The use of in situ hybridization to study the transgene pathway following cellular transfection with cationic phosphonolipids

Blood Cells, Molecules, and Diseases

Volumn 30, Issue 1, 2003, Pages 112-123

  Montier, Tristan ^a   Cavalier, Annie ^b   Delépine, Pascal ^a   Guillaume, Christine ^a   Clément, Jean Claude ^c   Yaouanc, Jean Jacques ^c   Morel, Gérard ^d   Thomas, Daniel ^b   Férec, Claude ^a,e  

^a INSERM   (France)

^b UNIVERSITÉ DE RENNES 1   (France)

^c UNIVERSITÉ DE BRETAGNE OCCIDENTALE   (France)

^d UNIVERSITÉ LYON 1   (France)

^e EFSB   (France)

Author keywords

Cationic phosphonolipids; Electron microscopy; Gene therapy; In situ hybridization; Transfection

Indexed keywords

LIPID; PLASMID DNA;

ARTICLE; CELL NUCLEUS; CHEMICAL MODIFICATION; CONTROLLED STUDY; CYTOPLASM; DNA TRANSFER; GENE TRANSFER; GENETIC TRANSFECTION; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; TRANSGENE;

EID: 12244302919     PISSN: 10799796     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1079-9796(03)00017-2     Document Type: Article

References (36)

1. www.pasteur.fr.
2. www4.od.nih.gov/oba/rac/clinicaltrial.htm.
3. Colin M., Maurice M., Trugnan G., Kornprobst M., Harbottle R.P., Knight A., Cooper R.G., Miller A.D., Capeau J., Coutelle C., Brahimi-Horn M.C. Cell delivery, intracellular trafficking and expression of an integrin-mediated gene transfer vector in tracheal epithelial cells. Gene Ther. 7:2000;139-152.
4. Floch V., Audrezet M.P., Guillaume C., Gobin E., Le Bolch G., Clément J.C., Yaouanc J.J., des Abbayes H., Mercier B., Leroy J.P., Abgrall J.F., Férec C. Transgene expression kinetics after transfection with cationic phosphonolipids in hematopoietic non adherent cells. Biochim. Biophys. Acta. 1371:1998;53-70.
5. Floch V., Loisel S., Guenin E., Hervé A.C., Clément J.C., Yaouanc J.J., des Abbayes H., Férec C. Cation substitution in cationic phosphonolipids a new concept to improve transfection activity and decrease cellular toxicity . J. Med. Chem. 43:2000;4617-4628.
6. Audrézet M.P., Le Bolch G., Floch V., Yaouanc J.J., Clément J.C., des Abbayes H., Mercier B., Paul A., Férec C. Novel cationic phosphonolipids agents for in vitro gene transfer to epithelial cell lines. J. Lipos. Res. 7:1997;273-300.
7. Guenin E., Hervé A.C., Floch V., Loisel S., Yaouanc J.J., Clément J.C., Férec C., des Abbayes H. Cationic phosphonolipids containing quaternary phosphonium and arsonium groups for DNA transfection with good efficiency and low cellular toxicity. Angew. Chem. Int. Ed. Engl. 39:2000;629-631.
8. Gable-Guillaume C., Floch V., Mercier B., Audrézet M.P., Gobin E., Le Bolch G., Yaouanc J.J., Clément J.C., des Abbayes H., Leroy J.P., Morin V., Férec C. Cationic phosphonolipids as nonviral gene transfer agents in the lungs of mice. Hum. Gene Ther. 9:1998;2309-2319.
9. Guillaume C., Delépine P., Mercier B., Gobin E., Leroy J.P., Morin V., Férec C. Phosphonocationic lipids in protein delivery to mice lungs. J. Pharm. Sci. 89:2000;639-645.
10. Guillaume C., Delépine P., Droal C., Montier T., Tymen G., Férec C. Characterization and optimization of aerosolized cationic lipid-DNA complexes. Biochem. Biophys. Res. Commun. 286:2001;464-471.
11. Delépine P., Guillaume C., Floch V., Loisel S., Yaouanc J.J., Clément J.C., des Abbayes H., Férec C. Cationic phosphonolipids as nonviral vectors in vitro and in vivo applications . J. Pharm. Sci. 89:2000;629-638.
12. Floch V., Le Bolch G., Gable-Guillaume C., Le Bris N., Yaouanc J.J., des Abbayes H., Férec C., Clément J.C. Phosphonolipids as non-viral vectors for gene therapy. Eur. J. Med. Chem. 33:1998;923-934.
13. Floch V., Legros N., Loisel S., Guillaume C., Guilbot J., Benvegnu T., Ferrieres V., Plusquellec D., Férec C. New biocompatible cationic amphiphiles derivative from glycine betaine a novel family of efficient nonviral gene transfer agents . Biochem. Biophys. Res. Commun. 251:1998;360-365.
14. Lee E.R., Marshall J., Siegel C.S., Jiang C., Yew N.S., Nichols M.R., Nietupski J.B., Ziegler R.J., Lane M.B., Wang K.X., Wan N.C., Scheule R.K., Harris D.J., Smith A.E., Cheng S.H. Detailed analysis of structures and formulations of cationic lipids for efficient gene transfer to the lung. Hum. Gene Ther. 10:1996;1701-1710.
15. Floch V., Delépine P., Guillaume C., Loisel S., Chassé S., Le Bolc'h G., Gobin E., Leroy J.P., Férec C. Systemic administration of cationic lipids/DNA complexes and the relationship between formulation and lung transfection efficiency. Biochim. Biophys. Acta. 1464:2000;95-103.
16. Sitte H., Neumann K., Edelmann L. Cryofixation and cryosubstitution for routine work in transmission electron microscopy. Müller M., Becker R.P., Boyde A., Wolosewick J.J. The Science of Biological Specimen Preparation. 1986;103-118 SEM Inc., AMF O'Hare, Chicago.
17. G. Morel, A. Cavalier, in: L. Williams (Ed.), In Situ Hybridization in Electron Microscopy, CRC Press, Boca Raton, FL, 2001, 455 pp.
18. Felgner J.H., Kummar R., Sridhar C.N., Wheeler C.J., Tsai Y.J., Border R., Ramsay P., Martin M., Felgner P.L. Enhanced gene delivery and mechanism studies with a novel series of cationic lipid formulations. J. Biol. Chem. 269:1994;2550-2561.
19. Farhood H., Serbina N., Huang L. The role of dioleoyl phosphatidylethanolamine in cationic liposome mediated gene transfer. Biochim. Biophys. Acta. 1235:1995;289-295.
20. Bennet M.J., Aberle A.M., Balasubramaniam R.P., Malone J.G., Nantz M.H., Malone R.W. Considerations for the design of improved cationic amphiphile-based transfection reagents. J. Lipos. Res. 6:1996;545-565.
21. Felgner P.L., Gadek T.R., Holm M., Roman R., Chan H.W., Wenz M., Northrop J.P., Ringold G.M., Danielsen M. Lipofection a highly efficient, lipid-mediated DNA-transfection procedure . Proc. Natl. Acad. Sci. USA. 84:1987;7413-7417.
22. Nabel G.J., Nabel E.G., Yang Z.Y., Fox B.A., Plautz G.E., Gao X., Huang L., Shu S., Gordon D., Chang A.E. Direct gene transfer with DNA-liposome complexes in melanoma expression, biologic activity, and lack of toxicity in humans . Proc. Natl. Acad. Sci. USA. 90:1993;11307-11311.
23. Caplen N.J., Alton E.W.F.W., Middleton P.G., Dorin J.R., Stevenson B.J., Gao X., Durham S.R., Jeffery P.K., Hodson M.E., Coutelle C., Huang L., Porteous D.J., Williamson R., Geddes D.M. Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis. Nat. Med. 1:1995;39-46.
24. Ono T., Fujino Y., Tsuchiya T., Tsuda M. Plasmid DNAs directly injected into mouse brain with lipofectin can be incorporated and expressed by brain cells. Neurosci. Lett. 117:1990;259-263.
25. Son K., Huang L. Factors influencing the drug sensitization of human tumor cells for in situ lipofection. Gene Ther. 3:1996;630-634.
26. Raz E., Carson D.A., Parker S.E., Parr T.B., Abai A.M., Aichinger G., Gromkowski S.H., Singh M., Lew D., Yankauchas M.A., Baird A.M., Rhodes G.H. Intradermal gene immunization the possible role of DNA uptake in the induction of cellular immunity to viruses . Proc. Natl. Acad. Sci. USA. 91:1994;9519-9523.
27. Liu Y., Mounkes L.C., Liggit H.D., Brown C.S., Solodin I., Heath T.D., Debs R.J. Factors influencing the efficiency of cationic liposome-mediated intravenous gene delivery. Nat. Biotechnol. 15:1997;167-173.
28. Zhou X., Huang L. DNA transfection mediated by cationic liposomes containing lipopolylysine characterization and mechanism of action . Biochim. Biophys. Acta. 1189:1994;195-203.
29. Ogris M., Steinlein P., Kursa M., Mechtler K., Kircheis R., Wagner E. The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells. Gene Ther. 5:1998;1425-1433.
30. Biggiogera M., Courtens J.L., Derenzini M., Fakan S., Hernandez-Verdun D., Risueno M.C., Soyer-Gobillard M.O. Osmium ammine review of current applications to visualize DNA in electron microscopy . Biol. Cell. 87:1996;121-132.
31. Labat-Moleur F., Steffan A.M., Brisson C., Perron H., Feugeas O., Furstenberg P., Oberling F., Brambilla E., Behr J.P. An electron microscopy study into the mechanism of gene transfer with lipopolyamines. Gene Ther. 3:1996;1010-1017.
32. Zabner J., Fasbender A.J., Moninger T., Poellinger K.A., Welsh M.J. Cellular and molecular barriers to gene transfer by a cationic lipid. J. Biol. Chem. 270:1995;18997-19007.
33. Lechardeur D., Sohn K.J., Haardt M., Joshi P.B., Monck M., Graham R.W., Beatty B., Squire J., O'Brodovich H., Lukacs G.L. Metabolic instability of plasmid DNA in the cytosol a potential barrier to gene transfer . Gene Ther. 6:1999;482-497.
34. Kao G.Y., Change L.J., Allen T.M. Use of targeted cationic liposomes in enhanced DNA delivery to cancer cells. Cancer Gene Ther. 3:1996;250-256.
35. Planck C., Zauner W., Wagner E. Application of membrane-active peptides for drug and gene delivery across cellular membranes. Adv. Drug Deliv. Rev. 34:1998;21-35.
36. Zanta M.A., Belguise-Valladier P., Behr J.P. Gene delivery a single nuclear localization signal peptide is sufficient to carry DNA to the cell nucleus . Proc. Natl. Acad. Sci. USA. 96:1999;91-96.