Bacterial expression, purification and functional characterization of a recombinant chimeric Fab derived from murine mAb BCF2 that neutralizes the venom of the scorpion Centruroides noxius hoffmann

Toxicon

Volumn 43, Issue 1, 2004, Pages 43-51

  Selisko, Barbara ^a,b   Cosío, Gabriela ^a   García, Consuelo ^a   Becerril, Baltazar ^a   Possani, Lourival D ^a   Horjales, Eduardo ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

^b Centre National de la Recherche Scientifique   (France)

Author keywords

Antibody expression; Centruroides noxius; Chimeric Fab; Neutralization; Scorpion; Toxin

Indexed keywords

AMMONIUM SULFATE; ANTIDOTE; CHIMERIC ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; LYSOZYME; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY BCF2; NOXIUSTOXIN; PERIPLASMIC PROTEIN; PROTEIN G; SEPHAROSE; SUCROSE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; DRUG EFFICACY; ESCHERICHIA COLI; FEMALE; LOW TEMPERATURE; MOUSE; NONHUMAN; PRECIPITATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; SCORPION;

BACTERIA (MICROORGANISMS); CENTRUROIDES; CENTRUROIDES NOXIUS; ESCHERICHIA COLI; MURINAE; SCORPIONES;

EID: 1242269847     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2003.10.015     Document Type: Article

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