Exogenous nucleosides modulate the expression of rat liver extracellular matrix genes in single cultures of primary hepatocytes and a liver stellate cell line and in their co-culture

Clinical Nutrition

Volumn 23, Issue 1, 2004, Pages 43-51

  Arnaud, Alexandra ^a   Fontana, Luis ^b   Sáez Lara, María José ^b   Gil, Ángel ^b   López Pedrosa, José María ^a  

^a ABBOTT LABORATORIES   (Spain)

^b UNIVERSITY OF GRANADA   (Spain)

Author keywords

Co cultures; Extracellular matrix; Hepatocytes; Liver stellate cells; Nucleosides

Indexed keywords

BIOLOGICAL MARKER; COLLAGEN; COLLAGENASE 3; FIBRONECTIN; LAMININ; NUCLEOSIDE; PROCOLLAGEN; SCLEROPROTEIN;

ANIMAL CELL; ARTICLE; CELL FUNCTION; CELL INTERACTION; CELL LINE; CELL TYPE; COCULTURE; COLLAGEN SYNTHESIS; CONTROLLED STUDY; EXTRACELLULAR MATRIX; GENE EXPRESSION; LIVER CELL; LIVER CELL CULTURE; LIVER FUNCTION; MODULATION; MOLECULAR MODEL; NONHUMAN; PROTEIN CONTENT; PROTEIN SECRETION; RAT; STELLATE CELL;

EID: 1242342230     PISSN: 02615614     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0261-5614(03)00087-6     Document Type: Article

References (47)

1. Brecher AS, Hinko A, Twining SS. A proposed sequence of reactions leading to collagen biosynthesis, fibrosis and hypertension in alcoholics. J Invest Med 1996;44:36-41.
2. Mato JM, Cámara J, Fernández de Paz J, et al. S-adenosylmethionine in alcoholic liver cirrhosis: a randomized, placebo-controlled, doubled-blind, multicenter clinical trial. J Hepatol 1999;30:1081-9.
3. Li J, Kim C, Leo MA, Mak KM, Rojkind M, Lieber CS. Polyunsaturated lecithin prevents acetaldehyde-mediated hepatic collagen accumulation by stimulating collagenase activity in cultured lipocytes. Hepatology 1992;15:373-81.
4. Sánchez-Pozo A, Rueda R, Fontana L, Gil A. Dietary nucleotides and cell growth. Trends Comp Biochem Physiol 1998;5:99-111.
5. Cosgrove M. Nucleotides. Nutrition 1998;14:748-51.
6. Grimble GK, Westwood DM. Nucleotides as immunomodulators in clinical nutrition. Curr Opin Nutr Med Care 2001;4:57-64.
7. Torres-López MI, Fernández MI, Fontana L, Gil A, Ríos A. Influence of dietary nucleotides on liver structural recovery and hepatocyte binuclearity in cirrhosis induced by thioacetamide. Gut 1996;38:260-4.
8. Torres MI, Fernández MI, Gil A, Ríos A. Effect of dietary nucleotides on degree of fibrosis and steatosis induced by oral intake of thioacetamide. Digest Dis Sci 1997;42:1322-8.
9. Fontana L, Moreira E, Torres MI, Fernández MI, Ríos A, Sánchez-Medina F, Gil A. Dietary nucleotides correct plasma and liver microsomal fatty acid alterations in rats with liver cirrhosis induced by oral intake of thioacetamide. J Hepatol 1998;28:662-9.
10. Hernández-Muñoz R, Díaz-Mun̂oz M, Suárez-Cuenca JA, et al. Adenosine reverses a preestablished CCl4-induced micronodular cirrosis through enhancing collagenolytic activity and stimulating hepatocyte cell proliferation in rats. Hepatology 2001;34:677-87.
11. Pérez MJ, Suárez A, Gómez-Capilla JA, Sánchez de Medina F, Gil A. Dietary-nucleotides supplementation reduces thioacetamide-induced liver fibrosis in rats. J Nutr 2002;132:652-7.
12. +-nucleoside cotransporter proteins (hCNT3 and mCNT3) broadly selective for purine and pyrimidine nucleosides (system cib). J Biol Chem 2001;276:2914-27.
13. Anderson CM, Parkinson FE. Potential signalling roles for UTP and UDP: sources, regulation and release of uracil nucleotides. Trends Pharm Sci 1997;18:387-92.
14. Moyer JD, Oliver JT, Handschumacher RE. Salvage of circulating pyrimidine nucleosides in the rat. Cancer Res 1981;41:3010-7.
15. 14C]deoxycytidine in cultured L1210 cells. Cancer Lett 1983;19:147-57.
16. Seglen PO. Preparation of isolated rat liver cells. In: Prescott DM, editor. Methods in Cell Biology, Vol. XIII. New York: Academic Press; 1976. p. 29-83.
17. Reid LM, Jefferson DM. Culturing hepatocytes and other differentiated cells. Hepatology 1984;4:548-59.
18. 4-cirrhotic livers. Differences in the production of interleukin-6. Lab Invest 1991;65:644-53.
19. 4-cirrhotic rat are heterogeneous with regard to proliferation, extracellular matrix componentes, interleukin-6 and connexin 43. Lab Invest 1993;69:210-6.
20. Rojkind M, Novikoff P M, Greenwel P, et al. Characterization and functional studies on rat liver fat-storing cell line and freshly isolated hepatocyte coculture system. Am J Pathol 1995;146:1508-20.
21. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156-9.
22. Schwarzbauer J, Tamkum J, Lemischka I, Hynes R. Three different fibronectin mRNAs arise by alternative splicing within the coding region. Cell 1983;35:421-31.
23. Sasaki M, Kato S, Hohno K, Martin GR, Yamada Y. Sequence of the cDNA encoding the laminin B1 chain reveals a multidomain protein containing cysteine-rich repeats. Proc Natl Acad Sci USA 1987;84:935-9.
24. Genovese C, Rowe D, Kream B. Construction of DNA sequences complementary to rat α1 and α2 collagen mRNA and their use in studyinf the regulation of type I collagen synthesis by 1,25-dihydroxyvitamin D. Biochemistry 1984;23:6210-6.
25. Vuorio E, Makela J, Vuorio T, Poole A, Wagner J. Characterization of excessive collagen production during development of pulmonary fibrosis induced by chronic silica inhalation in rats. Br J Exp Pathol 1989;70:305-15.
26. Henriet P, Rousseau GG, Eeckhout Y. Cloning and sequencing of mouse collagenase cDNA. Divergence of mouse and rat collagenases from other mammalian collagenases. FEBS Lett 1992;310:175-8.
27. Coloumbe B, Skup D. In vitro synthesis of the active tissue inhibitor of metalloproteinases encoded by a complementary DNA from virus-infected murine fibroblasts. J Biol Chem 1988;263:1439-43.
28. Zern MA, Chakraborty PR, Ruiz-Opazo N, Yap SH, Shafritz DA. Development and use of a rat albumin cDNA clone to evaluate the effect of chronic ethanol administration on hepatic protein synthesis. Hepatology 1983;3:317-22.
29. Bradford MA. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
30. Peterkofsky P, Haralson MA, DiMari SJ, Miller EJ. Types I, III and V collagen and total collagen from cultured cells and tissues. In: Haralson MA, Hassell JR, editors. Extracellular matrix: a practical approach. Oxford: Oxford University Press; 1995. p. 32-6.
31. 4-induced cirrhosis. Lab Invest 1985;53:166-86.
32. Clément B, Loréal O, Rescan PY. Cellular origin of the hepatic extracellular matrix. In: Gressner AM, Ramadori G, editors. Molecular and cellular biology of liver fibrogenesis. Lancaster: Kluwer; 1992. p. 85 98.
33. Berman B, Duncam MR. Pentoxifyline inhibits normal human dermal fibroblast in vitro proliferation, collagen, glycosaminoglycan, and fibronectin production, and increases collagenase activity. J Invest Dermatol 1989;92: 605-10.
34. Windmeier C, Gressner, AM . Effect of pentoxifylline on the fibrogenic functions of cultured rat liver fat-storing cells and myofibroblasts. Biochem Pharmacol 1996;51:577-84.
35. Arnaud A, Fontana L, Angulo AJ, Gil A, López-Pedrosa JM. Exogenous nucleosides alter the intracellular nucleotide pool in hepatic cell cultures. Implications in cell proliferation and function. Clin Nutr 2003, this volume doi:10.1016/S0261-5614(03)00037-2
36. Rijcken WR, Overdijk B, Van Den Eijnden DH, Ferwerda W. The effect of increasing nucleotide-sugar concentrations on the incorporation of sugars into glycoconjugated in rat hepatocytes. Biochem J 1995;305:865-70.
37. Valdes R, Ortega MA, Casado FJ, Felipe A, Gil A, Sánchez-Pozo A, Pastor-Anglada M. Nutritional regulation of nucleoside transporter expresión in rat small intestine. Gastroenterology 2000;119:1623-30.
38. Theret N, Musso O, L'Helgoualc'h A, Clement B. Activation of matrix metalloproteinase-2 from hepatic stellate cells requires interactions with hepatocytes. Am J Pathol 1997;150:51-8.
39. Koda H, Okumo M, Imai S, Moriwaki H, Muto Y, Kawada N, Kojima S. Retinoic acid-stimulated liver stellate cells supress the production of albumin from parenchymal cells. Biochem Biophys Res Commun 1996;221:565-9.
40. Gressner AM, Polzar B, Lahme B, Mannherz HG. Induction of rat liver parenchymal cell apoptosis by hepatic myofibroblast via transforming growth factor beta. Hepatology 1996;23:571-81.
41. Kawada N, Kuroki T, Uoya M, Kaneda K. Inhibition of myofibroblastic transformation of cultured rat hepatic stellate cells by methylxantines and dibutyril cAMP. Digest Dis Sci 1996;5:1022-9.
42. Liu F, Thompson MA, Wagner S, Green MR. Activating transcription factor-1 can mediate Ca + and cAMP inducible transcriptional activation. J Biol Chem 1993;268:6714-20.
43. Edgecombe M, Alexander G, McLennan, Fisher MJ. Diadenosine polyphosphate and the control of cyclic AMP concentrations in isolated rat liver cells. FEBS Lett 1999;457:455 8.
44. Houglum K, Kwan S, Chojkier M. Proliferation of hepatic stellate cells is inhibited by phosphosrylation of CREB on serine 133. J Clin Invest 1997;99:1322-8.
45. Bissell DM, Guzelian PS. Ascorbic acid deficiency and cytochrome P-450 in adult rat hepatocytes in primary monolayer culture. Arch Biochem Biophys 1979;192:569-76.
46. Sirica AE, Richards W, Tsukada I, Sattler CA, Pitot HC. Fetal phenotypic expression by adult rat hepatocytes on collagen ge/nylon meshes. Proc Natl Acad Sci USA 1979;76:283-7.
47. Tanaka K, Sato M, Tomita Y, Ichihara A. Biochemical studies on liver functions in primary cultured hepatocytes of adult rats. Hormonal effects on cell viability and protein synthesis. J Biochem 1978;84:937-46.