Mutation of amino acids in the alpha 1,3-fucosyltransferase motif affects enzyme activity and Km for donor and acceptor substrates

Glycobiology

Volumn 15, Issue 2, 2005, Pages 165-175

  Jost, Franziska ^b   de Vries, Theodora ^a,b   Knegtel, Ronald M A ^c   Macher, Bruce A ^b  

^a UTRECHT UNIVERSITY   (Netherlands)

^b SAN FRANCISCO STATE UNIVERSITY   (United States)

^c VERTEX PHARMACEUTICALS   (United States)

Author keywords

Alanine scanning mutagenesis; Alpha 1,3 fucosyltransferase motif; Function; Molecular modeling; Structure

Indexed keywords

ALANINE; ALPHA 1,3 FUCOSYLTRANSFERASE; GUANOSINE DIPHOSPHATE FUCOSE; HELIX LOOP HELIX PROTEIN; MUTANT PROTEIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CARBOHYDRATE ANALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; GENE MUTATION; HUMAN; MUTAGENESIS; NUCLEOTIDE BINDING SITE; PREDICTION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN MOTIF; PROTEIN STRUCTURE; WESTERN BLOTTING; WILD TYPE;

AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; BINDING SITES; CONSERVED SEQUENCE; FUCOSYLTRANSFERASES; HUMANS; KINETICS; POINT MUTATION; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY;

EID: 13644260111     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/cwh157     Document Type: Article

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