Mapping of carbon flow distribution in the central metabolic pathways of Clostridium cellulolyticum: Direct comparison of bacterial metabolism with a soluble versus an insoluble carbon source

Journal of Microbiology and Biotechnology

Volumn 14, Issue 6, 2004, Pages 1200-1210

  Desvaux, Mickaël ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Bacterial metabolism; Cellobiose; Cellulolytic clostridia; Cellulose; Continuous culture; Metabolic flux analysis

Indexed keywords

ACETIC ACID; ALCOHOL; AMINO ACID; CARBON; CELLOBIOSE; CELLULOSE; CELLULOSOME; EXOPOLYSACCHARIDE; LACTIC ACID; NITROGEN; PHOSPHOGLUCOMUTASE; PYRUVATE SYNTHASE; PYRUVIC ACID;

ARTICLE; BACTERIAL GROWTH; BACTERIAL METABOLISM; CARBON SOURCE; CLOSTRIDIUM CELLULOLYTICUM; CONTROLLED STUDY; FERMENTATION; FLOW MEASUREMENT; NONHUMAN; STOICHIOMETRY;

BACTERIA (MICROORGANISMS); CLOSTRIDIA; CLOSTRIDIUM; CLOSTRIDIUM CELLULOLYTICUM;

EID: 12144283466     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Angelidaki, I., L. Ellegaard and B. K. Ahring. 2003. Applications of the anaerobic digestion process. Adv. Biochem. Eng. Biotechnol. 82: 1-33.
2. Bayer, E. A. and R. Lamed. 1992. The cellulose paradox: Pollutant par excellence and/or a reclaimable natural resource? Biodegradation 3: 171-88.
3. Bélaïch, J. P., C. Tardif, A. Bélaïch, and C. Gaudin. 1997. The cellulolytic system of Clostridium cellulolyticum. J. Biotechnol. 57: 3-14.
4. Boisset, C., H. Chanzy, B. Henrissat, R. Lamed, Y. Shoham, and E. A. Bayer. 1999. Digestion of crystalline cellulose substrates by Clostridium thermocellum cellulosome: Structural and morphological aspects. Biochem. J. 340: 829-835.
5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
6. Chaney, A. L. and E. P. Marbach. 1962. Modified reagents for determination of urea and ammonia. Clin. Chem. 8: 130-132.
7. Desvaux, M. 2001. La fermentation de la cellulose par Clostridium cellulolyticum: Métabolisme modèle d'un Clostridium cellulolytique mésophile. PhD thesis, Université Henri Poincaré-Nancy I, Nancy, France.
8. Desvaux, M., E. Guedon, and H. Petitdemange. 2001. Carbon flux distribution and Kinetics of cellulose fermentation in steady-state continuous cultures of Clostridium cellulolyticum on a chemically defined medium. J. Bacteriol. 183: 119-130.
9. Desvaux, M., E. Guedon and H. Petitdemange. 2000. Cellulose catabolism by Clostridium cellulolyticum growing in batch culture on defined medium. Appl. Environ. Microbiol. 66: 2461-2470.
10. Desvaux, M., E. Guedon, and H. Petitdemange. 2001. Kinetics and metabolism of cellulose degradation at high substrate concentrations in steady-state continuous cultures of Clostridium cellulolyticum on a chemically defined medium. Appl. Environ. Microbiol. 67: 837-845.
11. Desvaux, M., E. Guedon, and H. Petitdemange. 2001. Metabolic flux in cellulose batch and cellulose-fed continuous cultures of Clostridium cellulolyticum in response to acidic environment. Microbiology 147: 1461-1471.
12. Desvaux, M. and H. Petitdemange. 2001. Flux analysis of the metabolism of Clostridium cellulolyticum grown in cellulose-fed continuous culture on a chemically defined medium under ammonium-limited conditions. Appl. Environ. Microbiol. 67: 3846-3851.
13. Dubois, M., K. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1951. A colorimetric method for the determination of sugars. Nature 168: 167.
14. Giallo, J., C. Gaudin, and J. P. Bélaïch. 1985. Metabolism and solubilization of cellulose by Clostridium cellulolyticum H10. Appl. Environ. Microbiol. 49: 1216-1221.
15. Guedon, E., M. Desvaux, and H. Petitdemange. 2002. Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineering. Appl. Environ. Microbiol. 68: 53-58.
16. Guedon, E., M. Desvaux, and H. Petitdemange. 2000. Kinetic analysis of Clostridium cellulolyticum carbohydrate metabolism: Importance of glucose 1-phosphate and glucose 6-phosphate branch points for distribution of carbon fluxes inside and outside cells as revealed by steady-state continuous culture. J. Bacteriol. 182: 2010-2017.
17. Guedon, E., S. Payot, M. Desvaux, and H. Petitdemange. 1999. Carbon and electron flow in Clostridium cellulolyticum grown in chemostat culture on synthetic medium. J. Bacteriol. 181: 3262-3269.
18. Guedon, E., S. Payot, M. Desvaux, and H. Petitdemange. 2000. Relationships between cellobiose catabolism, enzyme levels, and metabolic intermediates in Clostridium cellulolyticum grown in a synthetic medium. Biotechnol. Bioeng. 67: 327-335.
19. Hofman-Bang, J., D. Zheng, P. Westermann, B. K. Ahring, and L. Raskin. 2003. Molecular ecology of anaerobic reactor systems. Adv. Biochem. Eng. Biotechnol. 81: 151-203.
20. Holms, H. 1996. Flux analysis and control of the central metabolic pathways in Escherichia coli. FEMS Microbiol. Reviews 19: 85-116.
21. Huang, L. and C. W. Forsberg. 1990. Cellulose digestion and cellulase regulation and distribution in Fibrobacter succinogenes subsp. succinogenes S85. Appl. Environ. Microbiol. 56: 1221-1228.
22. Kovarova-Kovar, K. and T. Egli. 1998. Growth kinetics of suspended microbial cells: From single-substrate-controlled growth to mixed-substrate kinetics. Microb. Mol. Biol. Rev. 62: 646-666.
23. Leschine, S. B. 1995. Cellulose degradation in anaerobic environments. Annu. Rev. Microbiol. 49: 399-426.
24. Lynd, L. R., J. H. Cushman, R. J. Nichols, and C. E. Wyman. 1991. Fuel ethanol from cellulosic biomass. Science 251: 1318-1323.
25. Lynd, L. R., P. J. Weimer, W. H. van Zyl, and I. S. Pretorius. 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66: 506-577.
26. 1H nuclear magnetic resonance study of glycogen futile cycling in strains of the genus Fibrobacter. Appl. Environ. Microbiol. 64: 74-81.
27. Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal. Chem. 31: 426-428.
28. Mitchell, W. J. 1998. Physiology of carbohydrate to solvent conversion by clostridia. Adv. Microb. Physiol. 39: 31-130.
29. Mokrasch, L. C. 1967. Use of 2,4,6-trinitrobenzenesulfonic acid for the coestimation of amines, amino acids, and proteins in mixtures. Anal. Biochem. 18: 64-71.
30. 2,-fixing cellulolytic bacterium isolated from soil. Int. J. Syst. Microbiol. 51: 123-132.
31. Nandi, R. and S. Sengupta. 1998. Microbial production of hydrogen: An overview. Crit. Rev. Microbiol. 24: 61-84.
32. Payot, S., E. Guedon, C. Cailliez, E. Gelhaye, and H. Petitdemange. 1998. Metabolism of cellobiose by Clostridium cellulolyticum growing in continuous culture: Evidence for decreased NADH reoxidation as a factor limiting growth. Microbiology 144: 375-384.
33. Pereira, A. N., M. Mobedshashi, and M. R. Ladish. 1988. Preparation of cellodextrins. Meth. Enzymol. 160: 26-45.
34. Petitdemange, E., F. Caillet, and C. Gaudin. 1984. Clostridium cellulolyticum sp. nov., a cellulolytic mesophilic species from decayed grass. Int. J. Syst. Microbiol. 34: 155-159.
35. Stephanopoulos, G. 1999. Metabolic fluxes and metabolic engineering. Metab. Eng. 1: 1-11.
36. Strobel, H. J., F. C. Caldwell, and K. A. Dawson. 1995. Carbohydrate transport by the anaerobic thermophile Clostridium thermocellum LQRI. Appl. Environ. Microbiol. 61: 4012-4015.
37. Updegraff, D. M. 1969. Semimicro determination of cellulose in biological materials. Anal. Biochem. 32: 420-424.
38. Weimer, P. J., Y. Shi, and C. L. Odt. 1990. A segmented gas/liquid delivery system for continuous culture of microorganisms on insoluble substrates and its use for growth of Ruminoccus flavefaciens on cellulose. Appl. Microbiol. Biotechnol. 36: 178-183.
39. 2. Geomicrobiol. J. 5: 239-259.
40. Zeng, A. P. 1999. Continuous Culture, 2nd Ed. American Society for Microbiology, Washington D.C., U.S.A.