Identifying cloned Helicobacter pylori promoters by primer extension using a FAM-labelled primer and GeneScan® analysis

Journal of Microbiological Methods

Volumn 60, Issue 3, 2005, Pages 291-298

  Lloyd, Amanda L ^a   Marshall, Barry J ^a   Mee, Brian J ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

FAM; Fluorescent primer; GeneScan ; Primer extension; Promoter identification; Transcriptional start site

Indexed keywords

BACTERIAL RNA; COMPLEMENTARY DNA;

ARTICLE; BACTERIAL GENE; COMPUTER PROGRAM; GENE IDENTIFICATION; GENE SEQUENCE; HELICOBACTER PYLORI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; PURIFICATION; RNA TRANSCRIPTION; SEQUENCE ANALYSIS; TRANSCRIPTION INITIATION SITE;

BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCEINS; GENETIC TECHNIQUES; HELICOBACTER PYLORI; HUMANS; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION, GENETIC;

BACTERIA (MICROORGANISMS); HELICOBACTER PYLORI;

EID: 11844293472     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2004.10.009     Document Type: Article

References (15)

1. E. Altermann, J.R. Klein, and B. Henrich Synthesis and automated detection of fluorescently labelled primer extension products BioTechniques 26 1999 96 98
2. A. Bindereif, and J.B. Neilands Promoter mapping and transcriptional regulation of the iron assimilation system of plasmid ColV-K30 in Escherichia coli K-12 J. Bacteriol. 162 1985 1039 1046
3. J. Brosius Plasmid vectors for the selection of promoters Gene 27 1984 151 160
4. A. Buchet, K. Eichler, and M.A. Mandrand-Berthelot Regulation of the carnitine pathway in Escherichia coli: investigation of the cai-fix divergent promoter region J. Bacteriol. 180 1998 2599 2608
5. I. Delany, R. Rappuoli, and V. Scarlato Fur functions as an activator and as a repressor of putative virulence genes in Neisseria meningitidis Mol. Microbiol. 52 2004 1081 1090
6. R.A. Fekete, M.J. Miller, and D.K. Chattoraj Fluorescently labelled oligonucleotide extension: a rapid and quantitative protocol for primer extension BioTechniques 35 2003 90 94
7. 2+-responsive promoters J. Bacteriol. 181 1999 5516 5520
8. S. Master, T.C. Zahrt, J. Song, and V. Deretic Mapping of Mycobacterium tuberculosis katG promoters and their differential expression in infected macrophages J. Bacteriol. 183 2001 4033 4039
9. A.A. McColm Non primate animal models of H. Pylori infection C.A. Clayton H.L.T. Mobley Helicobacter pylori Protocols 1997 Humana Press Totowa, N.J., USA 235 252
10. R. Rauhut, and G. Klug mRNA degradation in bacteria FEMS Microbiol. Rev. 23 1999 353 370
11. J. Sambrook, and D.W. Russell Molecular Coning: A Laboratory Manual 2001 Cold Spring Harbor Laboratory Press Cold Spring Harbor, New York, USA
12. B.C. Schaefer Revolutions in rapid amplification of cDNA ends: new strategies for polymerase chain reaction cloning of full-length cDNA ends Anal. Biochem. 227 1995 255 273
13. G. Schramm, I. Bruchhaus, and T. Roeder A simple and reliable 5′-RACE approach Nucleic Acids Res. 28 2000 e96
14. K. Takayama, and S. Kjelleberg The role of RNA stability during bacterial stress responses and starvation Environ. Microbiol. 2 2000 355 365
15. M. Yamada, H. Izu, T. Nitta, K. Kurihara, and T. Sakurai High-temperature, nonradioactive primer extension assay for determination of a transcription-initiation site BioTechniques 25 1998 72 74