Role of Active-Site Residues Thr81, Ser82, Thr85, Gln157, and Tyr158 in Yeast Cystathionine β-Synthase Catalysis and Reaction Specificity

Biochemistry

Volumn 43, Issue 7, 2004, Pages 1963-1971

  Aitken, Susan M ^a   Kirsch, Jack F ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CONDENSATION; CONFORMATIONS; ENZYME INHIBITION; HYDROLYSIS; MUTAGENESIS; YEAST;

MUTANTS; REACTION SPECIFICITY;

ENZYMES;

ACRYLIC ACID DERIVATIVE; AMINOACRYLATE; AMMONIA; CYSTATHIONINE; CYSTATHIONINE BETA SYNTHASE; GLUTAMINE; HOMOCYSTEINE; SERINE; THREONINE; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; HYDROLYSIS; MUTANT; MUTATION; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; WILD TYPE; YEAST;

ALANINE; AMINO ACID SUBSTITUTION; BINDING SITES; CATALYSIS; CHROMATOGRAPHY, AFFINITY; CYSTATHIONINE BETA-SYNTHASE; GLUTAMINE; KINETICS; MODELS, CHEMICAL; MUTAGENESIS, SITE-DIRECTED; NICKEL; PHENYLALANINE; PYRUVIC ACID; SACCHAROMYCES CEREVISIAE PROTEINS; SERINE; SUBSTRATE SPECIFICITY; THREONINE; TYROSINE;

EID: 1242285467     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi035496m     Document Type: Article

References (32)

1. Carson, N. A. J., Dent, C. E., Field, C. M. B., Neill, D. W., Westall, R. G., and Cusworth, D. C. (1963) Arch. Dis. Child. 38, 425-436.
2. Mudd, S. H., Laster, L., Finkelstein, J. D., and Irreverre, F. (1964) Science 143, 1443-1445.
3. Mudd, S. H., Levy, H. L., and Skovby, F. (1989) in The Metabolic Basis of Inherited Disease (Scriver, C. R., Beaudet, A. L., Sly, W. S., and Valle, D., Eds.) pp 693-734, McGraw-Hill, New York.
4. Kraus, J. P., Janosik, M., Kozich, V., Mandell, R., Shih, V., Sperandeo, M. P., Sebastio, G., de Franchis, R., Andria, G., Kluijtmans, L. A., Blom, H., Boers, G. H., Gordon, R. B., Kamoun, P., Tsai, M. Y., Kruger, W. D., Koch, H. G., Ohura, T., and Gaustadnes, M. (1999) Hum. Mutat. 13, 362-375.
5. Kery, V., Bukovska, G., and Kraus, J. P. (1994) J. Biol. Chem. 269, 25283-25288.
6. Taoka, S., Ohja, S., Shan, X., Kruger, W. D., and Banerjee, R. (1998) J. Biol. Chem. 273, 25179-25184.
7. Taoka, S., Lepore, B. W., Kabil, O., Ojha, S., Ringe, D., and Banerjee, R. (2002) Biochemistry 41, 10454-10461.
8. Meier, M., Janosik, M., Kery, V., Kraus, J. P., and Burkhard, P. (2001) Embo J. 20, 3910-3916.
9. Maclean, K. N., Janosik, M., Oliveriusova, J., Kery, V., and Kraus, J. P. (2000) J. Inorg. Biochem. 81, 161-171.
10. Jhee, K. H., McPhie, P., and Miles, E. W. (2000) J. Biol. Chem. 275, 11541-11544.
11. Jhee, K. H., Niks, D., McPhie, P., Dunn, M. F., and Miles, E. W. (2001) Biochemistry 40, 10873-10880.
12. Taoka, S., and Banerjee, R. (2002) J. Biol. Chem. 277, 22421-22425.
13. Aitken, S. M., and Kirsch, J. F. (2003) Biochemistry 42, 571-578.
14. Miles, E. W. (2001) Chem. Rec. 1, 140-151.
15. Tai, C. H., and Cook, P. F. (2001) Acc. Chem. Res. 34, 49-59.
16. Rege, V. D., Kredich, N. M., Tai, C. H., Karsten, W. E., Schnackerz, K. D., and Cook, P. F. (1996) Biochemistry 35, 13485-13493.
17. Burkhard, P., Tai, C. H., Ristroph, C. M., Cook, P. F., and Jansonius, J. N. (1999) J. Mol. Biol. 291, 941-953.
18. Kashiwamata, S., and Greenberg, D. M. (1970) Biochim. Biophys. Acta 212, 488-500.
19. Higuchi, R. (1990) in PCR Protocols: A Guide to Methods and Applications (Gelfand, M. A., Sninsky, D. H., and White, J. J., Eds.) pp 177-183, Academic Press, New York.
20. Jhee, K. H., McPhie, P., and Miles, E. W. (2000) Biochemistry 39, 10548-10556.
21. Likos, J. J., Ueno, H., Feldhaus, R. W., and Metzler, D. E. (1982) Biochemistry 21, 4377-4386.
22. Ueno, H., Likos, J. J., and Metzler, D. E. (1982) Biochemistry 21, 4387-4393.
23. Ahmed, S. A., Ruvinov, S. B., Kayastha, A. M., and Miles, E. W. (1991) J. Biol. Chem. 266, 21548-21557.
24. Christen, P., and Mehta, P. K. (2001) Chem. Rec. 1, 436-447.
25. Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., Gehring, H., and Christen, P. (1984) J. Mol. Biol. 174, 497-525.
26. Capitani, G., McCarthy, D. L., Gut, H., Grutter, M. G., and Kirsch, J. F. (2002) J. Biol. Chem. 277, 47935-47942.
27. Clausen, T., Huber, R., Laber, B., Pohlenz, H. D., and Messerschmidt, A. (1996) J. Mol. Biol. 262, 202-224.
28. Clausen, T., Huber, R., Prade, L., Wahl, M. C., and Messerschmidt, A. (1998) EMBO J. 17, 6827-6838.
29. Rhee, S., Parris, K. D., Hyde, C. C., Ahmed, S. A., Miles, E. W., and Davies, D. R. (1997) Biochemistry 36, 7664-7680.
30. Schirch, V., Shostak, K., Zamora, M., and Guatam-Basak, M. (1991) J. Biol. Chem. 266, 759-764.
31. Cook, P. F., Hara, S., Nalabolu, S., and Schnackerz, K. D. (1992) Biochemistry 31, 2298-2303.
32. Burkhard, P., Rao, G. S., Hohenester, E., Schnackerz, K. D., Cook, P. F., and Jansonius, J. N. (1998) J. Mol. Biol. 283, 121-133.