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In pulling the protected UPy control polymer B, we most time observed only a single peak. At about 0.1% rate, we observed two or more peaks on one curve. For those showing multiple peaks, they could not be fitted with the wormlike chain (WLC) model for a single chain, indicating that the multiple peaks originated from multiple chain adsorptions.
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note
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In pulling the deprotected UPy modular polymer A, the success rate of picking up a chain is about 2%. In one set of experiments with a total of 48 force-extension curves, the statistical information is as follows: 6 curves show two peaks, 16 curves show three peaks, 16 curves show four peaks, 7 curves show five peaks, 2 curves show six peaks, and 1 curve shows seven peaks. Among all the unfolding peaks observed from this pool of curves, 52 peaks show peak force around 100 pN and 61 peaks show peak force around 200 pN. Of this pool of curves, about 70% of the final extension is in the range of 200-300 nm.
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