Development of a simple and efficient method for assaying cytidine monophosphate sialic acid synthetase activity using an enzymatic reduced nicotinamide adenine dinucleotide/oxidized nicotinamide adenine dinucleotide converting system

Analytical Biochemistry

Volumn 337, Issue 1, 2005, Pages 12-21

  Fujita, Akiko ^a   Sato, Chihiro ^a   Münster Kühnel, Anja K ^b   Gerardy Schahn, Rita ^b   Kitajima, Ken ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

CMP Sia; Cytidine monophosphate sialic acid synthetase; Enzyme activity; High throughput assay system; KDN; Lactate dehydrogenase; Neu5Ac; Sialic acid lyase

Indexed keywords

CARBOXYLIC ACIDS; CATALYSIS; CHEMICAL ACTIVATION; CLONING; CRYSTAL STRUCTURE; NUCLEOTIDES;

CMP-SIA; HIGH-THROUGHPUT ASSAYS; LACTATE DEHYDROGENASE; MONOPHOSPHATES; NEU5AC; SIALIC ACIDS;

ENZYME ACTIVITY;

ACYLNEURAMINATE CYTIDYLYLTRANSFERASE; COMPLEMENTARY DNA; FORMIC ACID; LACTATE DEHYDROGENASE; LACTIC ACID; N ACETYLNEURAMINIC ACID; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; PYROPHOSPHATE; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SIALIC ACID DERIVATIVE;

ACCURACY; ARTICLE; BIOAVAILABILITY; CATALYSIS; CATALYST; COLOR; CONJUGATION; CONTROLLED STUDY; CORRELATION ANALYSIS; CRYSTAL STRUCTURE; ENZYMATIC DEGRADATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME SUBSTRATE; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; MOUSE; MUTATIONAL ANALYSIS; NONHUMAN; OXIDATION KINETICS; PHOTOCHEMISTRY; PRIORITY JOURNAL; RELIABILITY; STRUCTURE ANALYSIS; SYNTHESIS; TECHNIQUE;

ONCORHYNCHUS MYKISS; SALMONIDAE; SIA; VERTEBRATA;

EID: 11844280407     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.10.033     Document Type: Article

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