Overexpression of polyphenol oxidase in transgenic sugarcane results in darker juice and raw sugar

Crop Science

Volumn 45, Issue 1, 2005, Pages 354-362

  Vickers, J E ^a   Grof, C P L ^b   Bonnett, G D ^b   Jackson, P A ^b   Knight, D P ^b   Roberts, S E ^c   Robinson, S P ^b  

^a UNIVERSITY OF SOUTHERN QUEENSLAND   (Australia)

^b CSIRO   (Australia)

^c Davies Lab ^*  (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

SACCHARUM; SACCHARUM HYBRID CULTIVAR;

EID: 12344297640     PISSN: 0011183X     EISSN: None     Source Type: Journal    
DOI: 10.2135/cropsci2005.0354     Document Type: Article

References (27)

1. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl. 1994. Current protocols in molecular biology Vol. 1. John Wiley & Sons Inc., New York.
2. Bachem, C.W.B., G-J. Speckmann, P.C.G. van der Linde, F.T.M. Verheggen, M.D. Hunt, J.C. Steffens, and M. Zabeau.1994. Antisense expression of polyphenol oxidase genes inhibits enzymatic browning in potato tubers. Bio/Technology 12:1101-1105.
3. Beruto, M., P. Curir, and P. Debergh. 1996. Callus growth and somatic embryogenesis in thalamus tissue of Ranunculus asiaticus L. cultivated in vitro: Cytokinin effect and phenol metabolism. In Vitro Cell Dev. Biol. Plant 32:154-160.
4. Bower, R., A.R. Elliot, B.A.M. Potier, and R.G. Birch. 1996. High efficiency, microprojectile-mediated cotransformation of sugarcane, using visible or selectable markers. Mol. Breed. 2:239-249.
5. BSES. 1991. The standard laboratory manual for Australian sugar mills. Vol. 2, Analytical methods and tables. BSES, Brisbane, Australia.
6. Bucheli, C.S., and S.P. Robinson. 1994. Contribution of enzymic browning to color in sugarcane juice. J. Agric. Food Chem. 42:257-261.
7. Bucheli, C.S., I.B. Dry, and S.P. Robinson. 1996. Isolation of a full length cDNA encoding polyphenol oxidase from sugarcane, a C4 grass. Plant Mol. Biol. 31:1233-1238.
8. Christensen, A.H., R.A. Sharrock, and P.H. Quail. 1992. Maize polybiquitin genes: Structure, thermal pertubation of expression and transcript splicing and promotor activity following transfer to protoplasts by electroporation. Plant Mol. Biol. 18:675-689.
9. Coetzer, C., D. Corsini, S. Love, J. Pavek, and N. Tumer. 2001. Control of enzymatic browning in potato (Solanum Tuberosum L.) by sense and antisense RNA from tomato polyphenol oxidase. J. Agric. Food Chem. 49:652-657.
10. Coombs, J., and C.W. Baldry. 1978. Formation of color in cane juice by enzyme catalysed reactions. Part 1. Mechanism and chemical inhibition. Int. Sugar J. 80:291-294.
11. Depicker, A., S. Stachel, P. Dhase, P. Zambryski, and H.M. Goodman. 1982. Nopaline synthase: Transcript mapping and DNA sequence. J. Mol. Appl. Genet. 1:561-573.
12. Dry, I.B., and S.P. Robinson. 1994. Molecular cloning and characterisation of grape berry polyphenol oxidase. Plant Mol. Biol. 26:495-502.
13. Goodacre, B.C., J. Hutson, and J. Coombs. 1980. Enzyme catalysed formation of color in cane juice. Part 3. Inactivation of o-diphenol oxidase by heating intact cane. Distribution of enzyme and color precursors. Int. Sugar J. 82:11-14.
14. Grof, C.P.L., D. Glassop, W.P. Quick, U. Sonnewald, and J.A. Campbell. 1996. Molecular manipulation of sucrose phosphate synthase in sugarcane. p. 124-126. In J.R. Wilson et al. (ed.) Sugarcane: Research towards efficient and sustainable production. CSIRO Division of Tropical Crops and Pastures, Brisbane, Australia.
15. Gross, D., and J. Coombs. 1976. Enzymic color formation in beet and cane juices. Int. Sugar J. 78:106-109.
16. Last, D.I., R.I.S. Brettell, D.A. Chamberlain, A.M. Chaudhury, P.J. Larkin, E.L. Marsh, W.J. Peacock, and E.S. Dennis. 1991. pEmu: An improved vector for gene expression in cereal cells. Theor. Appl. Genet. 81:581-588.
17. Li, L., and J.C. Steffens. 2002. Over-expression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta 215:239-247.
18. Mayer, A.M., and E. Harel. 1979. Polphenol oxidases in plants. Phytochemistry 18:193-215.
19. Napoli, C., C. Lemieux, and R. Jorgensen. 1990. Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279-289.
20. Peters, K.F., C.P.L. Grof, J. Botella, and H. Albert. 1996. Isolation and genetic manipulation of invertase genes in sugarcane. p127-129. In J.R. Wilson et al. (ed.) Sugarcane: Research towards efficient and sustainable production. CSIRO Division of Tropical Crops and Pastures, Brisbane, Australia.
21. Purohit, S.D., A. Sighvi, and K. Tak. 1996. Biochemical characteristics of differentiating callus cultures of Feronia limonia L. Acta Physiol. Plant. 18:47-52.
22. Roberts, S.E., C.P.L. Grof, C.S. Bucheli, S.P. Robinson, and J.R. Wilson. 1996. Genetic engineering of sugarcane for low color raw sugar. p. 130-132. In J.R. Wilson et al. (ed.) Sugarcane: Research towards efficient and sustainable production. CSIRO Division of Tropical Crops and Pastures, Brisbane, Australia.
23. Smith, N.H. 1976. Inhibition of enzymic browning in cane sugar processing. Int. Sugar J. 78:259-263.
24. Tu, C.C. 1977. Effect of cane liming on sugar refinability. Report-Hawaiian Sugar Technologists: 105-108.
25. Vaucheret, H., J.C. Palauqui, T. Elmayan, and B. Moffat. 1995. Molecular and genetic analysis of nitrate reductase co-suppression in transgenic tobacco plants. Mol. Gen. Genet. 248:311-317.
26. Varsha, W.S., C.A. Helliwell, N.A. Smith, W. MingBo, D.T. Rouse, Q. Liu, P.S. Gooding, S.P. Singh, D. Abbott, P.A. Stoutjesdijk, S.P. Robinson, A.P. Gleave A.G. Green, and P.M. Waterhouse. 2001. Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J. 27:581-590.
27. Vaughn, K.C., A.R. Lax, and S.O. Duke. 1988. Polyphenol oxidase: The chloroplast oxidase with no established function. Physiol. Plant. 72:659-665.