Functional domain organization of the potato α-glucan, water dikinase (GWD): Evidence for separate site catalysis as revealed by limited proteolysis and deletion mutants

Biochemical Journal

Volumn 385, Issue 2, 2005, Pages 355-361

  Mikkelsen, René ^a   Blennow, Andreas ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Dikinase; Domain structure; Limited proteolysis; Phosphorylation; Starch

Indexed keywords

ADENOSINETRIPHOSPHATE; CATALYSIS; ENZYME KINETICS; REACTION KINETICS;

AMYLOPECTIN; GLUCOSYL RESIDUE; PHOSPHORYL GROUP TRANSFER; PHOSPHORYLATION;

STARCH;

1,4 ALPHA GLUCAN BRANCHING ENZYME; ADENOSINE TRIPHOSPHATE; ALPHA GLUCAN WATER DIKINASE; HISTIDINE; PHOSPHATE; PYRUVATE PHOSPHATE DIKINASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; AUTOPHOSPHORYLATION; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; DELETION MUTANT; DNA SEQUENCE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME MECHANISM; ENZYME STRUCTURE; NONHUMAN; PHOSPHORYLATION; POTATO; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FUNCTION;

ALTERNATIVE SPLICING; BINDING SITES; CATALYTIC DOMAIN; CIRCULAR DICHROISM; ENDOPEPTIDASE K; HYDROLYSIS; MUTATION; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PHOSPHOTRANSFERASES (PAIRED ACCEPTORS); PROTEIN STRUCTURE, TERTIARY; PROTEINS; SOLANUM TUBEROSUM;

BACTERIA (MICROORGANISMS); SOLANUM TUBEROSUM; TUBEROSUM;

EID: 12844268556     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20041119     Document Type: Article

References (28)

1. Bay-Smidt, A. M., Wischmann, B., Olsen, C. E. and Nielsen, T. H. (1994) Starch bound phosphate in potato as studied by a simple method for determination of organic phosphate and P-31-Nmr. Starch Staerke. 46, 167-172
2. Hizukuri, S., Tabata, S. and Nikuni, Z. (1970) Studies on starch phosphate, Part 1. Estimation of glucose-6-phosphate residues in starch and the presence of other bound phosphate(s). Starch Staerke. 22, 338-343
3. Blennow, A., Engelsen, S. B., Munck, L. and Møller, B. L. (2000) Starch molecular structure and phosphorylation investigated by a combined chromatographic and chemometric approach. Carbohydr. Polym. 41, 163-174
4. Tabata, S., Nagata, K. and Hizukuri, S. (1975) Studies on starch phosphates, Part 3. On the esterified phosphates in some cereal starches. Starch Staerke. 27, 333-335
5. Yu, T.-S., Kofler, H., Häusler, R. E., Hille, D., Flügge, H.-I., Zeeman, S. C., Smith, A. M., Kossmann, J., Lloyd, J., Ritte, G. et al. (2001) The Arabidopsis sex1 mutant is defective in the R1 protein, a general regulator of starch degradation in plants, and not in the chloroplast hexose transporter. Plant Cell 13, 1907-1918
6. Nielsen, T. H., Wischmann, B., Enevoldsen, K. and Møller, B. L. (1994) Starch phosphorylation in potato tubers proceeds concurrently with de novo biosynthesis of starch. Plant Physiol. 105, 111-117
7. Wischmann, B., Nielsen, T. H. and Møller, B. L. (1999) In vivo biosynthesis of phosphorylated starch in intact potato amyloplasts. Plant Physiol. 119, 455-462
8. Viksø-Nielsen, A., Blennow, A., Jørgensen, K., Kristensen, K. H., Jensen, A. and Møller, B. L. (2001) Structural, physicochemical, and pasting properties of starches from potato plants with repressed r1-gene. Biomacromolecules 2, 836-843
9. Lorberth, R., Ritte, G., Willmitzer, L. and Kossmann, J. (1998) Inhibition of a starch-granule-bound protein leads to modified starch and repression of cold sweetening. Nat. Biotechnol. 16, 473-477
10. Blennow, A., Engelsen, S. B., Nielsen, T. H., Baunsgaard, L. and Mikkelsen, R. (2002) Starch phosphorylation: a new front line in starch research. Trends Plant Sci. 7, 445-450
11. Ritte, G., Eckermann, N., Haebel, S., Lorberth, R. and Steup, M. (2000) Compartmentation of the starch-related R1 protein in higher plants. Starch Starke. 52, 145-149
12. Ritte, G., Lloyd, J. R., Eckermann, N., Rottmann, A., Kossmann, J. and Steup, M. (2002) The starch-related R1 protein is an alpha-glucan, water dikinase. Proc. Natl. Acad. Sci. U.S.A. 99, 7166-7171
13. Mikkelsen, R., Baunsgaard, L. and Blennow, A. (2004) Functional characterization of alpha-glucan, water dikinase, the starch phosphorylating enzyme. Biochem. J. 377, 525-532
14. Herzberg, O., Chen, C. C. H., Kapadia, G., McGuire, M., Carroll, L. J., Noh, S. J. and Dunaway-Mariano, D. (1996) Swiveling-domain mechanism for enzymatic phosphotransfer between remote reaction sites. Proc. Natl. Acad. Sci. U.S.A. 93, 2652-2657
15. Herzberg, O., Chen, C. C. H., Liu, S., Tempczyk, A., Howard, A., Wei, M., Ye, D. M. and Dunaway-Mariano, D. (2002) Pyruvate site of pyruvate phosphate dikinase: crystal structure of the enzyme-phosphonopyruvate complex, and mutant analysis. Biochemistry 41, 780-787
16. Bradford, M. M. (1976) Rapid and sensitive method for quantitation of microgram quantities of protein utilizing principle of protein-dye binding. Anal. Biochem. 72, 248-254
17. Smith, P. K., Krohn, R. I., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J. and Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76-85
18. Laemmli, U. K. (1970) Cleavage of structural proteins during assembly of head of bacteriophage-T4. Nature (London) 227, 680-685
19. Burnette, W. N. (1981) Western blotting - electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated Protein-A. Anal. Biochem. 112, 195-203
20. Andrade, M. A., Chacón, P., Merelo, J. J. and Morán, F. (1993) Evaluation of secondary structure of proteins from UV circular dichroism using an unsupervised learning neural network. Protein Eng. 6, 383-390
21. Fonda, M. L. (1976) Bromopyruvate inactivation of glutamate apodecarboxylase - kinetics and specificity. J. Biol. Chem. 251, 229-235
22. Fontana, A., Fassina, G., Vita, C., Dalzoppo, D., Zamai, M. and Zambonin, M. (1986) Correlation between sites of limited proteolysis and segmental mobility in thermolysin. Biochemistry 25, 1847-1851
23. Johnson, W. C. (1988) Secondary structure of proteins through circular-dichroism spectroscopy. Annu. Rev. Biophys. Biophys. Chem. 17, 145-166
24. Williams, R. W. (1986) Protein secondary structure-analysis using Raman amide-I and amide-III spectra. Methods Enzymol. 130, 311-331
25. Lusetti, S. L., Shaw, J. J. and Cox, M. M. (2003) Magnesium ion-dependent activation of the RecA protein involves the C terminus. J. Biol. Chem. 278, 16381-16388
26. Ason, B., Bertram, J. G., Hingorani, M. M., Beechem, J. M., O'Donnell, M., Goodman, M. F. and Bloom, L. B. (2000) A model for Escherichia coli DNA polymerase III holoenzyme assembly at primer/template ends - DNA triggers a change in binding specificity of the gamma complex clamp loader. J. Biol. Chem. 275, 3006-3015
27. Reimann, R., Hippler, M., Machelett, B. and Appenroth, K. J. (2004) Light induces phosphorylation of glucan water dikinase, which precedes starch degradation in turions of the duckweed Spirodela polyrhiza. Plant Physiol. 135, 121-128
28. Wei, M., Li, Z., Ye, D. M., Herzberg, O. and Dunaway-Mariano, D. (2000) Identification of domain-domain docking sites within Clostridium symbiosum pyruvate phosphate dikinase by amino acid replacement. J. Biol. Chem. 275, 41156-41165