High-throughput assay for inorganic pyrophosphatases using the cytosolic enzymes of Saccharomyces cerevisiae and human as an example

Protein Expression and Purification

Volumn 18, Issue 3, 2000, Pages 303-309

  Rumsfeld, Jens ^a   Ziegelbauer, Karl ^a   Spaltmann, Frank ^a  

^a BAYER AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANTIFUNGAL ACTIVITY; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CYTOSOL; DIMERIZATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME INHIBITOR; EXPRESSED SEQUENCE TAG; EXPRESSION VECTOR; INORGANIC PYROPHOSPHATASE; MALACHITE GREEN; MOLECULAR CLONING; RECOMBINANT ENZYME;

BOVINAE; ESCHERICHIA COLI; SACCHAROMYCES CEREVISIAE; SIPROETA STELENES;

EID: 0034006116     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1189     Document Type: Article

References (28)

1. Hay R. J. Overview of the treatment of disseminated fungal infections. J. Antimicrob. Chemother. 28:1991;17-25.
2. Georgopapadakou N. H., Walsh T. J. Human mycoses: Drugs and targets for emerging pathogens. Science. 264:1994;371-373.
3. Beck-Sague C. M., Jarvis W. R. Secular trends in the epidemiology of nosocomial fungal infections in the United States, 1980-1990. National Nosocomial Infections Surveillance System. J. Infect. Dis. 167:1993;1247-1251.
4. Ryley J. F., Barrett-Bee K. Sutcliff J. A., Georgopapadakou N. H. Emerging Targets in Antibacterial and Antifungal Chemotherapy. 1992;546-567 Chapman & Hall, New York.
5. Mayor J. S. Dixon G. K., Coppong C. G., Holloman D. W. Antifungal Agents. Discovery and Mode of Action. 1995;9 Bios. Sci. Publ. Ltd. Oxford.
6. Botstein D., Fink G. Yeast: An experimental organism for modern biology. Science. 240:1988;1439-1442.
7. Goffeau. Life with 6000 genes. Science. 274:1996;546.
8. Dujon B. The yeast genome project: What did we learn. Trends Genet. 12:1996;263-270.
9. Spaltmann F., Blunck M., Ziegelbauer K. Computer-aided target selection - Prioritizing targets for antifungal drug discovery. Drug Discov. Today. 4:1999;17-26.
10. Lundin M., Baltscheffski H., Ronne H. Yeast PPA2 gene encodes a mitochondrial inorganic pyrophosphatase that is essential for mitochondrial function. J. Biol. Chem. 266:1991;12168-12172.
11. Chen J., Brevet A., Fromant M., Leveque F., Schmitter J.-M., Blanquet S., Plateau P. Pyrophosphatase is essential for growth of Escherichia coli. J. Bacteriol. 172:1990;5686-5689.
12. Cohen S. A., Sterner R., Keim P. S., Heinrikson R. L. Covalent structural analysis of yeast inorganic pyrophosphatase. J. Biol. Chem. 253:1978;889-897.
13. Stetten D. W. Biosynthesis and pyrophosphate. Am. J. Med. 28:1960;867-870.
14. Kolakowski L. F., Schlösser M., Cooperman B. S. Cloning, molecular characterization and chromosome localization of inorganic pyrophosphatase (PPA) gene from S. cerevisiae. Nucleic Acids Res. 16:1988;10441-10452.
15. Yang Z., Wensel T. G. Molecular cloning and functional expression of cDNA encoding a mammalian inorganic pyrophosphatase. J. Biol. Chem. 267:1992;24641-24647.
16. Yang Z., Wensel T. G. Inorganic pyrophosphatase from bovine retinal rod outer segments. J. Biol. Chem. 267:1992;24634-24640.
17. Lanzetta P. A., Lawrence J. A., Reinach P. S., Candia O. A. An improved assay for nanomole amounts of inorganic phosphate. Anal. Biochem. 100:1979;95-97.
18. Sambrook J., Fritsch E. F., Maniatis T. Molecular Cloning: A Laboratory Manual. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
19. Cha R. S., Thilly W. G. Specificity, efficiency, and fidelity of PCR. PCR Methods Appl. 3:1993;18-29.
20. Springs B., Welsh K. M., Cooperman B. S. Thermodynamics, kinetics and mechanism in yeast inorganic pyrophosphatase catalysis of inorganic pyrophosphate: Inorganic phosphate equilibration. Biochemistry. 20:1981;6384-6391.
21. Welsh K. M., Jakobyansky A. J., Springs B., Cooperman B. S. Catalytic specificity of yeast inorganic pyrophosphatase for magnesium ion as cofactor. An analysis of divalent metal ion and solvent isotope effects on enzyme function. Biochemistry. 22:1983;2243-2248.
22. 2+ inhibition. J. Biol. Chem. 247:1972;7315-7319.
23. i system using a new continuous enzyme assay. Biochim. Biophys. Acta. 429:1976;982-992.
24. Abramochkin G., Shrader T. E. The leucyl/phenylalanyl-tRNA-protein transferase. J. Biol. Chem. 270:1995;20621-20628.
25. Howard K. J., Frank K. B., Sim I. S., Le Grice S. F. J. Reconstitution and properties of homologous and chimeric HIV-1 (HIV-2 p66) p51 reverse transcriptase. J. Biol. Chem. 266:1991;23003-23009.
26. Ueki K., Kincaid R. L. Interchangeable associations of calcineurin regulatory subunit isoforms with mammalian and fungal catalytic subunits. J. Biol. Chem. 268:1993;6554-6559.
27. Winters T. A., Henner W. D., Russell P. S., McCullough A., Jorgensen T. J. Removal of 3′-phosphoglycolate from DNA strand-break damage in an oligonucleotide substrate by recombinant human apurinic/apyrimidinic endonuclease 1. Nucleic Acids Res. 22:1994;1866-1873.
28. Wu D., Ahmed S. N., Lian W., Hersh L. B. Activation of rat choline acetyltransferase by limited proteolysis. J. Biol. Chem. 270:1995;19395-19401.