Nuclear localisation of cytosolic phospholipase A2-α in the EA.hy.926 human endothelial cell line is proliferation dependent and modulated by phosphorylation

Journal of Cell Science

Volumn 115, Issue 23, 2002, Pages 4533-4543

  Grewal, Seema ^a   Morrison, Ewan E ^a   Ponnambalam, Sreenivasan ^a   Walker, John H ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Cytosolic phospholipase A2 ; EA.hy.926; Endothelial; Nucleus

Indexed keywords

ARACHIDONIC ACID; CALCIUM; CELL RECEPTOR; ICOSANOID; LECTIN; LEPTOMYCIN B; LIPID BINDING PROTEIN; MEMBRANE LIPID; MITOGEN ACTIVATED PROTEIN KINASE INHIBITOR; OKADAIC ACID; PHOSPHOLIPASE A2; PHOSPHOPROTEIN PHOSPHATASE INHIBITOR; PROTEIN KINASE INHIBITOR; PROTEIN P42; PROTEIN P44; STAUROSPORINE; SYNAPTOPHYSIN;

AGARICUS BISPORUS; ANGIOGENESIS; CELL GROWTH; CELL MEMBRANE; CELL NUCLEUS; CELL PROLIFERATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOSOL; ENDOTHELIUM CELL; GENE EXPRESSION REGULATION; GENE TRANSLOCATION; HUMAN; HUMAN CELL; NUCLEAR IMPORT; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; REVIEW; SECOND MESSENGER; TUMOR GROWTH;

AMINO ACID SEQUENCE; CELL COUNT; CELL DIVISION; CELL LINE; CELL NUCLEUS; CYTOSOL; FATTY ACIDS, UNSATURATED; FLAVONOIDS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; IMIDAZOLES; LECTINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OKADAIC ACID; PHOSPHOLIPASES A; PHOSPHORYLATION; PROTEIN CONFORMATION; PROTEIN TRANSPORT; PYRIDINES; STAUROSPORINE;

AGARICUS BISPORUS;

EID: 12244284026     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: 10.1242/jcs.00146     Document Type: Review

References (61)

1. Abdullah, K., Cromlish, W. A., Payette, P., Laliberte, F., Huang, Z., Street, I. and Kennedy, B. P. (1995). Human cytosolic phospholipase A2 expressed in insect cells is extensively phosphorylated on Ser-505. Biochim. Biophys. Acta 1244, 157-164.
2. Adam-Klages, S., Schwandner, R., Luschen, S., Ussat, S., Kreder, D. and Kronke, M. (1998). Caspase-mediated inhibition of human cytosolic phospholipase A2 during apoptosis. J. Immunol. 161, 5687-5694.
3. 2+-independent phospholipase A2 augments spontaneous fatty acid release. J. Biol. Chem. 275, 18248-18258.
4. 2+-independent phospholipase A2 but not cytosolic phospholipase A2, which undergoes proteolytic inactivation. J. Biol. Chem. 273, 13870-13877.
5. Barwise, J. L. and Walker, J. H. (1996). Annexins II, IV, V and VI relocate in response to rises in intracellular calcium in human foreskin fibroblasts. J. Cell Sci. 109, 247-255.
6. Borsch-Haubold, A. G., Bartoli, F., Asselin, J., Dudler, T., Kramer, R. M., Apitz-Castro, R., Watson, S. P. and Gelb, M. H. (1998). Identification of the phosphorylation sites of cytosolic phospholipase A2 in agonist-stimulated human platelets and HeLa cells. J. Biol. Chem. 273, 4449-4458.
7. Borsch-Haubold, A. G., Ghomashchi, F., Pasquet, S., Goedert, M., Cohen, P., Gelb, M. H. and Watson, S. P. (1999). Phosphorylation of cytosolic phospholipase A2 in platelets is mediated by multiple stress-activated protein kinase pathways. Eur. J. Biochem. 265, 195-203.
8. Brokstad, K. A., Kalland, K. H., Russell, W. C. and Matthews, D. A. (2001). Mitochondrial protein p32 can accumulate in the nucleus. Biochem. Biophys. Res. Commun. 281, 1161-1169.
9. Buschbeck, M., Ghomashchi, F., Gelb, M. H., Watson, S. P. and Borsch-Haubold, A. G. (1999). Stress stimuli increase calcium-induced arachidonic acid release through phosphorylation of cytosolic phospholipase A2. Biochem. J. 344, 359-366.
10. Carmeliet, P. (2000). Mechanisms of angiogenesis and arteriogenesis. Nat. Med. 6, 389-395.
11. 2+)-dependent translocation domain with homology to PKC and GAP. Cell 65, 1043-1051.
12. Compton, J. L., Bellard, M. and Chambon, P. (1976). Biochemical evidence of variability in the DNA repeat length in the chromatin of higher eukaryotes. Proc. Natl. Acad. Sci. USA 73, 4382-4386.
13. Croxtall, J. D., Choudhury, Q. and Flower, R. J. (1998). Inhibitory effect of peptides derived from the N-terminus of lipocortin 1 on arachidonic acid release and proliferation in the A549 cell line: identification of E-Q-E-Y-V as a crucial component. Br. J. Pharmacol. 123, 975-983.
14. 2 activity in A549 cells. Glucocorticoids block EGF stimulation of cPLA2 phosphorylation. Biochem. Pharmacol. 52, 351-356.
15. 2 expressed in insect cells and present in human monocytes. J. Biol. Chem. 271, 6987-6997.
16. 2-dependent manner. FEBS Lett. 471, 34-38.
17. 2 superfamily of signal transduction enzymes. Trends Biochem. Sci. 22, 1-2.
18. Dessen, A., Tang, J., Schmidt, H., Stahl, M., Clark, J. D., Seehra, J. and Somers, W. S. (1999). Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism. Cell 97, 349-360.
19. 2 translocation to internal membranes. J. Biol. Chem. 276, 30150-30160.
20. 2 activation. J. Leukoc. Biol. 65, 330-336.
21. Gijon, M. A., Spencer, D. M., Kaiser, A. L. and Leslie, C. C. (1999). Role of phosphorylation sites and the C2 domain in regulation of cytosolic phospholipase A2. J. Cell Biol. 145, 1219-1232.
22. 2+. Biochem. J. 353, 503-512.
23. 2 from cytosol to the nuclear envelope in rat basophilic leukemia cells stimulated with calcium ionophore or IgE/antigen. J. Biol. Chem. 270, 15359-15367.
24. Gudmundsdottir, I. J., Halldorsson, H., Magnusdottir, K. and Thorgeirsson, G. (2001). Involvement of MAP kinases in the control of cPLA(2) and arachidonic acid release in endothelial cells. Atherosclerosis 156, 81-90.
25. Hatae, T., Wada, M., Yokoyama, C., Shimonishi, M. and Tanabe, T. (2001). Prostacyclin-dependent apoptosis mediated by PPAR{delta}. J. Biol. Chem. 10, 10.
26. 2 by MNK1-related protein kinases. J. Biol. Chem. 275, 37542-37551.
27. Heggeness, M. H., Wang, K. and Singer, S. J. (1977). Intracellular distributions of mechanochemical proteins in cultured fibroblasts. Proc. Natl. Acad. Sci. USA 74, 3883-3887.
28. 2 by a mitogen-activated protein kinase kinase-independent mechanism in the human astrocytoma cell line 1321N1. Biochem. J. 328, 263-269.
29. 2. J. Biol. Chem. 274, 5163-5169.
30. Hirabayashi, T. and Shimizu, T. (2000). Localization and regulation of cytosolic phospholipase A(2). Biochim. Biophys. Acta 1488, 124-138.
31. Houliston, R. A., Pearson, J. D. and Wheeler-Jones, C. P. (2001). Agonist-specific cross talk between ERKs and p38(mapk) regulates PGI(2) synthesis in endothelium. Am. J. Physiol. Cell. Physiol. 281, C1266-C1276.
32. Jans, D. A. and Hubner, S. (1996). Regulation of protein transport to the nucleus: central role of phosphorylation. Physiol. Rev. 76, 651-685.
33. 2 to the nuclear envelope in acetylcholine-induced prostacyclin synthesis in rabbit coronary endothelial cells. Mol. Pharmacol. 50, 1139-1147.
34. Karin, M. (1992). Signal transduction from cell surface to nucleus in development and disease. FASEB J. 6, 2581-2590.
35. 2 in human platelets. J. Biol. Chem. 268, 26796-26804.
36. 2. J. Biol. Chem. 271, 27723-27729.
37. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
38. 2 is coupled to hormonally regulated release of arachidonic acid. Proc. Natl. Acad. Sci. USA 89, 6147-6151.
39. 2-alpha associates with plasma membrane, endoplasmic reticulum and nuclear membrane in glomerular epithelial cells. Biochem. J. 353, 79-90.
40. Maraldi, N. M., Zini, N., Santi, S. and Manzoli, F. A. (1999). Topology of inositol lipid signal transduction in the nucleus. J. Cell Physiol. 181, 203-217.
41. 2 activation by macrophage migration inhibitory factor (MIF). Regulatory role in cell proliferation and glucocorticoid action. J. Biol. Chem. 274, 18100-18106.
42. 2. J. Biol. Chem. 276, 39653-39660.
43. 2 contribute to mitogenic signaling in myeloblastic leukemia U-937 cells. J. Pharmacol. Exp. Ther 298, 272-278.
44. 2 by p42 microtubule-associated protein 2 kinase and protein kinase C. J. Biol. Chem. 268, 1960-1964.
45. 2 signal. FEBS Lett. 477, 244-248.
46. Pastan, I. and Willingham, M. C. (1985). Endocytosis. Plenum Press.
47. Pedram, A., Razandi, M. and Levin, E. R. (1998). Extracellular signal-regulated protein kinase/Jun kinase cross-talk underlies vascular endothelial cell growth factor-induced endothelial cell proliferation. J. Biol. Chem. 273, 26722-26728.
48. 2. J. Biol. Chem. 274, 14979-14987.
49. 2 to the nuclear envelope elicits topographically localized phospholipid hydrolysis. Biochem. J. 318, 797-803.
50. 2 in regulating arachidonic acid release in macrophages. J. Biol. Chem. 273, 8203-8211.
51. 2 by basic fibroblast growth factor via a p42 mitogen-activated protein kinase-dependent phosphorylation pathway in endothelial cells. J. Biol. Chem. 270, 2360-2366.
52. 2. Eur. J. Biochem. 231, 593-601.
53. 2 to the nuclear envelope and endoplasmic reticulum, J. Biol. Chem. 270, 30749-30754.
54. Sierra-Honigmann, M. R., Bradley, J. R. and Pober, J. S. (1996). "Cytosolic" phospholipase A2 is in the nucleus of sub-confluent endothelial cells but confined to the cytoplasm of confluent endothelial cells and redistributes to the nuclear envelope and cell junctions upon histamine stimulation. Lab. Invest. 74, 684-695.
55. Towbin, H., Staehelin, T. and Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
56. Vane, J. R., Anggard, E. E. and Botting, R. M. (1990). Regulatory functions of the vascular endothelium. N Engl. J. Med. 323, 27-36.
57. Wada, A., Fukuda, M., Mishima, M. and Nishida, E. (1998). Nuclear export of actin: a novel mechanism regulating the subcellular localization of a major cytoskeletal protein. EMBO J. 17, 1635-1641.
58. Whatley, E., Satoh, K., Zimmerman, A., Mcintyre, T. M. and Prescott, S. M. (1994). Proliferation-dependent changes in release of arachidonic acid from endothelial cells. J. Clin. Invest. 94, 1889-1990.
59. 2 in endothelial cells via p42/p44 mitogen-activated protein kinase. FEBS Lett. 420, 28-32.
60. Yamaga, M., Fujii, M., Kamata, H., Hirata, H. and Yagisawa, H. (1999). Phospholipase C-delta1 contains a functional nuclear export signal sequence. J. Biol. Chem. 274, 28537-28541.
61. Yu, L. G., Fernig, D. G., White, M. R., Spiller, D. G., Appleton, P., Evans, R. C., Grierson, I, Smith, J. A., Davies, H., Gerasimenko, O. V. et al. (1999). Edible mushroom (Agaricus bisporus) lectin, which reversibly inhibits epithelial cell proliferation, blocks nuclear localization sequence-dependent nuclear protein import. J. Biol. Chem. 274, 4890-4899.