Generation and functional in vivo characterization of a lipid kinase defective phosphatidylinositol 3-kinase Vps34p of Candida albicans

Microbiology

Volumn 151, Issue 1, 2005, Pages 81-89

  Günther, Juliane ^a   Nguyen, Monika ^a   Härtl, Albert ^a   Künkel, Waldemar ^b   Zipfel, Peter F ^a   Eck, Raimund ^a  

^a HANS KNÖLL INSTITUTE   (Germany)

^b UNIVERSITY OF APPLIED SCIENCES JENA   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHIMERIC PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; TARGET OF RAPAMYCIN KINASE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; AUTOPHOSPHORYLATION; BINDING SITE; CANDIDA ALBICANS; CANDIDIASIS; CELL VACUOLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DEFECT; ENZYME PHOSPHORYLATION; ESCHERICHIA COLI; FUNGAL GENE; FUNGAL STRAIN; FUNGAL VIRULENCE; FUNGUS GROWTH; FUNGUS HYPHAE; FUNGUS MUTANT; GENE INSERTION; HUMAN; HUMAN CELL; IN VIVO STUDY; MALE; MOUSE; MOUTH MUCOSA; NONHUMAN; OSMOTIC STRESS; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; STRAIN DIFFERENCE; TEMPERATURE DEPENDENCE; TRANSPORT VESICLE; WILD TYPE;

1-PHOSPHATIDYLINOSITOL 3-KINASE; AMINO ACID SEQUENCE; ANIMALS; ANIMALS, OUTBRED STRAINS; BIOLOGICAL TRANSPORT; CANDIDA ALBICANS; CANDIDIASIS; ESCHERICHIA COLI; GENE DELETION; MALE; MICE; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); RECOMBINANT PROTEINS; VACUOLES; VIRULENCE;

CANDIDA; CANDIDA ALBICANS; ESCHERICHIA COLI; TOR;

EID: 13444261420     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.27333-0     Document Type: Article

References (29)

1. Augsten, M., Hübner, C., Nguyen, M., Künkel, W., Härtl, A. & Eck, R. (2002). Defective hyphal induction of a Candida albicans phosphatidylinositol 3-phosphate 5-kinase null mutant on solid media does not lead to decreased virulence. Infect Immun 70, 4462-4470.
2. Bailey, A., Wadsworth, E. & Calderone, R. (1995). Adherence of Candida albicans to human buccal epithelial cells: host-induced protein synthesis and signaling events. Infect Immun 63, 569-572.
3. Bondev, A., Rubio, I. & Wetzker, R. (1999). Differential regulation of lipid and protein kinase activities of phosphoinositide 3-kinase gamma in vitro. Biol Chem 380, 1337-1340.
4. Bondeva, T., Pirola, L., Bulgarelli-Leva, G., Rubio, I., Wetzker, R. & Wymann, M. P. (1998). Bifurcation of lipid and protein kinase signals of PI3Kgamma to the protein kinases PKB and MAPK. Science 282, 293-296.
5. Bruckmann, A., Künkel, W., Härtl, A., Wetzker, R. & Eck, R. (2000). A phosphatidylinositol 3-kinase of Candida albicans influences adhesion, filamentous growth, and virulence. Microbiology 146, 2755-2764.
6. Bruckmann, A., Künkel, W., Augsten, K., Wetzker, R. & Eck, R. (2001). The deletion of CaVPS34 in the human pathogenic yeast Candida albicans causes defects in vesicle-mediated protein sorting and nuclear segregation. Yeast 18, 343-353.
7. Burda, P., Padilla, S. M., Sarkar, S. & Emr, S. D. (2002). Retromer function in endosome-to-Golgi retrograde transport is regulated by the yeast Vps34 PtdIns 3-kinase. J Cell Sci 115, 3889-3900.
8. Cutler, J. E. (1991). Putative virulence factors of Candida albicans. Annu Rev Microbiol 45, 187-218.
9. Czupalla, C., Culo, M., Muller, E. C., Brock, C., Reusch, H. P., Spicher, B., Krause, E. & Nürnberg, B. (2003). Identification and characterization of the autophosphorylation sites of phosphoinositide 3-kinase isoforms beta and gamma. J Biol Chem 278, 11536-11545.
10. DeBacker, M. D., Maes, D., Vandoninck, S., Logghe, M., Contreras, R. & Luyten, W. H. (1999). Transformation of Candida albicans by electroporation. Yeast 15, 1609-1618.
11. DeCamilli, P., Emr, S. D., McPherson, P. S. & Novick, P. (1996). Phosphoinositides as regulators in membrane traffic. Science 271, 1533-1539.
12. Eck, R., Bruckmann, A., Wetzker, R. & Künkel, W. (2000). A phosphatidylinositol 3-kinase of Candida albicans: molecular cloning and characterization. Yeast 16, 933-944.
13. Fonzi, W. A. & Irwin, M. Y. (1993). Isogenic strain construction and gene mapping in Candida albicans. Genetics 134 717-728.
14. Herman, P. K. & Emr, S. D. (1990). Characterization of VPS34, a gene required for vacuolar protein sorting and vacuole segregation in Saccharomyces cerevisiae. Mol Cell Biol 10, 6742-6754.
15. Kihara, A., Noda, T., Ishihara, N. & Ohsumi, Y. (2001). Two distinct Vps34 phosphatidylinositol 3-kinase complexes function in autophagy and Carboxypeptidase Y sorting in Saccharomyces cerevisiae. J Cell Biol 152, 519-530.
16. Köhler, J. R. & Fink, G. R. (1996). Candida albicans strains heterozygous and homozygous for mutations in mitogen-activated protein kinase signaling components have defects in hyphal development. Proc Natl Acad Sci U S A 93, 13223-13228.
17. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680-685.
18. Munn, A. L. & Riezman, H. (1994). Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. J Cell Biol 127, 373-386.
19. Odds, F. C. (1994). Pathogenesis of Candida infections. J Am Acad Dermatol 31, S2-S5.
20. Schu, P. V., Takegawa, K., Fry, M. J., Stack, J. H., Waterfield, M. D. & Emr, S. D. (1993). Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting. Science 260, 88-91.
21. Stack, J. H. & Emr, S. D. (1994). Vps34p required for yeast vacuolar protein sorting is a multiple specificity kinase that exhibits both protein kinase and phosphatidylinositol-specific PI 3-kinase activities. J Biol Chem 269, 31552-31562.
22. Stoyanov, B., Volinia, S., Hanck, T. & 7 other authors (1995). Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase. Science 269, 690-693.
23. Toker, A. & Cantley, L. C. (1997). Signalling through the lipid products of phosphoinositide-3-OH kinase. Nature 387 673-676.
24. Vanhaesebroeck, B., Leevers, S. J., Panayotou, G. & Waterfield, M. D. (1997). Phosphoinositide 3-kinases: a conserved family of signal transducers. Trends Biochem Sci 22, 267-272.
25. Vida, T. A. & Emr, S. D. (1995). A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J Cell Biol 128, 779-792.
26. Walsh, J. P., Caldwell, K. K. & Majerus, P. W. (1991). Formation of phosphatidylinositol 3-phosphate by isomerization from phosphatidylinositol 4-phosphate. Proc Natl Acad Sci U S A 88 9184-9187.
27. Wurmser, A. E. & Emr, S. D. (1998). Phosphoinositide signaling and turnover: PtdIns(3)P, a regulator of membrane traffic, is transported to the vacuole and degraded by a process that requires lumenal vacuolar hydrolase activities. EMBO J 17, 4930-4942.
28. Wymann, M. P., Bulgarelli-Leva, G., Zvelebil, M. J., Pirola, L., Vanhaesebroeck, B., Waterfield, M. D. & Panayotou, G. (1996). Wortmannin inactivates phosphoinositide 3-kinase by covalent modification of Lys-802, a residue involved in the phosphate transfer reaction. Mol Cell Biol 16, 1722-1733.
29. Zhou, K., Takegawa, K., Emr, S. D. & Firtel, R. A. (1995). A phospatidylinositol (PI) kinase gene family in Dictyostelium discoideum: biological roles of putative mammalian p110 and yeast Vps34p PI 3-kinase homologs during growth and development. Mol Cell Biol 15, 5645-5656.