Amino acid substitutions in membrane-spanning domains of Hol1, a member of the major facilitator superfamily of transporters, confer nonselective cation uptake in Saccharomyces cerevisiae

Journal of Bacteriology

Volumn 178, Issue 24, 1996, Pages 7197-7205

  Wright, Matthew B ^a,b   Howell, Elizabeth A ^a,c   Gaber, Richard F ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c CALVIN COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CALCIUM ION; CARRIER PROTEIN; CATION; DNA; HISTIDINE; HISTIDINOL; MEMBRANE PROTEIN; MESSENGER RNA; NUCLEOTIDE; PHENYLALANINE; POTASSIUM ION;

ALLELE; AMINO ACID SUBSTITUTION; ARTICLE; CATION TRANSPORT; CODON; DNA SEQUENCE; DRUG RESISTANCE; GENE DISRUPTION; IMMUNOBLOTTING; MEMBRANE; MUTANT; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION INITIATION;

SACCHAROMYCES CEREVISIAE;

EID: 0030447223     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.24.7197-7205.1996     Document Type: Article

References (37)

1. Ahmad, M., and H. Bussey. 1986. Yeast arginine permease: nucleotide sequence of the CAN1 gene. Curr. Genet. 10:587-592.
2. Anderson, J. A., S. S. Huprikar, L. V. Kochian, W. J. Lucas, and R. F. Gaber. 1992. Functional expression of a probable Arabidopsis thaliana potassium channel in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 89:3736-3740.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1993. Current protocols in molecular biology, vol. 1 and 2. John Wiley and Sons, New York.
4. Becker, D. M., and L. Guarente. 1991. High efficiency transformation of yeast by electroporation. Methods Enzymol. 194:182-187.
5. Bentley, J., L. S. Hyatt, K. Ainley, J. H. Parish, R. B. Herbert, and G. R. White. 1993. Cloning and sequence analysis of an Escherichia coli gene conferring bicyclomycin resistance. Gene 127:117-120.
6. + channel from Arabidopsis thaliana, and comparison with endogenous yeast channels and carriers. Proc. Natl. Acad. Sci. USA 92:2701-2705.
7. Cigan, A. M., and T. F. Donahue. 1987. Sequence and structural features associated with translational initiator regions in yeast - a review. Gene 59: 1-18.
8. Devereux, J., P. Haeberli, and O. Smithies. 1984. A comprehensive set of sequences and analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
9. Eisenberg, D. 1984. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. Ann. Rev. Biochem. 53:595-623.
10. Feinberg, A. P., and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
11. Fling, M. E., J. Kopf, A. Tamarkin, J. A. Gorman, H. A. Smit, and Y. Koltin. 1991. Analysis of a Candida albicans gene that encodes a novel mechanism for resistance to benomyl and methotrexate. Mol. Gen. Genet. 227:318-329,
12. Gaber, R. Unpublished results.
13. Gaber, R. F., M. C. Kielland-Brandt, and G. R. Fink. 1990. HOL1 mutations confer novel ion transport in Saccharomyces cerevisiae. Mol. Cell. Biol. 10: 543-652.
14. Gaber, R. F., C. A. Styles, and G. R. Fink. 1988. TRK1 encodes a plasma membrane protein required for high-affinity potassium transport in Saccharomyces cerevisiae. Mol. Cell. Biol. 8:2848-2859.
15. Goswitz, V. C., and R. J. Brooker. 1995. Structural features of the uniporter/ symporter/antiporter superfamily. Protein Science 4:534-537.
16. Griffith, J. K., M. E. Baker, D. A. Rouch, M. G. P. Page, R. A. Skurray, I. T. Paulsen, K. F. Chater, S. A. Baldwin, and P. J. F. Henderson. 1992. Membrane transport proteins: implications of sequence comparisons. Curr. Opin. Cell Biol. 4:684-695.
17. Hopp, T. P., and K. R. Woods. 1981. Prediction of protein antigenic determinants from amino acid sequences. Proc. Natl. Acad. Sci. USA 78:3824-3838.
18. Ito, H., Y. Fukuda, K. Murata. and A. Kimura. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153:163-168.
19. Jauniaux, J. C., and M. Grenson. 1990. GAP1, the general amino acid permease gene of Saccharomyces cerevisiae. Nucleotide sequence, protein similarity with the other bakers yeast amino acid permeases, and nitrogen catabolite repression. Eur. J. Biochem. 190:39-44.
20. Jia, A.-P., N. McCullough, L. Wong, and P. G. Young. 1993. The amiloride resistance gene, car1, of Schizosaccharomyces pombe. Mol. Gen. Genet. 241: 298-304.
21. + transporters in Saccharomyces cerevisiae. Mol. Cell. Biol. 11: 4266-4273.
22. Kolodziej, P., and R. Young. 1991. Epitope-tagging and protein surveillance. Methods Enzymol. 194:508-519.
23. Kyte, J., and R. F. Doolittle. 1982. A simple method of displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
24. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
25. Mueller, P. P., and A. G. Hinnebusch. 1986. Multiple upstream AUG codons mediate translational control of GCN4. Cell 45:201-207.
26. Nelissen, B., P. Mordant, J. L. Jonniaux, R. De Wachter, and A. Goffeau. 1995. Phylogenetic classification of the major superfamily of membrane transport facilitators, as deduced from yeast genome sequencing. FEBS Lett. 377:232-236.
27. Payne, G. M., E. N. Spudich, and G. F. Ames. 1985. A mutational hot-spot in the hisM gene of the histidine transport operon in Salmonella typhimurium is due to deletion of repeated sequences and results in an altered specificity of transport. Mol. Gen. Genet. 200:493-496.
28. Purnelle, B., J. Skala, L. Van Dyck, and A. Goffeau. 1992. The sequence of a 12-kb fragment on the left arm of yeast chromosome XI reveals five new open reading frames, including a zinc finger protein and a homolog of the UDP-glucose pyrophosphorylase from potato. Yeast 8:977-986.
29. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
30. Sasnauskas, K. 1992. Cloning and sequence analysis of a Candida maltosa gene which confers resistance to cycloheximide. Gene 116:105-108.
31. Schachtman, D. P., J. I. Schroeder, W. J. Lucas, J. A. Anderson, and R. F. Gaber. 1992. Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA. Science 258:1654-1658.
32. Sherman, F., G. R. Fink, and J. Hicks. 1986. Methods in yeast genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Sikorski, R. S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122:19-27.
34. Stirling, C. J., J. Rothblalt, M. Hosobuchi, R. Deshaies, and S. R. 1992. Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol. Biol. Cell. 3:129-142.
35. Tanaka, J.-I., and G. R. Fink. 1985. The histidine permease gene (HIP1) of Saccharomyces cerevisiae. Gene 38:205-214.
36. Trueheart, J., J. D. Boeke, and G. R. Fink. 1987. Two genes required for cell fusion during yeast conjugation: evidence for a pheromone-induced surface protein. Mol. Cell. Biol. 7:2316-2328.
37. Werner, M., F. Messenguy, and A. Pierard. 1987. The leader peptide of yeast gene CPA1 is essential for the translational repression of its expression. Cell 49:805-813.