Molecular cloning of a unique CMP-sialic acid synthetase that effectively utilizes both deaminoneuraminic acid (KDN) and N-acetylneuraminic acid (Neu5Ac) as substrates

Glycobiology

Volumn 11, Issue 8, 2001, Pages 685-692

  Nakata, Daisuke ^a   Münster, Anja K ^b   Gerardy Schahn, Rita ^b   Aoki, Naohito ^a   Matsuda, Tsukasa ^a   Kitajima, Ken ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

CMP KDN synthetase; CMP Neu5Ac synthetase; CMP sialic acid synthatase; Deaminoneuraminic acid; Sialic acid

Indexed keywords

3 KETO 3 DEOXY DEXTRO GLYCERO DEXTRO GALACTONONONIC ACID; AMINO ACID; COMPLEMENTARY DNA; CYTIDINE PHOSPHATE 3 KETO 3 DEOXY DEXTRO GLYCERO DEXTRO GALACTONONONIC ACID; CYTIDINE PHOSPHATE N ACETYLNEURAMINIC ACID; CYTIDINE PHOSPHATE SIALIC ACID SYNTHETASE; CYTIDINE TRIPHOSPHATE; DEAMINONEURAMINIC ACID; MESSENGER RNA; N ACETYLNEURAMINIC ACID; NEURAMINIC ACID DERIVATIVE; RECOMBINANT ENZYME; SIALIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CARCINOGENESIS; CATALYSIS; CONTROLLED STUDY; DEVELOPMENT; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME SUBSTRATE; GENE EXPRESSION; HUMAN; LIVER; MAMMAL; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; NUCLEAR LOCALIZATION SIGNAL; OVARY; PRIORITY JOURNAL; PROTEIN EXPRESSION; REGULATORY MECHANISM; SEQUENCE HOMOLOGY; TESTIS; TISSUE SPECIFICITY;

EID: 0034868247     PISSN: 09596658     EISSN: None     Source Type: Journal    
DOI: 10.1093/glycob/11.8.685     Document Type: Article

References (29)

1. Identification, characterization and developmental expression of a novel α2→8-KDN-transferase which terminates elongation of α2→8-linked oligo-polysialic acid chain synthesis in trout egg polysialoglycoproteins
2. Synthesis of neoglycoconjugates containing deaminated neuraminic acid (KDN) using rat liver α2, 6-sialyltransferase
3. Biosynthesis of KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid). Identification and characterization of a KDN-9-phosphate synthetase activity from trout testis
4. Elevated expression of free deaminoneuraminic acid in mammalian cells cultured in mannose-rich media
5. Rat liver contains age-regulated cytosolic 3-deoxy-D-glycero-galacto-non-2-ulopyranosonic acid (Kdn)
6. Molecular analysis of the biosynthesis pathway of the α2, 8 polysialic acid capsule by Neisseria meningitidis serogroup B
7. Structural determination of a 5-O-methyl-deaminated neuraminic acid (Kdn)-containing polysaccharide isolated from Sinorhizobium fredii
8. Identification of 2-keto-3-deoxy-D-glycero-D-galactonononic acid (KDN, deaminoneuraminic acid) residues in mammalian tissues and human lung carcinoma cells. Chemical evidence of the occurrence of KDN glycoconjugates in mammals
9. Identification of free deaminated sialic acid (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid) in human red blood cells and its elevated expression in fetal cord red blood cells and ovarian cancer cells
10. Monoclonal antibody specific for α2→8-linked oligo deaminated neuraminic acid (KDN) sequences in glycoproteins. Preparation and characterization of a monoclonal antibody and its application in immunohistochemistry
11. Discovery of a new type of sialidase, "KDNase," which specifically hydrolyzes deaminoneuraminyl (3-deoxy-D-glycero-D-galacto-2-nonulosonic acid) but not N-acylneuraminyl linkages
12. Structure of the capsular polysaccharide of Klebsiella ozaenae serotype K4 containing 3-deoxy-D-glycero-D-galacto-nonulosonic acid
13. Cloning and expression of the human N-acetylneuraminic acid phosphate synthase gene with 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid biosynthetic ability
14. Mammalian cytidine 5′-monophosphate N-acetylneuraminic acid synthetase: A nuclear protein with evolutionarily conserved structural motifs
15. A naturally occurring deaminated neuraminic acid, 3-deoxy-D-glycero-D-galacto-nonulosonic acid (KDN). Its unique occurrence at the nonreducing ends of oligosialyl chains in polysialoglycoprotein of rainbow trout eggs
16. PSORT: A program for detecting sorting signals in proteins and predicting their subcellular localization
17. Molecular cloning and expression of the mouse N-acetylneuraminic acid 9-phosphate synthase which does not have deaminoneuraminic acid (KDN) 9-phosphate synthase activity
18. DNA sequencing with chain-terminating inhibitors
19. Identification of Arg-12 in the active site of Escherichia coli K1 CMP-sialic acid synthetase
20. Synthesis of CMP-deaminoneuraminic acid (CMP-KDN) using the CTP:CMP-3-deoxynonulosonate cytidylyltransferase from rainbow trout testis. Identification and characterization of a CMP-KDN synthetase
21. Ultra-rapid detection of mRNAs on northern blots with digoxigenin-labeled oligonucleotides and 'CDP-Star', a new chemiluminescence substrate
22. Purification, cloning and expression of a cytidine 5′-monophosphate N-acetylneuraminic acid synthetase from Haemophilus ducreyi
23. Purification of CMP-N-acetylneuraminic acid synthetase from bovine anterior pituitary glands
24. Isolation and structural elucidation of a novel type of ganglioside, deaminated neuraminic acid (KDN)-containing glycosphingolipid, from rainbow trout sperm. The first example of the natural occurrence of KDN-ganglioside, (KDN)GM3
25. Expression of new KDN-gangliosides in rainbow trout testis during spermatogenesis and their structural identification
26. Occurrence of poly(α2, 8-deaminoneuraminic acid) in mammalian tissues: Widespread and developmentally regulated but highly selective expression on glycoproteins
27. Megalin in normal tissues and carcinoma cells carries oligo/poly α2, 8 deaminoneuraminic acid as a unique posttranslational modification
28. Identification of megalin as the sole rat kidney sialoglycoprotein containing poly α2, 8 deaminoneuraminic acid