Transcriptome variations in human CaCo-2 cells: A model for enterocyte differentiation and its link to iron absorption

Genomics

Volumn 83, Issue 5, 2004, Pages 772-789

  Bédrine Ferran, Hélène ^a   Le Meur, Nolwenn ^b   Gicquel, Isabelle ^a   Le Cunff, Martine ^b   Soriano, Nicolas ^a   Guisle, Isabelle ^b   Mottier, Stéphanie ^a   Monnier, Annabelle ^a   Teusan, Raluca ^b   Fergelot, Patricia ^a   Le Gall, Jean Yves ^a   Léger, Jean ^b   Mosser, Jean ^a  

^a UNIVERSITÉ DE RENNES 1   (France)

^b INSERM   (France)

Author keywords

CaCo 2 cells; Cell differentiation; Enterocytes; Iron overload; Microarray analysis of gene expression; Ontological annotations

Indexed keywords

ACONITASE 1; ADENOSINE TRIPHOSPHATASE (COPPER); CARRIER PROTEIN; FERRITIN; FERROCHELATASE; HEPHAESTIN; PROTEIN; PROTON COUPLED DIVALENT METAL ION TRANSPORTER 2; PROTON COUPLED DIVALENT METAL ION TRANSPORTER 3; TRANSFERRIN; UNCLASSIFIED DRUG;

ARTICLE; CELL ADHESION; CELL CYCLE; CELL DIFFERENTIATION; CELL STRAIN CACO 2; DNA METABOLISM; DNA MICROARRAY; DOWN REGULATION; ERYTHROCYTE; GENE EXPRESSION REGULATION; GENE FUNCTION; HOMEOSTASIS; HUMAN; HUMAN CELL; IMMUNE RESPONSE; IRON ABSORPTION; IRON METABOLISM; IRON TRANSPORT; LIPID METABOLISM; NUCLEOCYTOPLASMIC TRANSPORT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN METABOLISM; SIGNAL TRANSDUCTION; UNINDEXED SEQUENCE; UPREGULATION; XENOBIOTIC METABOLISM;

CACO-2 CELLS; CELL DIFFERENTIATION; ENTEROCYTES; GENE EXPRESSION PROFILING; GENOMICS; HUMANS; IRON; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSCRIPTION, GENETIC;

EID: 11144355607     PISSN: 08887543     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ygeno.2003.11.014     Document Type: Article

References (34)

1. Feder J.N.et al. A novel MHC class I-like gene is mutated in patients with hereditary haemochromatosis. Nat. Genet. 13:1996;399-408.
2. Camaschella C.et al. The gene TFR2 is mutated in a new type of haemochromatosis mapping to 7q22. Nat. Genet. 25:2000;14-15.
3. Njajou O.T.et al. A mutation in SLC11A3 is associated with autosomal dominant hemochromatosis. Nat. Genet. 28:2001;213-214.
4. Kato J.et al. A mutation, in the iron-responsive element of H ferritin mRNA, causing autosomal dominant iron overload. Am. J. Hum. Genet. 69:2001;191-197.
5. Roetto A.et al. Mutant antimicrobial peptide hepcidin is associated with severe juvenile hemochromatosis. Nat. Genet. 33:2003;21-22.
6. Roetto A.et al. Exclusion of ZIRTL as candidate gene of juvenile hemochromatosis and refinement of the critical interval on 1q21. Blood Cells Mol. Dis. 26:2000;205-210.
7. Jouanolle A.M.et al. A candidate gene for hemochromatosis: frequency of the C282Y and H63D mutations. Hum. Genet. 100:1997;544-547.
8. McCune C.A.et al. Hereditary haemochromatosis: only 1% of adult HFEC282Y homozygotes in South Wales have a clinical diagnosis of iron overload. Hum. Genet. 111:2002;538-543.
9. Andrews N.C. Disorders of iron metabolism. N. Engl. J. Med. 341:1999;1986-1995.
10. Waheed A.et al. Association of HFE protein with transferrin receptor in crypt enterocytes of human duodenum. Proc. Natl. Acad. Sci. USA. 96:1999;1579-1584.
11. Griffiths W.J., Kelly A.L., Smith S.J., Cox T.M. Localization of iron transport and regulatory proteins in human cells. Q. J. Med. 93:2000;575-587.
12. Hidalgo I.J., Raub T.J., Borchardt R.T. Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology. 96:1989;736-749.
13. Alvarez-Hernandez X., Nichols G.M., Glass J. Caco-2 cell line: a system for studying intestinal iron transport across epithelial cell monolayers. Biochim. Biophys. Acta. 1070:1991;205-208.
14. Arredondo M., Orellana A., Garate M.A., Nunez M.T. Intracellular iron regulates iron absorption and IRP activity in intestinal epithelial (Caco-2) cells. Am. J. Physiol. 273:1997;G275-G280.
15. Gunshin H.et al. Iron-dependent regulation of the divalent metal ion transporter. FEBS Lett. 509:2001;309-316.
16. Schena M., Shalon D., Davis R.W., Brown P.O. Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science. 270:1995;467-470.
17. Alizadeh A.A.et al. Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature. 403:2000;503-511.
18. Hedenfalk I.et al. Gene-expression profiles in hereditary breast cancer. N. Engl. J. Med. 344:2001;539-548.
19. Diatchenko L.et al. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA. 93:1996;6025-6030.
20. Tusher V.G., Tibshirani R., Chu G. Significance analysis of microarrays applied to the ionizing radiation response. Proc. Natl. Acad. Sci. USA. 98:2001;5116-5121.
21. Perlmutter D.H.et al. The alpha 1-antitrypsin gene is expressed in a human intestinal epithelial cell line. J. Biol. Chem. 264:1989;9485-9490.
22. Muckenthaler M.et al. Relationships and distinctions in iron-regulatory networks responding to interrelated signals. Blood. 101:2003;3690-3698.
23. Hubert N., Hentze M.W. Previously uncharacterized isoforms of divalent metal transporter (DMT)-1: implications for regulation and cellular function. Proc. Natl. Acad. Sci. USA. 99:2002;12345-12350.
24. Jenssen T.K., Laegreid A., Komorowski J., Hovig E. A literature network of human genes for high-throughput analysis of gene expression. Nat. Genet. 28:2001;21-28.
25. Akompong T., Ramm E., Chang C., Yu Z.K., Wessling-Resnick M. Immunological analysis of beta-thalassemic mouse intestinal proteins reveals up-regulation of sucrase-isomaltase in response to iron overload. J. Nutr. 129:1999;949-952.
26. Munoz E.C., Rosado J.L., Lopez P., Furr H.C., Allen L.H. Iron and zinc supplementation improves indicators of vitamin A status of Mexican preschoolers. Am. J. Clin. Nutr. 71:2000;789-794.
27. Le N.T., Richardson D.R. Potent iron chelators increase the mRNA levels of the universal cyclin-dependent kinase inhibitor p21(CIP1/WAF1), but paradoxically inhibit its translation: a potential mechanism of cell cycle dysregulation. Carcinogenesis. 24:2003;1045-1058.
28. McKie A.T.et al. An iron-regulated ferric reductase associated with the absorption of dietary iron. Science. 291:2001;1755-1759.
29. S. Dudoit, Y.H. Yang, M.J. Callow, T.P. Speed, Statistical methods for identifying differentially expressed genes in replicated cDNA microarray experiment, Technical Report 578, Department of Statistics, UC Berkeley, CA, 2000.
30. Mariadason J.M.et al. A gene expression profile that defines colon cell maturation in vitro. Cancer Res. 62:2002;4791-4804.
31. Gehrke S.G.et al. UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood. 101:2003;3288-3293.
32. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215:1990;403-410.
33. Tseng G.C., Oh M.K., Rohlin L., Liao J.C., Wong W.H. Issues in cDNA microarray analysis: quality filtering, channel normalization, models of variations and assessment of gene effects. Nucleic Acids Res. 29:2001;2549-2557.
34. Thénié A.C.et al. Identification of an endogenous RNA transcribed from the antisense strand of the HFE gene. Hum. Mol. Genet. 10:2001;1859-1866.