen, A. Goffeau, and M. H. Saier, Jr. 1998. Unified inventory of established and putative transporters encoded within the complete genome of Saccharomyces cerevisiae. FEBS Lett. 430:116-125.
33. Rodriguez-Navarro, S., B. Llorente, M. T. Rodriguez-Manzaneque, A. Ramne, G. Uber, D. Marchesan, B. Dujon, E. Herrero, P. Sunnerhagen, and J. E. Perez-Ortin. 2002. Functional analysis of yeast gene families involved in metabolism of vitamins B1 and B6. Yeast 19:1261-1276.
34. Said, H. M., A. Ortiz, and T. Y. Ma. 2003. A carrier-mediated mechanism for pyridoxine uptake by human intestinal epithelial Caco-2 cells: regulation by a PKA-mediated pathway. Am. J. Physiol. Cell Physiol. 285:C1219-C1225.
35. Schulz, A. S., L. Atkin, and C. N. Frey. 1940. The biochemical classification of yeast strains. J. Bacteriol. 41:339-346.
36. Siam, R., W. P. Dolan, and S. L. Forsburg. 2004. Choosing and using Schizosaccharomyces pombe plasmids. Methods 33:189-198.
37. Stolz, J. 2003. Isolation and characterization of the plasma membrane biotin transporter from Schizosaccharomyces pombe. Yeast 20:223-231.
38. - mutants are caused by defects in pantothenate uptake. Eukaryot. Cell 3:406-412.
39. Stolz, J., and M. Vielreicher. 2003. Tpn1p, the plasma membrane vitamin B6 transporter of Saccharomyces cerevisiae. J. Biol. Chem. 278:18990-18996.
40. Tazuya, K., Y. Adachi, K. Masuda, K. Yamada, and H. Kumaoka. 1995. Origin of the nitrogen atom of pyridoxine in Saccharomyces cerevisiae. Biochim. Biophys. Acta 1244:113-116.
41. Thomas, B. J., and R. Rothstein. 1989. Elevated recombination rates in transcriptionally active DNA. Cell 56:619-630.
42. Van Huffel, C., E. Dubois, and F. Messenguy. 1994. Cloning and sequencing of Schizosaccharomyces pombe car1 gene encoding arginase. Expression of the arginine anabolic and catabolic genes in response to arginine and related metabolites. Yeast 10:923-933.
43. Wach, A., A. Brachat, R. Pohlmann, and P. Philippsen. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10:1793-1808.
44. White, R. L., and D. Spenser. 1996. Biosynthesis of thiamin, p. 680-686. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed. ASM Press, Washington, D.C.
45. Wightman, R., and P. A. Meacock. 2003. The THI5 gene family of Saccharomyces cerevisiae: distribution of homologues among the hemiascoinycetes and functional redundancy in the aerobic biosynthesis of thiamin from pyridoxine. Microbiology 149:1447-1460.
46. Wood, V., R. Gwilliam, M. A. Rajandream, M. Lyne, R. Lyne, A. Stewart, J. Sgouros, N. Peat, J. Hayles, S. Baker, D. Basham, S. Bowman, K. Brooks, D. Brown, S. Brown, T. Chillingworth, C. Churcher, M. Collins, R. Connor, A. Cronin, P. Davis, T. Feltwell, A. Fraser, S. Gentles, A. Goble, N. Hamlin, D. Harris, J. Hidalgo, G. Hodgson, S. Holroyd, T. Hornsby, S. Howarth, E. J. Huckle, S. Hunt, K. Jagels, K. James, L. Jones, M. Jones, S. Leather, S. McDonald, J. McLean, P. Mooney, S. Moule, K. Mungall, L. Murphy, D. Niblett, C. Odell, K. Oliver, S. O'Neil, D. Pearson, M. A. Quail, E. Rabbinowitsch, K. Rutherford, S. Rutter, D. Saunders, K. Seeger, S. Sharp, J. Skelton, M. Simmonds, R. Squares, S. Squares, K. Stevens, K. Taylor, R. G. Taylor, A. Tivey, S. Walsh, T. Warren, S. Whitehead, J. Woodward, G. Volckaert, R. Aert, J. Robben, B. Grymonprez, I. Weltjens, E. Vanstreels, M. Rieger, M. Schafer, S. Muller-Auer, C. Gabel, M. Fuchs, C. Fritze, E. Holzer, D. Moestl, H. Hubert, K. Borzym, I. Langer, A. Beck, H. Lehrach, R. Reinhardt, T. M. Pohl, P. Eger, W. Zimmermann, H. Wedler, R. Wambutt, B. Purnelle, A. Goffeau, E. Cadieu, S. Dreano, S. Gloux, V. Lelaure, et al. 2002. The genome sequence of Schizosaccharomyces pombe. Nature 415:871-830.
47. Wright, M. B., E. A. Howell, and R. F. Gaber. 1996. Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae. J. Bacteriol. 178:7197-7205.
48. Yagi, T., Y. Kim, Y. Hiraoka, A. Tanouchi, T. Yamamoto, and S. Yamamoto. 1996. Active transport activities of free B-6 vitamers in various yeast strains. Biosci. Biotechnol. Biochem. 60:893-897.
49. Yagi, T., A. Tanouchi, and Y. Hiraoka. 1998. Growth phase-dependent active transport of pyridoxine in a fission yeast, Schizosaccharomyces pombe. FEMS Microbiol. Lett. 161:145-150.
50. Zeidler, J., B. G. Sayer, and I. D. Spenser. 2003. Biosynthesis of vitamin B1 in yeast. Derivation of the pyrimidine unit from pyridoxine and histidine. Intermediacy of urocanic acid. J. Am. Chem. Soc. 125:13054-13105.
51. Zurlinden, A., and M. E. Schweingruber. 1997. Identification of a DNA element in the fission yeast Schizosaccharomyces pombe nmt1 (thi3) promoter involved in thiamine-regulated gene expression. J. Bacteriol 179:5956-5958.