A comparison of methods for counting viruses in aquatic systems

Applied and Environmental Microbiology

Volumn 66, Issue 6, 2000, Pages 2283-2289

  Bettarel, Yvan ^a   Sime Ngando, Telesphore ^a   Amblard, Christian ^a   Laveran, Henri ^b  

^a UNIVERSITÉ BLAISE PASCAL   (France)

^b UNIVERSITÉ CLERMONT AUVERGNE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ECOSYSTEM; EFFLUENT; FLUORESCENCE MICROSCOPY; NONHUMAN; NUCLEIC ACID ANALYSIS; SEWAGE; TRANSMISSION ELECTRON MICROSCOPY; VIRUS PARTICLE; WATER CONTAMINATION;

ANIMALS; DEOXYRIBONUCLEASES; ECOSYSTEM; FLUORESCENT DYES; INDOLES; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE; NUCLEIC ACIDS; PLANKTON; RIBONUCLEASES; VIRUSES; WATER MICROBIOLOGY;

T7 PHAGE;

EID: 0034048220     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.66.6.2283-2289.2000     Document Type: Article

References (40)

1. Bergh, O., K. Y. Børsheim, G. Bratbak, and M. Heldal. 1989. High abundance of viruses found in aquatic environments. Nature (London) 340:467-468.
2. Børsheim, K. Y. 1993. Native marine bacteriophages. FEMS Microbiol. Ecol. 102:141-159.
3. Børsheim, K. Y., G. Bratbak, and M. Heldal. 1990. Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy. Appl. Environ. Microbiol. 56:352-356.
4. Bratbak, G., and M. Heldal. 1993. Total count of viruses in aquatic environments, p. 135-138. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.), Handbook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Fla.
5. Drake, L. A., K. H. Choi, A. G. E. Haskell, and F. C. Dobbs. 1998. Vertical profiles of virus-like particles and bacteria in the water column and sediments of Chesapeake Bay, USA. Aquat. Microb. Ecol. 16:17-25.
6. Fenchel, T. 1982. Ecology of heterotrophic microflagellates. IV. Quantitative occurrence and importance as bacterial consumers. Mar. Ecol. Prog. Ser. 9:35-42.
7. Fuhrman, J. A. 1999. Marine viruses and their biogeochemical and ecological effects. Nature (London) 399:541-548.
8. Fuhrman, J. A., and R. T. Noble. 1995. Viruses and protists cause similar bacterial mortality in coastal seawater. Limnol. Oceanogr. 40:1236-1242.
9. Fuhrman, J. A., and C. A. Suttle. 1993. Viruses in marine planktonic systems. Oceanography 6:51-63.
10. Guixa-Boixareu, N., K. Lysnes, and C. Pedros-Alio. 1999. Viral lysis and bacterivory during a phytoplankton bloom in a coastal water microcosm. Appl. Environ. Microbiol. 65:1949-1958.
11. Hara, S., K. Terauchi, and I. Koike. 1991. Abundance of viruses in marine waters: assessment by epifluorescence and transmission electron microscopy. Appl. Environ. Microbiol. 57:2731-2734.
12. Hennes, K. P., and M. Simon. 1995. Significance of bacteriophages for controlling bacterioplankton growth in a mesotrophic lake. Appl. Environ. Microbiol. 61:333-340.
13. Hennes, K. P., and C. A. Suttle. 1995. Direct counts of viruses in natural waters and laboratory cultures by epifluorescence microscopy. Limnol. Oceanogr. 40:1050-1055.
14. Jiang, S. C., and J. H. Paul. 1995. Viral contribution to dissolved DNA in the marine environment as determined by differential centrifugation and kingdom probing. Appl. Environ. Microbiol. 61:317-325.
15. Karl, D. M., and M. D. Bailiff, 1989. The measurement and distribution of dissolved nucleic acids in aquatic environments. Limnol. Oceanogr. 34:543-558.
16. Maranger, R., and D. Bird. 1995. Viral abundance in aquatic systems: a comparison between marine and freshwaters. Mar. Ecol. Prog. Ser. 121:217-226.
17. Marie, D., C. P. D. Brussaard, R. Thyrhaug, G. Bratbak, and D. Vaulot. 1999. Enumeration of marine viruses in culture and natural samples by flow cytometry. Appl. Environ. Microbiol. 65:45-52.
18. Maruyama, A., M. Oda, and T. Higashihara. 1993. Abundance of virus-sized non-DNase-digestible DNA (coated DNA) in eutrophic seawater. Appl. Environ. Microbiol. 59:712-717.
19. McManus, G. B., and J. A. Fuhrman. 1988. Control of marine bacterioplankton populations: measurement and significance of grazing. Hydrobiologia 159:51-62.
20. Noble, R. T., and J. A. Fuhrman. 1998. Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria. Aquat. Microb. Ecol. 14:113-118.
21. Pace, M. L. 1988. Bacterial mortality and the fate of bacterial production. Hydrobiologia 159:41-49.
22. Paul, J. H., S. C. Jiang, and J. B. Rose. 1991. Concentration of viruses and dissolved DNA from aquatic environments by vortex flow filtration. Appl. Environ. Microbiol. 57:2197-2204.
23. Porter, K., and Y. S. Feig. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25:943-948.
24. Proctor, L. M., and J. A. Fuhrman. 1990. Viral mortality of marine bacteria and cyanobacteria. Nature (London) 343:60-62.
25. Servais, P., G. Billen, and J. Vives-Rego. 1985. Rate of bacterial mortality in aquatic environments. Appl. Environ. Microbiol. 49:1448-1454.
26. Sherr, B. F., E. B. Sherr, and C. Pedros-Alio. 1989. Simultaneous measurement of bacterioplankton production and protozoan bacterivory in estuarine waters. Mar. Ecol. Prog. Ser. 54:209-219.
27. Sime-Ngando, T., J.-P. Mignot, C. Amhlard, G. Bourdier, C. Desvilettes, and C. Quiblier-Lloberas. 1996. Characterization of planktonic virus-like particles in a French mountain lake: methodological aspects and preliminary results. Ann. Limnol. 32:1-5.
28. Sime-Ngando, T. 1997. Viruses in aquatic ecosystems. A review. Ann. Biol. 36:181-210.
29. Sokal, R. R., and F. J. Rolf. 1981. Biometry. W. H. Freeman and Co., New York, N.Y.
30. Suttle, C. A., A. M. Chan, and M. T. Cottrell. 1990. Infection of phytoplankton by viruses and reduction of primary productivity. Nature (London) 347: 647-649.
31. Suttle, C. A. 1993. Enumeration and isolation of virus, p. 121-134. In P. F. Kemp, B. F. Sherr, E. B. Sherr, and J. J. Cole (ed.), Handhook of methods in aquatic microbial ecology. Lewis Publishers, Boca Raton, Fla.
32. Suttle, C. A. 1994. The significance of virus to mortality in aquatic microbial communities. Microb. Ecol. 28:237-243.
33. Turk, V., A. S. Rehnstam, E. Lundberg, and A. Hagström. 1992. Release of bacterial DNA by marine nanoflagellates, an intermediate step in phosphorous regeneration. Appl. Environ. Microbiol. 58:3744-3750.
34. Weinbauer, M. G., D. Fuks, S. Puskaric, and P. Peduzzi. 1995. Diel, seasonal and depth-related variability of viruses and dissolved DNA in the northern Adriatic Sea. Microb. Ecol. 30:25-41.
35. Weinbauer, M. G., and C. A. Suttle. 1997. Comparison of epifluorescence and transmission electron microscopy for counting viruses in natural marine waters. Aquat. Microb. Ecol. 13:225-232.
36. Wells, M., and E. D. Goldberg. 1991. Occurrence of small colloids in sea water. Nature (London) 353:342-344.
37. Wilhelm, S. W., M. G. Weinbauer, C. A. Suttle, R. J. Pledger, and D. L. Mitchell. 1998. Measurements of DNA damage and photoreactivation imply that most viruses in marine surface waters are infective. Aquat. Microb. Ecol. 14:215-222.
38. Wilhelm, S.W., and C. A. Suttle. 1999. Viruses and nutrient cycles in the sea. BioScience 49:781-788.
39. Wommack, K. E., R. T. Hill, M. Kessel, E. Russek-Cohen, and R. R. Colwell. 1992. Distribution of viruses in the Chesapeake Bay. Appl. Environ. Microbiol. 31:415-422.
40. Xenopoulos, M., and D. F. Bird. 1997. Virus à la sauce Yo-Pro: microwave enhanced staining for counting viruses by epifluorescence microscopy. Limnol. Oceanogr. 42:1648-1650.