Functional analysis of the two Arabidopsis homologues of Toc34, a component of the chloroplast protein import apparatus

Plant Journal

Volumn 23, Issue 6, 2000, Pages 771-783

  Gutensohn, Michael ^a   Schulz, Burkhard ^a,b   Nicolay, Peter ^a   Flügge, Ulf Ingo ^a  

^a UNIVERSITY OF COLOGNE   (Germany)

^b Max Delbruck Laboratorium der Max Planck Gesellschaft   (Germany)

Author keywords

Arabidopsis; Chloroplast; Precursor binding; Protein import; Toc complex; Toc34

Indexed keywords

ANALYTICAL PROCEDURES AND TECHNIQUES; ARABIDOPSIS; CHLOROPLAST; GENE EXPRESSION REGULATION; GUANOSINE TRIPHOSPHATE; PHENOTYPE; PROTEIN INTERACTION; TOC33 GENE; TOC34 GENE;

ANTISENSE ELEMENTS (GENETICS); ARABIDOPSIS; ARABIDOPSIS PROTEINS; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CHLOROPLASTS; CLONING, MOLECULAR; DNA PRIMERS; GENE EXPRESSION; GLUCURONIDASE; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; PROMOTER REGIONS (GENETICS); RNA, MESSENGER;

EID: 0033802314     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2000.00849.x     Document Type: Article

References (46)

1. Basic local alignment search tool
2. The major protein import receptor of plastids is essential for chloroplast biogenesis
3. In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants
4. New plant binary vectors with selectable markers located proximal to the left T-DNA border
5. A protein import receptor in pea chloroplasts, Toc86, is only a proteolytic fragment of a larger polypeptide
6. Identification of a third yeast mitochondrial Tom protein with tetratrico peptide repeats
7. The GTPase superfamily: Conserved structure and molecular mechanism
8. Insertion of the 34-kDa chloroplast protein import component. IAP34, into the chloroplast outer membrane is dependent on its intrinsic GTP-binding capacity
9. Detection of mRNAs in sea urchin embryos by in situ hybridisation using asymmetric RNA probes
10. Developmental regulation of the plastid protein import apparatus
11. Chloroplast protein topogenesis: Import, sorting and assembly
12. In situ hybridisation for the detection of RNA in plant tissues
13. High-throughput RNA extraction from plant samples based on homogenisation by reciprocal shaking in the presence of a mixture of sand and glas beads
14. A new class of plastidic phosphate translocators: A putative link between primary and secondary metabolism by the phosphoenolpyruvate/phosphate antiporter
15. The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: Nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts
16. Mechanism of protein transport across the chloroplast envelope
17. Structure and expression of a plant U1 snRNP 70K gene: Alternative splicing of U1 snRNP 70K pre-mRNAs produces two different transcripts
18. The protein translocation apparatus of chloroplast envelopes
19. The inner membrane of the chloroplast envelope as the site of specific metabolite transport
20. Reconstitution of a chloroplast protein import channel
21. A receptor component of the chloroplast protein translocation machinery
22. Storage of competent cells for Agrobacterium transformation
23. An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana
24. An Arabidopsis mutant defective in the plastid general protein import apparatus
25. Molecular characterization of a carbon transporter in plastids from heterotrophic tissues: The glucose 6-phosphate/phosphate antiporter
26. Protein import into chloroplasts
27. Identification of two GTP-binding proteins in the chloroplast protein import machinery
28. Subcellular location and expression level of a chimeric protein consisting of the maize waxy transit peptide and the β-glucuronidase of Escherichia coli in transgenic potato plants
29. Analysis of the interactions of preproteins with the import machinery over the course of protein import into chloroplasts
30. The preprotein translocation channel of the outer membrane of mitochondria
31. A novel chloroplastic outer membrane-targeting signal that functions at both termini of passenger polypeptides
32. Slow passive diffusion of orthophosphate between intact isolated chloroplasts and suspending medium
33. Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins
34. Tom71, a novel homologue of the mitochondrial preprotein receptor Tom70
35. A consensus nomenclature for the protein-import components of the chloroplast envelope
36. Isolation of components of the chloroplast protein import machinery
37. A constituent of the chloroplast import complex represents a new type of GTP-binding protein
38. Protein translocation into and across the chloroplastic envelope membranes
39. Histochemical localization of β-glucuronidase
40. Toc34 is a preprotein receptor regulated by GTP and phosphorylation
41. A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway
42. Vectors with rare-cutter restriction enzyme sites for expression of open reading frames in transgenic plants
43. Isolation and characterization of a functionally active protein translocation apparatus from chloroplast envelopes
44. Disruption of chloroplast biogenesis and plant development upon down-regulation of a chloroplast processing enzyme involved in the import pathway
45. Identification of chloroplast envelope proteins in close physical proximity to a partially translocated chimeric precursor protein