Regulation of the carnitine pathway in Escherichia coli: Investigation of the cai-fix divergent promoter region

Journal of Bacteriology

Volumn 180, Issue 10, 1998, Pages 2599-2608

  Buchet, Anne ^a   Eichler, Knut ^a,b   Mandrand Berthelot, Marie Andrée ^a  

^a UNIVERSITÉ DE LYON   (France)

^b BASF SE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CARNITINE; CIS ACTING ELEMENT; CYCLIC AMP BINDING PROTEIN; DEOXYRIBONUCLEASE I; GLUCOSE; MESSENGER RNA; NITRATE; REGULATOR PROTEIN;

ANAEROBIC GROWTH; ANAEROBIC METABOLISM; ARTICLE; DNA FOOTPRINTING; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; SIGNAL TRANSDUCTION; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION INITIATION;

ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; TRIXIS;

EID: 0031925159     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.10.2599-2608.1998     Document Type: Article

References (39)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1987. Current protocols in molecular biology. Wiley Interscience, New York, N.Y.
2. Bardonnet, N., and C. Blanco. 1992. 'uid4-antibiotic-resistance cassettes for insertion mutagenesis, gene fusions and genetic constructions. FEMS Microbiol. Lett. 93:243-248.
3. Beck, C. F., and A. J. Warren. 1988. Divergent promoters, a common form of gene organization. Microbiol. Rev. 52:318-326.
4. Begg, Y. A., J. N. Whyte, and B. A. Haddock. 1977. The identification of mutants of Escherichia coli deficient in formate dehydrogenase and nitrate reductase activities using dye indicator plates. FEMS Microbiol. Lett. 2:47-50.
5. Bertoni, G., J. Pérez-Martin, and V. de Lorenzo. 1997. Genetic evidence of separate repressor and activator activities of the Xy1R regulator of the TOL plasmid, pWWO, of Pseudomonas putida. Mol. Microbiol. 23:1221-1227.
6. Bieber, L. L. 1988. Carnitine. Annu. Rev. Biochem. 57:261-283.
7. Brakhage, A. A., M. Wozny, and H. Putzer. 1990. Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase gene. Biochimie 72:725-734.
8. Ebright, R. H. 1993. Transcription activation at class I CAP-dependent promoters. Mol. Microbiol. 8:797-802.
9. Eichler, K., F. Bourgis, A. Buchet, H.-P. Kleber, and M.-A. Mandrand-Berthelot. 1994. Molecular characterisation of the cai operon necessary for carnitine metabolism in Escherichia coli. Mol. Microbiol. 13:775-786.
10. Eichler, K., A. Buchet, F. Bourgis, H.-P. Kleber, and M.-A. Mandrand-Berthelot. 1995. The fix Escherichia coli region contains four genes related to carnitine metabolism. J. Basic Microbiol. 35:217-227.
11. Eichler, K., A. Buchet, R. Lemke, H. P. Kleber, and M. A. Mandrand-Berthelot. 1996. Identification and characterization of the caiF gene encoding a potential transcriptional activator of carnitine metabolism in Escherichia coli. J. Bacteriol. 178:1248-1257.
12. Eichler, K., W.-H. Schunck, H.-P. Kleber, and M.-A. Mandrand-Berthelot. 1994. Cloning, nucleotide sequence, and expression of the Escherichia coli gene encoding carnitine dehydratase. J. Bacteriol. 176:2970-2975.
13. Gennis, R. B., and V. Stewart. 1996. Respiration, p. 217-261. In F. C. Neidhardt, R. Curtiss III, J. L. Ingraham, E. C. C. Lin, K. B. Low, B. Magasanik, W. S. Reznikoff, M. Riley, M. Schaechter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella: cellular and molecular biology. American Society for Microbiology, Washington, D.C.
14. Ghosaini, L., A, Brown, and J. Sturtevant. 1988. Scanning calorimetric study of the thermal unfolding of catabolite activator protein from Escherichia coli in the absence and presence of cyclic mononucleotides. Biochemistry 27: 5257-5261.
15. Gold, S., S. Nishio, S. Tsuyumu, and N. T. Keen. 1992. Analysis of the pelE promoter in Erwinia chrysanthemi EC16. Mol. Plant-Microbe Interact. 5:170-178.
16. Gough, J. A., and N. E. Murray. 1983. Sequence diversity among related genes for recognition of specific targets in DNA molecules. J. Mol. Biol. 166:1-19.
17. Jung, H., K. Jung, and H.-P. Kleber. 1989. Purification and properties of camitine dehydratase from Escherichia coli - a new enzyme of carnitine metabolization. Biochim. Biophys. Acta 1003:270-276.
18. Jung, H., K. Jung, and H.-P. Kleber. 1993. Synthesis of L-carnitine by microorganisms and isolated enzymes. Adv. Biochem. Eng. Biotechnol. 50:21-44.
19. Jung, K., H. Jung, and H.-P. Kleber. 1987. Regulation of L-carnitine metabolism in Escherichia coli. J. Basic Microbiol. 27:131-137.
20. Kleber, H.-P., and H. Aurich. 1985. Stoffwechsel des Carnitins bei Mikroorganismen. Wiss. Z. Karl-Marx-Univ. Leipz. Math.-Naturwiss. Reihe 34:224-237.
21. Kolb, A., S. Busby, H. Buc, S. Garges, and S. Adhya. 1993. Transcriptional regulation by cAMP and its receptor protein. Annu. Rev. Biochem. 62:749-795.
22. Kunkel, T. A., J. D. Roberts, and R. A. Zakour. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154:367-382.
23. 54 (ntrA)-dependent genes is probably united by a common mechanism. Microbiol. Rev. 53:367-376.
24. Lombarde, M. J., A. A. Lee, T. M. Knox, and C. G. Miller. 1997. Regulation of the Salmonella typhimurium pepT gene by cyclic AMP receptor protein (CRP) and FNR acting at a hybrid CRP-FNR site. J. Bacteriol. 179:1909-1917.
25. Maes, M., and E. Messens. 1992. Phenol as grinding material in RNA extractions. Nucleic Acids Res. 20:4374.
26. Miller, J. H. 1992. A short course in bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria, p. 275-278. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
27. Nasser, W., J. Robert-Baudouy, and S. Reverchon. 1997. Antagonistic effect of CRP and KdgR in the transcription control of the Erwinia chrysanthemi pectinolysis genes. Mol. Microbiol. 26:1071-1082.
28. Roth, S., K. Jung, H. Jung, R. K. Hommel, and H.-P. Kleber. 1994. Crotonobetaine reductase from Escherichia coli - a new inducible enzyme of anerobic metabolization of L(-)-carnitine. Antonie van Leeuwenhoek 65: 63-69.
29. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
30. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
31. Seim, H., H. Jung, H. Löster, and H.-P. Kleber. 1985. Wachstumsstimulation und Stoffwechsel des Carnitins bei Escherichia coli.1 Wiss. Z. Karl-Marx-Univ. Leipz. Math.-Naturwiss. Reihe 34:287-292.
32. Seim, H., H. Löster, and H.-P. Kleber. 1982. Reduktiver Stoffwechsel des L-Carnitins und strukturverwandter Trimethylammoniumverbindungen in Escherichia coll. Acta Biol. Med. Ger. 41:1009-1019.
33. Søgaard-Andersen, L., H. Pedersen, B. Holst, and P. Valentin-Hansen. 1991. A novel function of the cAMP-CRP complex in Escherichia coli: cAMP-CRP functions as an adaptor for the CytR repressor in the deo operon. Mol. Microbiol. 5:969-975.
34. Søgaard-Andersen, L., and P. Valentin-Hansen. 1993. Protein-protein interactions in gene regulation: the cAMP-CRP complex sets the specificity of a second DNA-binding protein, the CytR repressor. Cell 75:557-566.
35. Valentin-Hansen, P., B. Albrechtsen, and J. E. Love Larsen. 1986. DNA-protein recognition: demonstration of three genetically separated operator elements that are required for repression of the Escherichia coli deoCABD promoters by the DeoR repressor. EMBO J. 5:2015-2021.
36. Vidal-Ingigliardi, D., and O. Raibaud. 1991. Three adjacent binding sites for cAMP receptor protein are involved in the activation of the divergent malEp-malKp promoters. Proc. Natl. Acad. Sci. USA 88:229-233.
37. Weidenhaupt, M., P. Rossi, C. Beck, H. M. Fischer, and H. Hennecke. 1996. Bradyrhizobium japonicum possesses two discrete sets of electron transfer flavoprotein genes: fixA, fixB and etfS, etfL. Arch Microbiol. 165:169-178.
38. Wu, L. F., and M. A. Mandrand-Berthelot. 1986. Genetic and physiological characterization of new Escherichia coli mutants impaired in hydrogenase activity. Biochimie 68:167-179.
39. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.