Immunofluorescent detection and quantitation of hepatitis A virus in sewage treatment effluent and on agri-food surfaces using scanning confocal microscopy

Journal of Virological Methods

Volumn 108, Issue 1, 2003, Pages 9-17

  Kukavica Ibrulj, Irena ^a   Darveau, André ^a   Fliss, Ismaïl ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

Agri food surfaces; Hepatits A virus; Immunofluorescence; Sewage treatment effluent

Indexed keywords

ANTIBODY CONJUGATE; COPPER; HEPATITIS A ANTIBODY; POLYCLONAL ANTIBODY; POLYETHYLENE; POLYVINYLCHLORIDE; STAINLESS STEEL;

ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARTICLE; CHEMICAL LABELING; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CORRELATION ANALYSIS; FOOD; HEPATITIS A VIRUS; IMMUNOFLUORESCENCE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RABBIT; SEWAGE EFFLUENT; SURFACE PROPERTY; VIRUS CONCENTRATION; VIRUS CULTURE; VIRUS DETECTION;

ANIMALS; FLUORESCENT DYES; FOOD MICROBIOLOGY; HEPATITIS A ANTIBODIES; HEPATITIS A VIRUS; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; ORGANIC CHEMICALS; RABBITS; SEWAGE; SURFACE PROPERTIES; VIROLOGY;

HEPATITIS A VIRUS; ORYCTOLAGUS CUNICULUS;

EID: 12244271017     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(02)00227-6     Document Type: Article

References (29)

1. Arnal C., Ferre-Aubineau V., Mignotte B., Imbert-Marcille B.M., Billaudel S. Quantification of hepatitis A virus in shellfish by competitive reverse transcription-PCR with coextraction of standard RNA. Appl. Environ. Microbiol. 65:(1):1999;322-326.
2. Bergh O., Borsheim K.Y., Bratbak G., Heldal M. High abundance of viruses found in aquatic environments. Nature. 340:(6233):1989;467-468.
3. Bettarel Y., Sime-Ngando T., Amblard C., Laveran H. A comparison of methods for counting viruses in aquatic systems. Appl. Environ. Microbiol. 66:(6):2000;2283-2289.
4. Chen F., Lu J.R., Binder B.J., Liu Y.C., Hodson R.E. Application of digital image analysis and flow cytometry to enumerate marine viruses stained with SYBR gold. Appl. Environ. Microbiol. 67:(2):2001;539-545.
5. Cliver D.O. Vehicular transmission of hepatitis A. Public Health Rev. 13:(3-4):1985;235-292.
6. Desenclos J.C., Klontz K.C., Wilder M.H., Nainan O.V., Margolis H.S., Gunn R.A. A multistate outbreak of hepatitis A caused by the consumption of raw oysters. Am. J. Public Health. 81:(10):1991;1268-1272.
7. Drake L.A., Choi K.H., Haskell A.G.E., Dobbs F.C. Vertical profiles of virus-like particles and bacteria in the water column and sediments of Chesapeake Bay, USA. Aquat. Microb. Ecol. 16:1998;17-25.
8. Hadler S.C., Margolis H.S. Viral Hepatitis. 1989;Plenum Medical Book Company, New York, NY.
9. Halliday M.L., Kang L.Y., Zhou T.K., Hu M.D., Pan Q.C., Fu T.Y., Huang Y.S., Hu S.L. An epidemic of hepatitis A attributable to the ingestion of raw clams in Shanghai, China. J. Infect. Dis. 164:(5):1991;852-859.
10. Haugland, R.P. (1998) Special Offer on Alexa Probes. Molecular Probes, Eugene, Oreg.
11. Hennes K.P., Suttle C.A. Direct counts of viruses in natural waters and laboratory cultures by epifluorescence microscopy. Limnol. Oceanogr. 40:1995;1050-1055.
12. Hutin Y.J., Pool V., Cramer E.H., Nainan O.V., Weth J., Williams I.T., Goldstein S.T., Gensheimer K.F., Bell B.P., Shapiro C.N., Alter M.J., Margolis H.S. A multistate, foodborne outbreak of hepatitis A. National Hepatitis A Investigation Team. N. Engl. J. Med. 340:(8):1999;595-602.
13. Jaykus L.A., De Leon R., Sobsey M.D. A virion concentration method for detection of human enteric viruses in oysters by PCR and oligoprobe hybridization. Appl. Environ. Microbiol. 62:(6):1996;2074-2080.
14. Jean J., Blais B., Darveau A., Fliss I. Detection of hepatitis A virus by the nucleic acid sequence-based amplification technique and comparison with reverse transcription-PCR. Appl. Environ. Microbiol. 67:(12):2001;5593-5600.
15. Jean J., Turcotte C., Simard R.E., Fliss I. Production and characterization of polyclonal antibodies against cholecalciferol (vitamin D3). J. Immunol. Methods. 223:(2):1999;155-163.
16. Koff R.S. Hepatitis A. Lancet. 351:(9116):1998;1643-1649.
17. Mbithi J.N., Springthorpe V.S., Boulet J.R., Sattar S.A. Survival of hepatitis A virus on human hands and its transfer on contact with animate and inanimate surfaces. J. Clin. Microbiol. 30:(4):1992;757-763.
18. Mbithi J.N., Springthorpe V.S., Sattar S.A. Effect of relative humidity and air temperature on survival of hepatitis A virus on environmental surfaces. Appl. Environ. Microbiol. 57:(5):1991;1394-1399.
19. Mbithi J.N., Springthorpe V.S., Sattar S.A. Comparative in vivo efficiencies of hand-washing agents against hepatitis A virus (HM-175) and poliovirus type 1 (Sabin). Appl. Environ. Microbiol. 59:(10):1993;3463-3469.
20. Noble R.T., Fuhrman J.A. Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria. Aquat. Microb. Ecol. 14:1998;113-118.
21. Prevot J.S., Dubrou S., Marechal J. Detection of human hepatitis A virus in environmental water by an antigen-capture polymerase chain reaction. Water Sci. Technol. 27:1993;227-233.
22. Proctor L.M., Furhman J.A. Viral mortality of marine bacteria and cyanobacteria. Nature (London). 343:1990;60-62.
23. Sair A.I., D'Souza D.H., Moe C.L., Jaykus L.A. Improved detection of human enteric viruses in foods by RT-PCR. J. Virol. Methods. 100:(1-2):2002;57-69.
24. Sattar S.A., Springthorpe V.S., Karim Y., Loro P. Chemical disinfection of non-porous inanimate surfaces experimentally contaminated with four human pathogenic viruses. Epidemiol. Infect. 102:(3):1989;493-505.
25. Schwab K.J., Neill F.H., Le Guyader F., Estes M.K., Atmar R.L. Development of a reverse transcription-PCR-DNA enzyme immunoassay for detection of 'Norwalk-like' viruses and hepatitis A virus in stool and shellfish. Appl. Environ. Microbiol. 67:(2):2001;742-749.
26. Sobsey M.D., Oglesbee S.E., Wait D.A. Evaluation of methods for concentrating hepatitis A virus from drinking water. Appl. Environ. Microbiol. 50:(6):1985;1457-1463.
27. Sobsey M.D., Shields P.A., Hauchman F.S., Davis A.L., Rullman V.A., Bosch A. Survival and Persistence of Hepatitis A Virus in Environmental Samples. 1988;Alan R. Liss. Inc, New York, NY.
28. Soule H., Genoulaz O., Gratacap-Cavallier B., Chevallier P., Liu Y.C., Seigneurin J.M. Ultrafiltration and reverse transcription-polymerase chain reaction: an efficient process for poliovirus, rotavirus and hepatitis A virus detection in water. Water Res. 34:(3):2000;1063-1067.
29. Wheeler C.M., Fields H.A., Schable C.A., Meinke W.J., Maynard J.E. Adsorption, purification, and growth characteristics of hepatitis A virus strain HAS-15 propagated in fetal rhesus monkey kidney cells. J. Clin. Microbiol. 23:(3):1986;434-440.